Construction and Expression of Two Cytoplasmic hnRNPK Mutants

Heterogeneous nuclear ribonucleoprotein K (hnRNPK) is a member of the hnRNP family which is a DNA/RNA binding protein shuttling between nucleus and cytoplasm but mainly located in the nuleus in most physiological conditions. Previous studies from our lab and the others have shown that hnRNPK is upregulated and displays abnormal cytoplasmic localization in the lung adrenocarcinoma tissue. The functional significance and its related mechanisms of cytoplasmic hnRNPK in the physiopathology of cancer remains still elusive. In order to address this question, we have constructed two hnRNPK mutated forms lacking respectively the nuclear localization signal (NLS) and amino acids 323-337, and examined their subcellular distribution in lung adenocarcinoma H1299 cells in comparison with wild type hnRNPK. This provides appropriate tools for further studies aiming to assess the functions of the cytoplasmic hnRNPK in the physiopathology of cancer and elucidate the related mechanisms.

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