Expressed sequence tags analysis of Blattella germanica.

Four hundred and sixty five randomly selected clones from a cDNA library of Blattella germanica were partially sequenced and searched using BLAST as a means of analyzing the transcribed sequences of its genome. A total of 363 expressed sequence tags (ESTs) were generated from 465 clones after editing and trimming the vector and ambiguous sequences. About 42% (154/363) of these clones showed significant homology with other data base registered genes. These new B. germanica genes constituted a broad range of transcripts distributed among ribosomal proteins, energy metabolism, allergens, proteases, protease inhibitors, enzymes, translation, cell signaling pathways, and proteins of unknown function. Eighty clones were not well-matched by database searches, and these represent new B. germanica-specific ESTs. Some genes which drew our attention are discussed. The information obtained increases our understanding of the B. germanica genome.

[1]  Ji Young Kim,et al.  Partial characterization of a 29 kDa cysteine protease purified from Taenia solium metacestodes. , 2005, The Korean journal of parasitology.

[2]  R. Aalberse,et al.  Structural biology of allergens. , 2000, The Journal of allergy and clinical immunology.

[3]  C. Marcus,et al.  Cytochrome P450 purification and immunological detection in an insecticide resistant strain of German cockroach (Blattella germanica, L.). , 1998, Insect biochemistry and molecular biology.

[4]  M. Bogliolo,et al.  Drosophila S3 ribosomal protein accelerates repair of 8‐oxoguanine performed by human and mouse cell extracts , 2003, Environmental and molecular mutagenesis.

[5]  C. Horak,et al.  CYP 9 E 2 , CYP 4 C 21 and related pseudogenes from German cockroaches , Blattella germanica : implications for molecular evolution , expression studies and nomenclature of P 450 s , 2001 .

[6]  S. Cha,et al.  The complete nucleotide sequence and gene organization of the mitochondrial genome of the oriental mole cricket, Gryllotalpa orientalis (Orthoptera: Gryllotalpidae). , 2005, Gene.

[7]  V. Sheffield,et al.  cDNA expressed sequence tags of Trypanosoma brucei rhodesiense provide new insights into the biology of the parasite. , 1995, Molecular and biochemical parasitology.

[8]  V. L. Rath,et al.  Structure and Function of the Eukaryotic Ribosome The Next Frontier , 2002, Cell.

[9]  J. Salvaggio,et al.  Protease activity in cockroach and basidiomycete allergen extracts. , 1993, Allergy proceedings : the official journal of regional and state allergy societies.

[10]  M. Wiedmann,et al.  Interaction of the Eukaryotic Elongation Factor 1A with Newly Synthesized Polypeptides* , 2002, The Journal of Biological Chemistry.

[11]  B. Jin,et al.  The mitochondrial genome of the firefly, Pyrocoelia rufa: complete DNA sequence, genome organization, and phylogenetic analysis with other insects. , 2004, Molecular phylogenetics and evolution.

[12]  C. Horak,et al.  CYP9E2, CYP4C21 and related pseudogenes from German cockroaches, Blattella germanica: implications for molecular evolution, expression studies and nomenclature of P450s. , 2001, Gene.

[13]  T. Aigaki,et al.  Longevity determination genes in Drosophila melanogaster , 2002, Mechanisms of Ageing and Development.

[14]  Toru Shimada,et al.  Annotation pattern of ESTs from Spodoptera frugiperda Sf9 cells and analysis of the ribosomal protein genes reveal insect-specific features and unexpectedly low codon usage bias , 2003, Bioinform..

[15]  J. Fox,et al.  Molecular Cloning of a Major Cockroach (Blattella germanica) Allergen, Bla g 2 , 1995, The Journal of Biological Chemistry.

[16]  Nannan Liu,et al.  Overexpression of the cytochrome c oxidase subunit I gene associated with a pyrethroid resistant strain of German cockroaches, Blattella germanica (L.). , 2003, Insect biochemistry and molecular biology.

[17]  Peter F. Hallin,et al.  Genome update: Length distributions of sequenced prokaryotic genomes. , 2004, Microbiology.

[18]  R. Helm,et al.  Isolation and characterization of a clone encoding a major allergen (Bla g Bd90K) involved in IgE-mediated cockroach hypersensitivity. , 1996, The Journal of allergy and clinical immunology.

[19]  I. Wool.  Extraribosomal functions of ribosomal proteins. , 1996, Trends in biochemical sciences.

[20]  D. Petrov,et al.  Genomic gigantism: DNA loss is slow in mountain grasshoppers. , 2001, Molecular biology and evolution.

[21]  D. Cochran,et al.  The German Cockroach, Blattella germanica , 1975 .

[22]  S. Luo,et al.  Sequencing Analysis of cDNA Clones Encoding the American Cockroach Cr-PI Allergens , 1996, The Journal of Biological Chemistry.

[23]  D. Cochran,et al.  Preliminary studies of the chromosomes of twelve cockroach species (Blattaria: Blattidae, Blattellidae, Blaberidae). , 1967, Annals of the Entomological Society of America.

[24]  F. Hegardt,et al.  Cytochrome c oxidase subunit I from the cockroach Blattella germanica: cloning, developmental pattern and tissue expression. , 1994, Insect biochemistry and molecular biology.

[25]  R. Waterston,et al.  Expressed Sequence Tag Analysis of the Bradyzoite Stage of Toxoplasma gondii: Identification of Developmentally Regulated Genes , 1998, Infection and Immunity.

[26]  H. Jäckle,et al.  Cooperative action of antioxidant defense systems in Drosophila , 2001, Current Biology.

[27]  Li Li,et al.  PlasmoDB: the Plasmodium genome resource. A database integrating experimental and computational data , 2003, Nucleic Acids Res..

[28]  P. Turkeltaub,et al.  The important sources of German cockroach allergens as determined by RAST analyses. , 1984, The Journal of allergy and clinical immunology.

[29]  V. Duschak,et al.  Proteinase and gelatinolytic activities of house dust mite and cockroach extracts. , 1999, Journal of investigational allergology & clinical immunology.

[30]  K. H. Byun,et al.  Cloning and expression of trypsin-encoding cDNA from Blattella germanica and its possibility as an allergen. , 2005, The Korean journal of parasitology.