SPECIAL ARTICLE Standards and Guidelines for Validating Next-Generation Sequencing Bioinformatics Pipelines A Joint Recommendation of the Association for Molecular Pathology and the College of American Pathologists

From the Next Generation Sequencing Bioinformatics Pipeline Validation Working Group of the Clinical Practice Committee,* The Association for Molecular Pathology,zz Bethesda, Maryland; the Division of Molecular and Genomic Pathology,y University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania; PathGroup,z Nashville, Tennessee; Genetics and Genomics Center,x Icahn School of Medicine, Mount Sinai Hospital, New York, New York; Sema4 Laboratory,{ Branford, Connecticut; the Division of Biomedical Statistics and Informatics,k Department of Health Sciences Research, Mayo Clinic, Rochester, Minnesota; Invitae,** San Francisco, California; Color Genomics, Inc.,yy Burlingame, California; ARUP Laboratories,xx Salt Lake City, Utah; and the Department of Pathology and Laboratory Medicine,{{ Children’s Healthcare of Atlanta, Atlanta, Georgia

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