Butanol production ofClostridium acetobutylicumgrown on sugars found in hemicellulose hydrolysates

SummaryClostridium acetobutylicum was grown on a variety of different media and assayed for enhanced butanol production. Butanol values of about 90% of theoretical were obtained when glucose was used as the substrate. Five other sugars were assayed and the order of butanol production using these substrates was cellobiose>mannose>arabinose>xylose >galactose. The addition of calcium carbonate to the media to enhance xylose utilized was beneficial although lower butanol values were obtained.

[1]  J. H. Parish Trends in the biology of fermentation for fuels and chemicals Edited by A Hollaender and others, pp 591. Plenum Press, New York and London. 1980. $65. ISBN 0‐306‐40752‐3 , 1982 .

[2]  R. Maleszka,et al.  Conversion of D-xylose into ethanol by the yeast Pachysolen tannophilus , 1981, Biotechnology Letters.

[3]  T. E. Timell Recent progress in the chemistry of wood hemicelluloses , 1967, Wood Science and Technology.

[4]  T. Miller,et al.  A serum bottle modification of the Hungate technique for cultivating obligate anaerobes. , 1974, Applied microbiology.

[5]  J. Gottschal,et al.  Non-production of acetone and butanol by Clostridiumacetobutylicum during glucose- and ammonium- limitation in continuous culture , 1981, Biotechnology Letters.

[6]  George T. Tsao,et al.  Direct fermentation of D-xylose to ethanol by a xylose-fermentating yeast mutant, Candida sp XF 217 , 1981, Biotechnology Letters.

[7]  R Gay,et al.  Regulation of the NADH and NADPH-ferredoxin oxidoreductases in clostridia of the butyric group. , 1976, Biochimica et biophysica acta.

[8]  J. Saddler,et al.  Enzymatic hydrolysis of cellulose and various pretreated wood fractions , 1982, Biotechnology and bioengineering.

[9]  G. L. Miller Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar , 1959 .

[10]  T. Jeffries Conversion of xylose to ethanol under aerobic conditions by Candida tropicalis , 1981, Biotechnology Letters.

[11]  R. G. Ackman,et al.  Porous polymer bead packings and formic acid vapor in the GLC of volatile free fatty acids. , 1972, Journal of chromatographic science.

[12]  J. Saddler,et al.  Production of 2,3-butanediol by Klebsiella pneumoniae grown on acid hydrolyzed wood hemicellulose , 1982, Biotechnology Letters.

[13]  R. Davies,et al.  Studies on the acetone-butyl alcohol fermentation: Nutritional and other factors involved in the preparation of active suspensions of Cl. acetobutylicum (Weizmann). , 1941, The Biochemical journal.