Stability and Antioxidant Activity of Annatto (Bixa orellana L.) Tocotrienols During Frying and in Fried Tortilla Chips.

Annatto tocotrienols (AnT3), which contain approximately 90% δ-tocotrienol (δ-T3), were added to mid-oleic sunflower oil used for frying tortilla chips over 3 d. The objectives were to evaluate their stability during frying, absorption by the fried food, and activity as antioxidants in frying oil and in tortilla chips during storage. AnT3 did not significantly affect the stability of the oil during frying or the sensory profiles of freshly fried chips. The naturally present α-tocopherol (α-T) in the oil degraded at a lower rate in the presence of AnT3, resulting in significantly higher α-T by the end of the frying study. Levels of tocopherols and tocotrienols in the chips mirrored oil levels. AnT3 did not affect the sensory profile of the chips after 1 wk of storage at 50 °C, but after 3 wk of storage, the control chips had higher levels of painty and rancid flavors compared to chips with AnT3. Headspace hexanal was also significantly higher in the control chips compared to the chips with AnT3 after 3 wk of storage. PRACTICAL APPLICATION Annatto tocotrienols, containing primarily delta- and gamma-tocotrienols, were added to mid-oleic sunflower oil used for frying tortilla chips. The tocotrienols were absorbed by the chips along with the oil. They slowed the degradation of α tocopherol during frying, and reduced levels of painty and rancid flavor scores as well as headspace hexanal in chips that were stored for 3 wk at elevated temperatures. The results indicated that fried snack foods such as tortilla chips may be a suitable and convenient vehicle for enriching tocotrienols in the diet, and that tocotrienols may also enhance the shelf-life of fried foods.

[1]  J. Winkler‐Moser,et al.  Reliability of 1H NMR Analysis for Assessment of Lipid Oxidation at Frying Temperatures , 2017 .

[2]  R. Chess-Williams,et al.  The Role of α1-Adrenoceptor Antagonists in the Treatment of Prostate and Other Cancers. , 2016, International journal of molecular sciences.

[3]  M. Hauer-Jensen,et al.  γ-Tocotrienol as a Promising Countermeasure for Acute Radiation Syndrome: Current Status , 2016, International journal of molecular sciences.

[4]  A. S. Mahoonak,et al.  A Review on Frying: Procedure, Fat, Deterioration Progress and Health Hazards , 2016 .

[5]  S. Vaughn,et al.  Detection of Corn Adulteration in Brazilian Coffee (Coffea arabica) by Tocopherol Profiling and Near-Infrared (NIR) Spectroscopy. , 2015, Journal of agricultural and food chemistry.

[6]  M. Berhow,et al.  Antioxidant Activity of Hybrid Grape Pomace Extracts Derived from Midwestern Grapes in Bulk Oil and Oil-in-Water Emulsions , 2015 .

[7]  E. Bakota,et al.  Antioxidant activities and interactions of α- and γ-tocopherols within canola and soybean oil emulsions , 2014 .

[8]  R. Przybylski,et al.  Frying stability of high oleic sunflower oils as affected by composition of tocopherol isomers and linoleic acid content. , 2013, Food chemistry.

[9]  R. Przybylski,et al.  Frying Performance of Canola Oil Triacylglycerides as Affected by Vegetable Oils Minor Components , 2012 .

[10]  B. Aggarwal,et al.  Tocotrienols, the vitamin E of the 21st century: its potential against cancer and other chronic diseases. , 2010, Biochemical pharmacology.

[11]  Weibiao Zhou,et al.  Performance of palm olein in repeated deep frying and controlled heating processes , 2010 .

[12]  J. Moser,et al.  Frying Stability of Purified Mid-Oleic Sunflower Oil Triacylglycerols with Added Pure Tocopherols and Tocopherol Mixtures , 2009 .

[13]  V. Piironen,et al.  Tocopherol, tocotrienol and plant sterol contents of vegetable oils and industrial fats , 2008 .

[14]  S. Falco,et al.  Frying performance of a sunflower/palm oil blend in comparison with pure palm oil , 2007 .

[15]  P. Robert,et al.  Effect of α-tocopherol, α-tocotrienol and Rosa mosqueta shell extract on the performance of antioxidant-stripped canola oil (Brassica sp.) at high temperature , 2007 .

[16]  R. Moreau,et al.  Reinvestigation of the effect of heat pretreatment of corn fiber and corn germ on the levels of extractable tocopherols and tocotrienols. , 2006, Journal of Agricultural and Food Chemistry.

[17]  L. Skibsted,et al.  Molecular mechanism of antioxidant synergism of tocotrienols and carotenoids in palm oil. , 2006, Journal of agricultural and food chemistry.

[18]  C. Sen,et al.  Tocotrienols: Vitamin E beyond tocopherols. , 2006, Life sciences.

[19]  Lars-Åke Appelqvist,et al.  The chemistry and antioxidant properties of tocopherols and tocotrienols , 1996, Lipids.

[20]  K. Ichihara,et al.  An improved method for rapid analysis of the fatty acids of glycerolipids , 1996, Lipids.

[21]  S. Abidi,et al.  Determination of nonvolatile components in polar fractions of rice bran oils , 2003 .

[22]  I. Elmadfa,et al.  Antioxidative potential of tocotrienols and tocopherols in coconut fat at different oxidation temperatures , 2001 .

[23]  D. Barrera-Arellano,et al.  Loss of tocopherols and formation of degradation compounds in triacylglycerol model systems heated at high temperature , 1999 .

[24]  M. Mozzon,et al.  Identification and estimation of tocotrienols in the annatto lipid fraction by gas chromatography-mass spectrometry , 1998 .

[25]  A. Kamal-Eldin,et al.  Effect of α- and γ-tocopherols on thermal polymerization of purified high-oleic sunflower triacylglycerols , 1998 .

[26]  G. Márquez‐Ruiz,et al.  Regulation of used frying fats and validity of quick tests for discarding the fats , 1998 .

[27]  G. Burton Vitamin E: molecular and biological function , 1994, Proceedings of the Nutrition Society.