Morphology and adhesion of biomolecules on silicon based surfaces.

Biomolecules such as proteins, on silicon based surfaces are of extreme importance in various applications including microfabricated silicon implants, in the fabrication of microdevices with protein compounds (e.g., biosensors), and therapeutics. Morphology of silicon based surfaces with and without biomolecules and their adhesion with the substrate govern performance and reliability of the biological application. In this research, step by step morphological changes of silicon as well as adhesion during its surface modification have been studied using atomic force microscopy. To improve adhesion between biomolecules and the silicon based surfaces, chemical conjugation as well as surface patterning have been used. Changes in adhesion as a result of surface modification have been analyzed with the help of contact angle measurements. Phase imaging technique was used to confirm the presence of biomolecules on the surface. To understand the relationship between morphology and local values of adhesion, adhesion mapping and local stiffness mapping were carried out.

[1]  Miguel A R B Castanho,et al.  An overview of the biophysical applications of atomic force microscopy. , 2003, Biophysical chemistry.

[2]  H. Hansma,et al.  Applications for atomic force microscopy of DNA. , 1995, Biophysical journal.

[3]  R S Pereira,et al.  Atomic force microscopy as a novel pharmacological tool. , 2001, Biochemical pharmacology.

[4]  M. Radmacher,et al.  Mapping interaction forces with the atomic force microscope. , 1994, Biophysical Journal.

[5]  Bengt Herbert Kasemo,et al.  Biological surface science , 1998 .

[6]  B. Bhushan,et al.  Nanotribological characterization of digital micromirror devices using an atomic force microscope. , 2004, Ultramicroscopy.

[7]  N. Voelcker,et al.  Molecular recognition force spectroscopy , 2005 .

[8]  JohnR. Woodward.  Biosensors: Applications in Medicine, Environmental Protection and Process Control , 1990 .

[9]  H. Butt,et al.  Measuring surface forces in aqueous electrolyte solution with the atomic force microscope , 1995 .

[10]  H. Gaub,et al.  Intermolecular forces and energies between ligands and receptors. , 1994, Science.

[11]  W. Kern.  Cleaning solutions based on hydrogen peroxide for use in silicon semiconductor technology , 1970 .

[12]  R. Smart,et al.  Vitronectin adsorption on surfaces visualized by tapping mode atomic force microscopy. , 2004, Journal of biomedical materials research. Part A.

[13]  Extrand,et al.  An Experimental Study of Contact Angle Hysteresis , 1997, Journal of colloid and interface science.

[14]  Charles M. Lieber,et al.  Covalently functionalized nanotubes as nanometre- sized probes in chemistry and biology , 1998, Nature.

[15]  V. Hlady,et al.  Effects of Discrete Protein-Surface Interactions in Scanning Force Microscopy Adhesion Force Measurements. , 1995, Langmuir : the ACS journal of surfaces and colloids.

[16]  Gil U. Lee,et al.  Direct measurement of the forces between complementary strands of DNA. , 1994, Science.

[17]  Paul K. Hansma,et al.  Tapping mode atomic force microscopy in liquids , 1994 .

[18]  Stefan Seeger,et al.  Surface tension properties of surface-coatings for application in biodiagnostics determined by contact angle measurements , 2003 .

[19]  Hermann E. Gaub,et al.  Adhesive forces between ligand and receptor measured by AFM , 1994 .

[20]  B. Bhushan.  Handbook of micro/nano tribology , 1995 .

[21]  A. Engel,et al.  High resolution imaging of native biological sample surfaces using scanning probe microscopy. , 1997, Current opinion in structural biology.

[22]  Hong Wang,et al.  Quantitative characterization of biomolecular assemblies and interactions using atomic force microscopy. , 2003, Methods.

[23]  A. S. Grove,et al.  General Relationship for the Thermal Oxidation of Silicon , 1965 .

[24]  Frederick H. Silver,et al.  Biomaterials, Medical Devices and Tissue Engineering: An Integrated Approach , 1993 .

[25]  R. Marchant,et al.  Individual plasma proteins detected on rough biomaterials by phase imaging AFM. , 2000, Journal of biomedical materials research.

[26]  Bharat Bhushan,et al.  Use of phase imaging in atomic force microscopy for measurement of viscoelastic contrast in polymer nanocomposites and molecularly thick lubricant films. , 2003, Ultramicroscopy.

[27]  Donald G. Buerk,et al.  Biosensors: Theory and Applications , 1995 .

[28]  G. Seong,et al.  Atomic force microscopy identification of transcription factor NFkappaB bound to streptavidin-pin-holding DNA probe. , 2002, Analytical biochemistry.

[29]  P. Wagner.  Immobilization strategies for biological scanning probe microscopy. , 1998, FEBS letters.