Bioremediation of Agro-Based Pulp Mill Effluent by Microbial Consortium Comprising Autochthonous Bacteria

Small-scale agro-based pulp and paper mills are characterized as highly polluting industries. These mills use Kraft pulping process for paper manufacturing due to which toxic lignified chemicals are released into the environment. Lack of infrastructure, technical manpower, and research and development facilities restricts these mills to recover these chemicals. Therefore, the chemical oxygen demand (COD) of the emanating stream is quite high. For solving the above problem, four bacteria were isolated from the premises of agro-based pulp and paper mill which were identified as species of Pseudomonas, Bacillus, Pannonibacter, and Ochrobacterum. These bacteria were found capable of reducing COD up to 85%–86.5% in case of back water and 65-66% in case of back water : black liquor (60 : 40), respectively, after acclimatization under optimized conditions (pH 6.8, temperature 35°C, and shaking 200 rpm) when the wastewater was supplemented with nitrogen and phosphorus as trace elements.

[1]  K. Schleifer,et al.  Phylogenetic identification and in situ detection of individual microbial cells without cultivation. , 1995, Microbiological reviews.

[2]  Awwa,et al.  Standard Methods for the examination of water and wastewater , 1999 .

[3]  Katja Schumacher,et al.  India's pulp and paper industry: Productivity and energy efficiency , 1999 .

[4]  T. Sreekrishnan,et al.  Aquatic toxicity from pulp and paper mill effluents: a review , 2001 .

[5]  T. Viraraghavan,et al.  Treatment of pulp and paper mill wastewater--a review. , 2004, The Science of the total environment.

[6]  R. Kirby.  Actinomycetes and Lignin Degradation. , 2005, Advances in applied microbiology.

[7]  S. Mansfield,et al.  Colour remediation of pulp mill effluent using purified fungal cellobiose dehydrogenase: reaction optimisation and mechanism of degradation. , 2005, Biotechnology and bioengineering.

[8]  I. D. Mall,et al.  Electrochemical Degradation of Pulp and Paper Mill Wastewater. Part 1. COD and Color Removal , 2006 .

[9]  R. Zytner,et al.  COMBINING ULTRAFILTRATION PROCESS WITH COAGULATION PRETREATMENT FOR PULP MILL WASTEWATER TREATMENT , 2007, Environmental technology.

[10]  R. Ocampo-Pérez,et al.  Adsorption of Fluoride from Water Solution on Bone Char , 2007 .

[11]  H. Purohit,et al.  Biodegradation of kraft-lignin by Bacillus sp. isolated from sludge of pulp and paper mill , 2007, Biodegradation.

[12]  E. Hafez,et al.  Molecular Characterization of Two Native Egyptian Ligninolytic Bacterial Strains , 2008 .

[13]  F. Kargı,et al.  Advanced oxidation treatment of pulp mill effluent for TOC and toxicity removals. , 2008, Journal of environmental management.

[14]  R. Chandra,et al.  Investigation of the biotransformation of pentachlorophenol and pulp paper mill effluent decolorisation by the bacterial strains in a mixed culture. , 2008, Bioresource technology.

[15]  Ram Chandra,et al.  Reduction of pollutants in pulp paper mill effluent treated by PCP-degrading bacterial strains , 2009, Environmental monitoring and assessment.

[16]  T. Rocha-Santos,et al.  Biological treatment of the effluent from a bleached kraft pulp mill using basidiomycete and zygomycete fungi. , 2009, The Science of the total environment.

[17]  Wenting Zhang,et al.  Isolation, identification and application in lignin degradation of an ascomycete GHJ-4 , 2011 .

[18]  Valeria Panebianco,et al.  Supplementary Figure 2 , 2012 .