Three-dimensional image generation method of single cells based on optical flow analysis

The present invention discloses a method of generating three-dimensional image of single cells based on the optical flow analysis. The method of the present invention has the following advantages: 1) achieved by microscopic vision algorithms to enhance light-induced dielectrophoresis feedback control function can be manipulated platform; 2) for tracking the movement of the three-dimensional cell motion detection method based on optical flow field, thereby complete cell kinetics model parameter estimation; 3) by controlling the rotation of single cells, the cells acquire a two-dimensional image sequence, achieve maximum likelihood estimation method based on three-dimensional image generation techniques sequence of two-dimensional images of cells controlled single cells. The method of the present invention, its equipment simple, low cost, no phototoxic effect, the requirements of the sample is low, the operability is improved.