Carbohydrate Composition of β-Core

Abstract β-Core is a major component of the hCG-related molecules found in pregnancy urine. We previously have purified the β-core molecule and have deduced portions of its carbohydrate structure based on lectin binding data. In the present study we used recently developed technology to determine the carbohydrate composition of β-core and hCGβ (CR119). For direct compositional analysis, parallel samples were hydrolyzed in trifluoroacetic acid and analyzed for sialic acid and neutral sugars without prior derivitization. Separation of the monosaccharides was achieved by HPLC on a Dionex CarboPac column eluted at high pH, and the resolved monosaccharides were quantified by pulsed amperometric detection. The amounts of sugar that were found relative to peptide indicated the presence of two N-linked oligosaccharides per molecule on both β-core and hCGβ. hCGβ contained additional sugars consistent with the presence of four O-linked oligosaccharides. Compared to hCGβ, β-core contained negligible sialic acid, gal...

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