Ochratoxin A mimotope from second-generation peptide library and its application in immunoassay.

With the advantage of replacing mycotoxins and their conjugates, mimotopes have been applied to immunoassays, the most common of which were seleted from random phage displayed peptide libraries. However, these mimotopes were limited by the diversities of the peptide libraries. The aim of this study was to demonstrate that a variety of mimotopes can be obtained by constructing a second-generation peptide library. Using mycotoxin ochratoxin A as a model system, a dodecapeptide mimotope was isolated after panning the second-generation peptide library. The half inhibition concentration of the chemiluminescent enzyme-linked immunosorbent assay setup with this mimotope was 0.04 ng/mL, and the linear range was 0.006-0.245 ng/mL. The mimotope was also used to develop a qualitative dipstick assay with a cutoff level of 1 ng/mL. The method not only presents a high sensitivity but also contributes to the development of mimotope-based assays for mycotoxins avoiding the need of synthesizing toxic mycotoxin conjugates.

[1]  Y. Sugita‐Konishi,et al.  Site-specific in vivo mutagenicity in the kidney of gpt delta rats given a carcinogenic dose of ochratoxin A. , 2011, Toxicological sciences : an official journal of the Society of Toxicology.

[2]  Zhongming Zheng,et al.  Validation of an ELISA test kit for the detection of ochratoxin A in several food commodities by comparison with HPLC , 2005, Mycopathologia.

[3]  Steven J Brown,et al.  Isolation of Erythropoietin Receptor Agonist Peptides Using Evolved Phage Libraries , 1998, Biological chemistry.

[4]  Xu Yang,et al.  Development of a colloidal gold strip for rapid detection of ochratoxin A with mimotope peptide , 2009 .

[5]  Yang Xu,et al.  Simultaneous multiresidue determination of mycotoxins in cereal samples by polyvinylidene fluoride membrane based dot immunoassay , 2012 .

[6]  Jin-Ho Seo,et al.  Production of a monoclonal antibody against ochratoxin A and its application to immunochromatographic assay. , 2005, Journal of agricultural and food chemistry.

[7]  K. A. Noren,et al.  Construction of high-complexity combinatorial phage display peptide libraries. , 2001, Methods.

[8]  A. Plückthun,et al.  Computational analysis of off-rate selection experiments to optimize affinity maturation by directed evolution. , 2010, Protein engineering, design & selection : PEDS.

[9]  Christopher J Roberts,et al.  Comparative effects of pH and ionic strength on protein-protein interactions, unfolding, and aggregation for IgG1 antibodies. , 2010, Journal of pharmaceutical sciences.

[10]  Keisuke Fukunaga,et al.  Practical Tips for Construction of Custom Peptide Libraries and Affinity Selection by Using Commercially Available Phage Display Cloning Systems , 2012, Journal of nucleic acids.

[11]  G. Reddy,et al.  Phage‐displayed peptides that mimic aflatoxin B1 in serological reactivity , 2001, Journal of applied microbiology.

[12]  Y. Ueno,et al.  A comparison between enzyme immunoassay and HPLC for ochratoxin A detection in green, roasted and instant coffee , 2007 .

[13]  G. Nolan,et al.  Evolution of peptides that modulate the spectral qualities of bound, small-molecule fluorophores. , 1998, Chemistry & biology.

[14]  M. Nakajima,et al.  Determination of ochratoxin A in green coffee by immunoaffinity column cleanup and liquid chomatography: collaborative study. , 2005, Journal of AOAC International.

[15]  R. May,et al.  High Affinity Mimotope of the Polysaccharide Capsule of Cryptococcus neoformans Identified from an Evolutionary Phage Peptide Library1 , 2002, The Journal of Immunology.

[16]  Yanping Li,et al.  Phage-displayed peptides that mimic zearalenone and its application in immunoassay , 2011 .

[17]  Alexandr E. Urusov,et al.  Immunochromatographic assay for the detection of ochratoxin A , 2011 .

[18]  John E. Linz,et al.  Identification of Mimotope Peptides Which Bind to the Mycotoxin Deoxynivalenol-Specific Monoclonal Antibody , 1999, Applied and Environmental Microbiology.

[19]  V. Roschke,et al.  Discovery of high‐affinity peptide binders to BLyS by phage display , 2005, Journal of molecular recognition : JMR.

[20]  Elena Lizarraga,et al.  Quantification of ochratoxin A and five analogs in Navarra red wines , 2012 .

[21]  Yan Zhang,et al.  Enzyme-linked immunosorbent assay and colloidal gold immunoassay for ochratoxin A: investigation of analytical conditions and sample matrix on assay performance , 2007, Analytical and bioanalytical chemistry.

[22]  F. Yu,et al.  Development of a monoclonal antibody against ochratoxin A and its application in enzyme-linked immunosorbent assay and gold nanoparticle immunochromatographic strip. , 2008, Analytical chemistry.

[23]  F. Yu,et al.  Comparison of enzyme-linked immunosorbent assays with chemiluminescent and colorimetric detection for the determination of ochratoxin A in food. , 2011, Journal of agricultural and food chemistry.

[24]  C. Lino,et al.  Determination of ochratoxin A in Portuguese rice samples by high performance liquid chromatography with fluorescence detection , 2005, Analytical and bioanalytical chemistry.

[25]  F. Palmisano,et al.  Determination of ochratoxin A in green coffee beans by solid-phase microextraction and liquid chromatography with fluorescence detection. , 2008, Journal of chromatography. A.

[26]  W. Dower,et al.  Membrane insertion defects caused by positive charges in the early mature region of protein pIII of filamentous phage fd can be corrected by prlA suppressors , 1994, Journal of bacteriology.

[27]  S. Dübel,et al.  Affinity maturation by phage display. , 2009, Methods in molecular biology.

[28]  N. Hughes-Jones,et al.  THE EFFECT OF PH AND IONIC STRENGTH ON THE REACTION BETWEEN ANTI-D AND ERYTHROCYTES. , 1964, Immunology.

[29]  C. Maragos,et al.  Production and characterization of anti-idiotype and anti-anti-idiotype antibodies against fumonisin B1 , 1995 .

[30]  F. Longobardi,et al.  Fluorescence polarization immunoassay for rapid screening of ochratoxin A in red wine , 2009, Analytical and bioanalytical chemistry.

[31]  R K Wilson.  High-throughput purification of M13 templates for DNA sequencing. , 1993, BioTechniques.

[32]  Zhou Yu,et al.  An immunoassay for ochratoxin A without the mycotoxin , 2007 .

[33]  B. Hammock,et al.  Phage-displayed peptide that mimics aflatoxins and its application in immunoassay. , 2013, Journal of agricultural and food chemistry.

[34]  G. Rappocciolo,et al.  Monoclonal anti-idiotype induces protection against the cytotoxicity of the trichothecene mycotoxin T-2. , 1990, Journal of immunology.

[35]  M. Shamtsyan.  Food legislation and its harmonization in Russia. , 2014, The Journal of the Science of Food and Agriculture.

[36]  Veronica M T Lattanzio,et al.  Simultaneous LC–MS/MS determination of aflatoxin M1, ochratoxin A, deoxynivalenol, de-epoxydeoxynivalenol, α and β-zearalenols and fumonisin B1 in urine as a multi-biomarker method to assess exposure to mycotoxins , 2011, Analytical and bioanalytical chemistry.

[37]  T. Lövgren,et al.  Synthetic single-framework antibody library integrated with rapid affinity maturation by VL shuffling. , 2011, Protein engineering, design & selection : PEDS.