Lectin Binding Studies on the Slime Mold, Physarum polycephalum

Cell surface glycoconjugate receptors for wheat germ aggluti-nin, Ricinus communis agglutinin, concanavalin A and soybean agglutinin on two strains of myxoamoebae of Physarum polycephalum with fluorescein isothiocynate-labelled lectins were observed microscopically, but no receptors for Dolichos biflorus agglutinin, Ulex europeus agglutinin-I and Arachis hypogaea agglutinin were found. These results suggest that the myxoamoebae have cell surface glycoproteins with N-glycosidically linked hetero-saccharide chains, but not oligosaccharides with blood group A and H determinants. The number of lectin receptor sites and their affinity constants for lectins were obtained for the myxoamoebae and microplasmodia of P. polycephalum from binding assays with 125I-lectins. The densities of these lectin receptors and the magni-tudes of the affinity constants were similar to those of mammalian cells. Neuraminidase treatment of the myxoamoebae and microplasmodia induced neither a decrease in WGA receptors nor an increase in RCA receptors. These results are strong evidence that sialyl residues are absent in glyco-conjugates on the surface of the myxoamoebae and microplasmodia of P. polycephalum.

[1]  N. Seno,et al.  Difference of Lectin Receptors Between the Free Cell Type and Island Forming Cell Type of Rat Ascites Hepatoma Cells , 1981 .

[2]  Y. Ito,et al.  Derivatization of epoxy-activated agarose with various carbohydrates for the preparation of stable and high-capacity affinity adsorbents: their use for affinity chromatography of carbohydrate-binding proteins. , 1981, Analytical biochemistry.

[3]  M. Monsigny,et al.  Sugar-lectin interactions: how does wheat-germ agglutinin bind sialoglycoconjugates? , 1980, European journal of biochemistry.

[4]  D. L. Clark,et al.  Purification and characterization of multiple species (isolectins) of a slime mold lectin implicated in intercellular adhesion. , 1979, The Journal of biological chemistry.

[5]  A. Tsugita,et al.  A membrane glycoprotein of aggregating Dictyostelium cells with the properties of contact sites. , 1979, European journal of biochemistry.

[6]  I. Goldstein,et al.  The use of fluorescein-conjugated Bandeiraea simplicifolia B4-isolectin as a histochemical reagent for the detection of alpha-D-galactopyranosyl groups. Their occurrence in basement membranes. , 1979, Experimental cell research.

[7]  A. Monroy,et al.  Studies on fertilization in the ascidians. II. Lectin binding to the gametes of Ciona intestinalis. , 1978, Experimental cell research.

[8]  M. Horisberger,et al.  Ultrastructural localization of β-galactan in the nuclei of the myxomycete Physarum polycephalum , 1978 .

[9]  G. Gerisch,et al.  A specific glycoprotein as the target site of adhesion blocking Fab in aggregating Dictyostelium cells , 1978, Nature.

[10]  R. Bourrillon,et al.  Specific modifications of hepatoma cell-surface glycoproteins with enzymes. Effects on in vitro growth as investigated by the use of lectins. , 1978, Experimental cell research.

[11]  T. Butters,et al.  Lectin binding to mosquito Aedes aegyptii and human KB cells: structural comparisons of membrane oligosaccharides. , 1978, Carbohydrate research.

[12]  M. Horisberger,et al.  Structure of a β-galactan isolated from the nuclei of Physarum polycephalum , 1978 .

[13]  M. Glick,et al.  6 – Surface Membranes , 1978 .

[14]  K. Yamazaki,et al.  Extraction of Contractile Protein from Myxoamoebae of Physarum polycephalum , 1978 .

[15]  Hughes Rc,et al.  Effects of neuraminidase on lectin binding by wild-type and ricin-resistant strains of hamster fibroblasts. , 1977 .

[16]  R. Hughes,et al.  Effects of neuraminidase on lectin binding by wild-type and ricin-resistant strains of hamster fibroblasts. , 1977, Biochemistry.

[17]  S. Barondes,et al.  Inhibition of intercellular adhesion in a cellular slime mould by univalent antibody against a cell-surface lectin , 1976, Nature.

[18]  H. Allen,et al.  The isolation of lectins on acid-treated agarose. , 1976, Carbohydrate research.

[19]  V. Stollar,et al.  Sialic acid contents of sindbis virus from vertebrate and mosquito cells. Equivalence of biological and immunological viral properties. , 1976, Virology.

[20]  T. Muramatsu,et al.  Fractionation of glycopeptides by affinity column chromatography on concanavalin A-sepharose. , 1975, Journal of biochemistry.

[21]  S. Barondes,et al.  Cell surface species-specific high affinity receptors for discoidin: developmental regulation in Dictyostelium discoideum. , 1975, Proceedings of the National Academy of Sciences of the United States of America.

[22]  T. Irimura,et al.  Carbohydrate-binding specificity of the so-called galactose-specific phytohemagglutinins. , 1975, Carbohydrate research.

[23]  G. Nicolson,et al.  Qualitative and quantitative interactions of lectins with untreated and neuraminidase-treated normal, wild-type, and temperature-sensitive polyoma-transformed fibroblasts. , 1975, Biochemistry.

[24]  S. Barondes,et al.  Carbohydrate-binding protein from Polysphondylium pallidum implicated in intercellular adhesion. , 1975, Nature.

[25]  S. Barondes,et al.  Carbohydrate-binding protein from Polysphondylium pallidum implicated in intercellular adhesion , 1974, Nature.

[26]  S. Barondes,et al.  Discoidin, a developmentally regulated carbohydrate-binding protein from Dictyostelium discoideum. Purification and characterization. , 1974, Biochemistry.

[27]  S. Kornfeld,et al.  Isolation of the Receptors for Wheat Germ Agglutinin and the Ricinus communis Lectins from Human Erythrocytes Using Affinity Chromatography , 1974 .

[28]  T. Osawa,et al.  Studies on competitive binding of lectins to human erythrocytes. , 1974, Biochemistry.

[29]  T. Osawa,et al.  Specific purification of eel serum and Cytisus sessilifolius anti-H hemagglutinins by affinity chromatography and their binding to human erythrocytes. , 1974, Biochemistry.

[30]  S. Barondes,et al.  Developmentally regulated, carbohydrate-binding protein in Dictyostelium discoideum. , 1973, Proceedings of the National Academy of Sciences of the United States of America.

[31]  L. Sachs,et al.  Difference in the mobility of lectin sites on the surface membrane of normal lymphocytes and malignant lymphoma cells , 1973, International journal of cancer.

[32]  M. Horisberger,et al.  Composition and partial structure of the extracellular polysaccharide of Physarum polycephalum. , 1972, Carbohydrate research.

[33]  H. P. Rusch,et al.  Isolation and Characterization of an Extracellular Polysaccharide from Physarum polycephalum , 1970, Journal of bacteriology.

[34]  T. Steck,et al.  THE BINDING OF KIDNEY-BEAN PHYTOHEMAGGLUTININ BY EHRLICH ASCITES CARCINOMA. , 1965, Biochimica et biophysica acta.

[35]  順子 岡田,et al.  変形菌 Physarum polycephalum の子実体形成 , 1963 .