Identification by Genotyping of a Commercial Antigen Preparation as the Source of a Laboratory Contamination with Coxiella burnetii and as an Unexpected Rich Source of Control DNA

ABSTRACT By performing genotyping, a laboratory contamination involving Q fever was traced back to the antigen preparation used in a commercially available complement fixation test. It was established that such antigen preparations contain relatively high loads of DNA/RNA, making them potential sources of contamination but also convenient preparations for control material.

[1]  W. Melchers,et al.  Interlaboratory Evaluation of Different Extraction and Real-Time PCR Methods for Detection of Coxiella burnetii DNA in Serum , 2010, Journal of Clinical Microbiology.

[2]  E. V. van Hannen,et al.  Real-Time PCR with Serum Samples Is Indispensable for Early Diagnosis of Acute Q Fever , 2009, Clinical and Vaccine Immunology.

[3]  M. Nabuurs-Franssen,et al.  Multigenotype Q Fever Outbreak, the Netherlands , 2009, Emerging infectious diseases.

[4]  P. Devine.  Diagnosis of Q Fever , 1998, Journal of Clinical Microbiology.

[5]  F Nistal de Paz,et al.  [Q fever]. , 1994, Medicina clinica.