ABCA3‐mediated choline‐phospholipids uptake into intracellular vesicles in A549 cells

ABCA3 is proposed to function as a lung surfactant lipid transporter. Here we report ABCA3‐dependent lipid uptake into intracellular vesicles in lung adenocarcinoma A549 cells. A549 cells stably expressing GFP‐tagged wild‐type ABCA3 (A549/ABCA3WT) had larger LAMP3‐positive vesicles than their parental cells as well as A549 cells expressing a Walker A motif mutant (A549/ABCA3N568D). The choline‐phospholipids level in A549/ABCA3WT was increased 1.25‐fold compared to that in A549 and A549/ABCA3N568D cells, while the cholesterol levels were similar. Sucrose gradient fractionation analysis in A549/ABCA3WT cells revealed that choline‐phospholipids were enriched in low‐density and nile red‐positive vesicles. Electronmicroscopic analysis showed multilamellar vesicles in A549/ABCA3WT cells. These results indicate that ABCA3 mediates ATP‐dependent choline‐phospholipids uptake into intracellular vesicles.

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