Microalgal polyunsaturated fatty acids: Hotspots and production techniques

Algae play a crucial role in the earth’s primary productivity by producing not only oxygen but also a variety of high-value nutrients. One such nutrient is polyunsaturated fatty acids (PUFAs), which are accumulated in many algae and can be consumed by animals through the food chain and eventually by humans. Omega-3 and omega-6 PUFAs are essential nutrients for human and animal health. However, compared with plants and aquatic sourced PUFA, the production of PUFA-rich oil from microalgae is still in the early stages of exploration. This study has collected recent reports on algae-based PUFA production and analyzed related research hotspots and directions, including algae cultivation, lipids extraction, lipids purification, and PUFA enrichment processes. The entire technological process for the extraction, purification and enrichment of PUFA oils from algae is systemically summarized in this review, providing important guidance and technical reference for scientific research and industrialization of algae-based PUFA production.

[1]  Xinyu Liu,et al.  Highly efficient co-production of fucoxanthin and eicosapentaenoic acid by heterotrophic cultivation of a newly isolated microalga Nitzschia sp. FZU62 , 2023, Algal Research.

[2]  Hongli Zheng,et al.  Enhancing Docosahexaenoic Acid Production of Isochrysis galbana from Starch-Rich Food Processing Byproducts , 2023, Fermentation.

[3]  P. Capek,et al.  Structural properties of the extracellular biopolymer (β-D-xylo-α-D-mannan) produced by the green microalga Gloeocystis vesiculosa Nägeli. , 2023, Carbohydrate research.

[4]  C. Roman,et al.  Advances in Biodiesel Production from Microalgae , 2023, Energies.

[5]  F. Khodaiyan,et al.  An investigation on conjugated linoleic acid content, fatty acid composition, and physicochemical characteristics of Iranian Kurdish butter oil , 2022, Food science & nutrition.

[6]  M. Liu,et al.  From lab to application: Cultivating limnetic microalgae in seawater coupled with wastewater for biodiesel production on a pilot scale. , 2022, Water research.

[7]  Tianpei Li,et al.  Biological hydrogen with industrial potential: Improvement and prospection in biohydrogen production , 2022, Journal of Cleaner Production.

[8]  Jo‐Shu Chang,et al.  Enhanced production of high-value polyunsaturated fatty acids (PUFAs) from potential thraustochytrid Aurantiochytrium sp. , 2022, Bioresource technology.

[9]  J. Salgado,et al.  Potential of Red, Green and Brown Seaweeds as Substrates for Solid State Fermentation to Increase Their Nutritional Value and to Produce Enzymes , 2022, Foods.

[10]  I. Oey,et al.  Changes in volatile and fatty acid compositions of selected microalgal suspensions with high pressure homogenization , 2022, Algal Research.

[11]  Tao Lu,et al.  Competition of Dual Roles of Ionic Liquids during In Situ Transesterification of Wet Algae , 2022, ACS Sustainable Chemistry & Engineering.

[12]  Fengwei Yin,et al.  Efficient Co-production of Docosahexaenoic Acid Oil and Carotenoids in Aurantiochytrium sp. Using a Light Intensity Gradient Strategy , 2022, Applied Biochemistry and Biotechnology.

[13]  Yi Tong Cheah,et al.  Biomass and eicosapentaenoic acid (EPA) production from Amphora sp. under different environmental and nutritional conditions. , 2022, Biotechnology and applied biochemistry.

[14]  Daniel R. Figueiredo,et al.  Algae as Food in Europe: An Overview of Species Diversity and Their Application , 2022, Foods.

[15]  B. Ruyter,et al.  Improved fillet quality in harvest-size Atlantic salmon fed high n-3 canola oil as a DHA-source , 2022, Aquaculture.

[16]  R. Wijffels,et al.  Heterotrophic vs autotrophic production of microalgae: Bringing some light into the everlasting cost controversy , 2022, Algal Research.

[17]  Yanli Zheng,et al.  Reconstitution and Expression of mcy Gene Cluster in The Model Cyanobacterium Synechococcus 7942 Reveals a Roll of MC-LR in Cell Division , 2022, bioRxiv.

[18]  Hui Chen,et al.  Microalgae-Based Green Bio-Manufacturing—How Far From Us , 2022, Frontiers in Microbiology.

[19]  S. Sugihara,et al.  Application of genetic disruption of a Nannochloropsis oceanica cell wall synthesizing gene to n-3 HUFA enrichment of Brachionus plicatilis , 2022, Aquaculture.

[20]  Meilin He,et al.  Low temperature combined with high inoculum density improves alpha-linolenic acid production and biochemical characteristics of Chlamydomonas reinhardtii. , 2022, Bioresource technology.

[21]  Xiaojun Yan,et al.  A simple and efficient strategy for fucoxanthin extraction from the microalga Phaeodactylum tricornutum , 2022, Algal Research.

[22]  E. Molina Grima,et al.  Supercritical fluid extraction and pressurized liquid extraction processes applied to eicosapentaenoic acid-rich polar lipid recovery from the microalga Nannochloropsis sp , 2022, Algal Research.

[23]  M. Salgado-Cervantes,et al.  Supercritical fluid extraction of lipids rich in DHA from Schizochytrium sp. , 2022, The Journal of Supercritical Fluids.

[24]  A. Srivastava,et al.  Genetic engineering of algae , 2022, An Integration of Phycoremediation Processes in Wastewater Treatment.

[25]  N. He,et al.  Production of polyunsaturated fatty acids by Schizochytrium (Aurantiochytrium) spp. , 2021, Biotechnology advances.

[26]  Pratik R. Pawar,et al.  Co-cultivation of Phaeodactylum tricornutum and Aurantiochytrium limacinum for polyunsaturated omega-3 fatty acids production. , 2021, Bioresource technology.

[27]  Chenliu He,et al.  Optimized production and enrichment of α-linolenic acid by Scenedesmus sp. HSJ296 , 2021, Algal Research.

[28]  Y. Duan,et al.  The composition, extraction, analysis, bioactivities, bioavailability and applications in food system of flaxseed (Linum usitatissimum L.) oil: A review , 2021, Trends in Food Science & Technology.

[29]  A. Treydte,et al.  Fatty Acid Contents and Stability of Oyster Nut Oil (Telfairia pedata) Compared to Flaxseed and Sunflower Oil , 2021, International journal of food science.

[30]  A. Oliveira,et al.  Raw Glycerol Based Medium for DHA and Lipids Production, Using the Marine Heterotrophic Microalga Crypthecodinium cohnii , 2021, Processes.

[31]  C. Jacobsen,et al.  UVB exposure stimulates production of vitamin D3 in selected microalgae , 2021 .

[32]  Y. Alparslan,et al.  Effect of different cell disruption methods on lipid yield of Schizochytrium sp. , 2021, Journal of the American Oil Chemists' Society.

[33]  Q. Hu,et al.  Study on the Effect of Spray Drying Process on the Quality of Microalgal Biomass: a Comprehensive Biocomposition Analysis of Spray-Dried S. acuminatus Biomass , 2021, BioEnergy Research.

[34]  V. Oikonomopoulou,et al.  Optimization of green extractions for the recovery of docosahexaenoic acid (DHA) from Crypthecodinium cohnii , 2021 .

[35]  R. Wijffels,et al.  Fucoxanthin and docosahexaenoic acid production by cold-adapted Tisochrysis lutea. , 2021, New biotechnology.

[36]  D. Gong,et al.  Expression and characterization of a novel lipase from Bacillus licheniformis NCU CS-5 for application in enhancing fatty acids flavor release for low-fat cheeses. , 2021, Food chemistry.

[37]  Q. Hu,et al.  Ultrahigh‐cell‐density heterotrophic cultivation of the unicellular green alga Chlorella sorokiniana for biomass production , 2021, Biotechnology and bioengineering.

[38]  Thomas Kiran Marella,et al.  Impact of organic carbon acquisition on growth and functional biomolecule production in diatoms , 2021, Microbial Cell Factories.

[39]  P. Calder,et al.  Beneficial Outcomes of Omega-6 and Omega-3 Polyunsaturated Fatty Acids on Human Health: An Update for 2021 , 2021, Nutrients.

[40]  Hui Chen,et al.  A Novel Method for Non-invasive Estimation of Primary Productivity in Aquatic Ecosystems Using a Chlorophyll Fluorescence-Induced Dynamic Curve , 2021, Frontiers in Microbiology.

[41]  Hui Chen,et al.  Regulatory mechanisms of lipid biosynthesis in microalgae , 2021, Biological reviews of the Cambridge Philosophical Society.

[42]  Chao Ye,et al.  Strategies for enhancing terpenoids accumulation in microalgae , 2021, Applied Microbiology and Biotechnology.

[43]  J. Kavanagh,et al.  Photoautotrophic production of eicosapentaenoic acid , 2021, Critical reviews in biotechnology.

[44]  F. Corellou,et al.  Temperature Acclimation of the Picoalga Ostreococcus tauri Triggers Early Fatty-Acid Variations and Involves a Plastidial ω3-Desaturase , 2021, Frontiers in Plant Science.

[45]  M. Rozalski,et al.  Effects of Omega-3 Polyunsaturated Fatty Acids and Their Metabolites on Haemostasis—Current Perspectives in Cardiovascular Disease , 2021, International journal of molecular sciences.

[46]  Medhat A. Haroun,et al.  The biopolymer ulvan from Ulva fasciata: Extraction towards nanofibers fabrication. , 2021, International journal of biological macromolecules.

[47]  Jingliang Xu,et al.  Recent progress in extraction/transesterification techniques for the recovery of oil from algae biomass , 2021, Biomass Conversion and Biorefinery.

[48]  Despal,et al.  Effect of different altitudes on milk fatty acid and conjugated linoleic acid (CLA) profiles , 2021 .

[49]  Hui Chen,et al.  Time-course effects of Tris(1,3-dichloro-2-propyl) phosphate (TDCPP) on Chlorella pyrenoidosa: Growth inhibition and adaptability mechanisms. , 2021, Journal of hazardous materials.

[50]  A. Lali,et al.  Isolation and optimization of a novel thraustochytrid strain for DHA rich and astaxanthin comprising biomass as aquafeed supplement , 2021, 3 Biotech.

[51]  Mei-zhen Lu,et al.  Sulfonated Sargassum horneri carbon as solid acid catalyst to produce biodiesel via esterification. , 2021, Bioresource technology.

[52]  Debabrata Barik,et al.  Investigation on algae oil extraction from algae Spirogyra by Soxhlet extraction method , 2021 .

[53]  Sanjoy Banerjee,et al.  High Productivity of Eicosapentaenoic Acid and Fucoxanthin by a Marine Diatom Chaetoceros gracilis in a Semi-Continuous Culture , 2020, Frontiers in Bioengineering and Biotechnology.

[54]  M. Özcan,et al.  Influence of refining stages on the physicochemical properties and phytochemicals of canola oil , 2020 .

[55]  J. Umamaheswari,et al.  Outdoor cultivation of Chlorella pyrenoidosa in paddy-soaked wastewater and a feasibility study on biodiesel production from wet algal biomass through in-situ transesterification , 2020 .

[56]  Hui Chen,et al.  Microalgal biofuels in China: The past, progress and prospects , 2020 .

[57]  Meilin He,et al.  Bioremediation of Pyropia-processing wastewater coupled with lipid production using Chlorella sp. , 2020, Bioresource technology.

[58]  S. Bunn,et al.  Polyunsaturated fatty acids in fish tissues more closely resemble algal than terrestrial diet sources , 2020, Hydrobiologia.

[59]  Ismael H. Pérez-Hernández,et al.  Effect of alpha linolenic acid on membrane fluidity and respiration of liver mitochondria in normoglycemic and diabetic Wistar rats , 2020, Journal of Bioenergetics and Biomembranes.

[60]  Jo‐Shu Chang,et al.  Thermal-Fenton mechanism with sonoprocessing for rapid non-catalytic transesterification of microalgal to biofuel production , 2020 .

[61]  P. Schenk,et al.  Development of High‐Level Omega‐3 Eicosapentaenoic Acid (EPA) Production from Phaeodactylum tricornutum , 2020, Journal of phycology.

[62]  Jinyuan Sun,et al.  Strategies for enhancing eicosapentaenoic acid production: From fermentation to metabolic engineering , 2020 .

[63]  Lirong Song,et al.  Automated accelerated solvent extraction method for total lipid analysis of microalgae , 2020 .

[64]  J. Wang,et al.  A review on flocculation as an efficient method to harvest energy microalgae: Mechanisms, performances, influencing factors and perspectives , 2020 .

[65]  M. Borowitzka.  Phycology , 2020, eLS.

[66]  U. Schmid-Staiger,et al.  Coproduction of EPA and Fucoxanthin withP. tricornutum– A Promising Approach for Up‐ and Downstream Processing , 2020, Chemie Ingenieur Technik.

[67]  Shaoyan Wang,et al.  Separation of eicosapentaenoic acid and docosahexaenoic acid by three-zone simulated moving bed chromatography. , 2020, Journal of chromatography. A.

[68]  J. Simal-Gándara,et al.  Scientific Approaches on Extraction, Purification and Stability for the Commercialization of Fucoxanthin Recovered from Brown Algae , 2020, Foods.

[69]  Y. Kaya,et al.  Effect of deep frying process on the quality of the refined oleic/linoleic sunflower seed oil and olive oil , 2020, Journal of Food Science and Technology.

[70]  F. J. Señoráns,et al.  Advanced Extraction of Lipids with DHA from Isochrysis galbana with Enzymatic Pre-Treatment Combined with Pressurized Liquids and Ultrasound Assisted Extractions , 2020, Molecules.

[71]  N. Kallel,et al.  Efficiency of clay materials collected from Ain Jeloula (Central Tunisia) in sunflower oil decolorization , 2020, Euro-Mediterranean Journal for Environmental Integration.

[72]  L. Albino,et al.  Microalgae proteins: production, separation, isolation, quantification, and application in food and feed , 2020, Critical reviews in food science and nutrition.

[73]  D. Miklavčič,et al.  Electroporation as a Solvent-Free Green Technique for Non-Destructive Extraction of Proteins and Lipids From Chlorella vulgaris , 2020, Frontiers in Bioengineering and Biotechnology.

[74]  M. J. González‐Fernández,et al.  Highly concentrated very long-chain PUFA obtainment by Urea complexation methodology , 2020 .

[75]  O. Shido,et al.  Intake of Alpha-Linolenic Acid-Rich Perilla frutescens Leaf Powder Decreases Home Blood Pressure and Serum Oxidized Low-Density Lipoprotein in Japanese Adults , 2020, Molecules.

[76]  H. Pereira,et al.  Isolation, Identification and Biotechnological Applications of a Novel, Robust, Free-living Chlorococcum (Oophila) amblystomatis Strain Isolated from a Local Pond , 2020, Applied Sciences.

[77]  Bi-hong Hong,et al.  Separation of saturated fatty acids from docosahexaenoic acid‐rich algal oil by enzymatic ethanolysis in tandem with molecular distillation , 2020, Food science & nutrition.

[78]  L. Tavares,et al.  Determination of optimum conditions for thermal regeneration and characterization of a spent bleaching earth , 2020 .

[79]  F. J. Señoráns,et al.  Enzymatic modification to produce health-promoting lipids from fish oil, algae and other new omega-3 sources: a review. , 2020, New biotechnology.

[80]  Qiang Wang,et al.  Microalgae-based nitrogen bioremediation , 2020 .

[81]  Changwei Hu,et al.  Production of high-quality biofuel via ethanol liquefaction of pretreated natural microalgae , 2020 .

[82]  Qingzhe Jin,et al.  Preparation of DHA-Rich Medium- and Long-Chain Triacylglycerols by Lipase-Catalyzed Acidolysis of Microbial Oil from Schizochytrium sp.with Medium-Chain Fatty Acids , 2020, Applied Biochemistry and Biotechnology.

[83]  X. Arakaki,et al.  Polyunsaturated Fatty Acid Composition of Cerebrospinal Fluid Fractions Shows Their Contribution to Cognitive Resilience of a Pre-symptomatic Alzheimer’s Disease Cohort , 2020, Frontiers in Physiology.

[84]  M. Islam,et al.  Separation and Purification of ω-6 Linoleic Acid from Crude Tall Oil , 2020, Separations.

[85]  S. Chianese,et al.  Recent developments in supercritical fluid extraction of bioactive compounds from microalgae: Role of key parameters, technological achievements and challenges , 2020 .

[86]  S. Sim,et al.  Repeated-batch production of omega-3 enriched biomass of Chlorella sorokiniana via calcium-induced homeoviscous adaptation. , 2020, Bioresource technology.

[87]  G. Díaz,et al.  Effect of full-fat sunflower or flaxseed seeds dietary inclusion on performance, egg yolk fatty acid profile and egg quality in laying hens , 2020, Animal nutrition.

[88]  Daniel B. Müller,et al.  Systems approach to quantify the global omega-3 fatty acid cycle , 2020, Nature Food.

[89]  Jithu Paul Jacob.  Bioconcentration of Marine Algae Using Lipase Enzyme , 2019, Microalgae - From Physiology to Application.

[90]  B. Liu,et al.  Novel insights into mixotrophic cultivation of Nitzschia laevis for co-production of fucoxanthin and eicosapentaenoic acid. , 2019, Bioresource technology.

[91]  Q. Hu,et al.  Ultrahigh‐cell‐density heterotrophic cultivation of the unicellular green microalga Scenedesmus acuminatus and application of the cells to photoautotrophic culture enhance biomass and lipid production , 2019, Biotechnology and bioengineering.

[92]  Lifang Wu,et al.  Harnessing the Lipogenic Potential of Δ6-Desaturase for Simultaneous Hyperaccumulation of Lipids and Polyunsaturated Fatty Acids in Nannochloropsis oceanica , 2019, Front. Mar. Sci..

[93]  B. Liu,et al.  Extraction and purification of eicosapentaenoic acid and docosahexaenoic acid from microalgae: A critical review , 2019, Algal Research.

[94]  A. Komansilan,et al.  Enrichment of omega-3 fatty acids, waste oil by-products canning tuna (thunnus sp.) with urea crystallization , 2019, Journal of Physics: Conference Series.

[95]  J. Gonzalez,et al.  Fatty Acid Composition, Proximate Analysis, and Consumer Sensory Evaluation of United States Retail Grass-Fed Ground Beef , 2019, Meat and Muscle Biology.

[96]  H. Nayebzadeh,et al.  Application of magnetic alumina-ferric oxide nanocatalyst supported by KOH for in-situ transesterification of microalgae cultivated in wastewater medium , 2019, Biomass and Bioenergy.

[97]  Y. Chisti,et al.  Mixotrophic production of polyunsaturated fatty acids and carotenoids by the microalga Nannochloropsis gaditana , 2019, Journal of Applied Phycology.

[98]  H. Hara,et al.  Production of α-linolenic Acid by an Oleaginous Green Algae Acutodesmus obliquus Isolated from Malaysia , 2019, Journal of Pure and Applied Microbiology.

[99]  H. Dahms,et al.  Passive cell disruption lipid extraction methods of microalgae for biofuel production – A review , 2019, Fuel.

[100]  Charles B. Felix,et al.  Non-catalytic in-situ (trans) esterification of lipids in wet microalgae Chlorella vulgaris under subcritical conditions for the synthesis of fatty acid methyl esters , 2019, Applied Energy.

[101]  Qian Wang,et al.  Microalgae harvest influences the energy recovery: A case study on chemical flocculation of Scenedesmus obliquus for biodiesel and crude bio-oil production. , 2019, Bioresource technology.

[102]  A. Incharoensakdi,et al.  Enhancement of lipid extraction for efficient methyl ester production from Chlamydomonas sp. , 2019, Journal of Applied Phycology.

[103]  Meng Yang,et al.  Structural Alteration of Montmorillonite by Acid Activation and its Effect on the Decolorization of Rapeseed Oil , 2019, JOM.

[104]  R. Rezaee,et al.  Alpha‐linolenic acid ameliorates bronchial asthma features in ovalbumin‐sensitized rats , 2019, The Journal of pharmacy and pharmacology.

[105]  J. Wan,et al.  n-3 Polyunsaturated fatty acids for the management of alcoholic liver disease: A critical review , 2019, Critical reviews in food science and nutrition.

[106]  Yongjin He,et al.  Storage carbon metabolism of Isochrysis zhangjiangensis under different light intensities and its application for co-production of fucoxanthin and stearidonic acid. , 2019, Bioresource technology.

[107]  L. Medina-Juárez,et al.  Effect of frying and storage on oxidative quality of conjugated linoleic-acid-rich soybean oil produced by photoisomerization using plantain as a model system. , 2019, Journal of the science of food and agriculture.

[108]  A. Solovchenko,et al.  Long-Chain Polyunsaturated Fatty Acids in the Green Microalga Lobosphaera incisa Contribute to Tolerance to Abiotic Stresses. , 2019, Plant & cell physiology.

[109]  S. Aubourg,et al.  Concentration of EPA and DHA from Refined Salmon Oil by Optimizing the Urea–Fatty Acid Adduction Reaction Conditions Using Response Surface Methodology , 2019, Molecules.

[110]  Q. Hu,et al.  Solid Matrix-Supported Supercritical CO2 Enhances Extraction of γ-Linolenic Acid from the Cyanobacterium Arthrospira (Spirulina) platensis and Bioactivity Evaluation of the Molecule in Zebrafish , 2019, Marine drugs.

[111]  Mieczysław Dutka,et al.  Self-regulation of the inflammatory response by peroxisome proliferator-activated receptors , 2019, Inflammation Research.

[112]  Tushar Ranjan Moharana,et al.  Substrate structure and computation guided engineering of a lipase for omega-3 fatty acid selectivity , 2019, bioRxiv.

[113]  M. Chauton,et al.  Influence of Nitrogen Limitation on Lipid Accumulation and EPA and DHA Content in Four Marine Microalgae for Possible Use in Aquafeed , 2019, Front. Mar. Sci..

[114]  D. Boldor,et al.  Central composite design parameterization of microalgae/cyanobacteria co-culture pretreatment for enhanced lipid extraction using an external clamp-on ultrasonic transducer. , 2019, Ultrasonics sonochemistry.

[115]  G. Rorrer,et al.  Phosphate addition strategies for enhancing the co-production of lipid and chitin nanofibers during fed-batch cultivation of the diatom Cyclotella sp. , 2019, Algal Research.

[116]  M. Boskabady,et al.  The effect of alpha linolenic acid on tracheal responsiveness, lung inflammation, and immune markers in sensitized rats , 2019, Iranian journal of basic medical sciences.

[117]  L. Polito,et al.  Hexadecenoic Fatty Acid Positional Isomers and De Novo PUFA Synthesis in Colon Cancer Cells , 2019, International journal of molecular sciences.

[118]  J. Zhan,et al.  Ammonium Nitrogen Tolerant Chlorella Strain Screening and Its Damaging Effects on Photosynthesis , 2019, Front. Microbiol..

[119]  A. Zarli.  Oleochemicals: all time players of green chemistry , 2019 .

[120]  K. Ikeda,et al.  Reelin deficiency leads to aberrant lipid composition in mouse brain. , 2018, Biochemical and biophysical research communications.

[121]  S. Nagappan,et al.  Co-production of biodiesel and alpha-linolenic acid (omega-3 fatty acid) from microalgae, Desmodesmus sp. MCC34 , 2018, Energy Sources, Part A: Recovery, Utilization, and Environmental Effects.

[122]  Yonghua Wang,et al.  Production and immobilization of lipase PCL and its application in synthesis of α-linolenic acid-rich diacylglycerol , 2018, Journal of Food Biochemistry.

[123]  S. Sokhansanj,et al.  Studying the drying mechanism of microalgae Chlorella vulgaris and the optimum drying temperature to preserve quality characteristics , 2018 .

[124]  Zhiyuan Dai,et al.  Enrichment of polyunsaturated fatty acids from seal oil through urea adduction and the fatty acids change rules during the process , 2018 .

[125]  G. Narayanan,et al.  The Role of Polymer Crystallizability on the Formation of Polymer-Urea-Inclusion Compounds , 2018 .

[126]  T. Selvaratnam,et al.  Binary culture of microalgae as an integrated approach for enhanced biomass and metabolites productivity, wastewater treatment, and bioflocculation. , 2018, Chemosphere.

[127]  Tao Wu,et al.  Differential responses of the green microalga Chlorella zofingiensis to the starvation of various nutrients for oil and astaxanthin production. , 2018, Bioresource technology.

[128]  Tianpei Li,et al.  Ten years of algal biofuel and bioproducts: gains and pains , 2018, Planta.

[129]  Matthew D. Johnson,et al.  Marine Cryptophytes Are Great Sources of EPA and DHA , 2017, Marine drugs.

[130]  J. Karimi-Sabet,et al.  Biodiesel production from Spirulina microalgae feedstock using direct transesterification near supercritical methanol condition. , 2017, Bioresource technology.

[131]  A. Eidi,et al.  The Extract of Portulaca oleracea and Its Constituent, Alpha Linolenic Acid Affects Serum Oxidant Levels and Inflammatory Cells in Sensitized Rats. , 2017, Iranian journal of allergy, asthma, and immunology.

[132]  K. Sigler,et al.  Polydatin and its derivatives inhibit fatty acid desaturases in microorganisms , 2017 .

[133]  D. Copolovici,et al.  The Fatty Acids Composition and Antioxidant Activity of Walnut Cold Press Oil , 2017 .

[134]  Y. Chang,et al.  Wet in situ transesterification of microalgae using ethyl acetate as a co-solvent and reactant. , 2017, Bioresource technology.

[135]  S. Mathew,et al.  Effect of Lipases from Candida cylinderacea on Enrichment of PUFA in Marine Microalgae , 2017 .

[136]  K. Best,et al.  Polyunsaturated Fatty Acids: Metabolism and Nutritional Requirements in Pregnancy and Infancy , 2017 .

[137]  H. Aukema,et al.  Omega-3 Polyunsaturated Fatty Acids and Oxylipins in Neuroinflammation and Management of Alzheimer Disease. , 2016, Advances in nutrition.

[138]  M. Guarnieri,et al.  Simultaneous production of triacylglycerol and high-value carotenoids by the astaxanthin-producing oleaginous green microalga Chlorella zofingiensis. , 2016, Bioresource technology.

[139]  Qiang Wang,et al.  Improved Productivity of Neutral Lipids in Chlorella sp. A2 by Minimal Nitrogen Supply , 2016, Front. Microbiol..

[140]  Jin Liu,et al.  Lipid Production from Nannochloropsis , 2016, Marine drugs.

[141]  Junfeng Rong,et al.  Effective Biological DeNOx of Industrial Flue Gas by the Mixotrophic Cultivation of an Oil-Producing Green Alga Chlorella sp. C2. , 2016, Environmental science & technology.

[142]  J. Linnekoski,et al.  Conversion of polar and non-polar algae oil lipids to fatty acid methyl esters with solid acid catalysts--A model compound study. , 2015, Bioresource technology.

[143]  Vikas Singh Chauhan,et al.  Characterization of fatty acids and hydrocarbons of chlorophycean microalgae towards their use as biofuel source , 2015 .

[144]  Bora Kim,et al.  In situ transesterification of highly wet microalgae using hydrochloric acid. , 2015, Bioresource technology.

[145]  L. Lagrost,et al.  Liver X Receptor Activation Promotes Polyunsaturated Fatty Acid Synthesis in Macrophages: Relevance in the Context of Atherosclerosis , 2015, Arteriosclerosis, thrombosis, and vascular biology.

[146]  J. Pruvost,et al.  A novel recovery process for lipids from microalgæ for biodiesel production using a hydrated phosphonium ionic liquid , 2015 .

[147]  Y. Oh,et al.  Breaking dormancy: an energy-efficient means of recovering astaxanthin from microalgae , 2015 .

[148]  M. Barzegar,et al.  Influence of processing parameters on physicochemical properties of fractionated fish oil at low temperature crystallization , 2015 .

[149]  D. Xie,et al.  Sustainable source of omega-3 eicosapentaenoic acid from metabolically engineered Yarrowia lipolytica: from fundamental research to commercial production , 2015, Applied Microbiology and Biotechnology.

[150]  Esther S. Kim,et al.  Icosapent Ethyl: A Review of Its Use in Severe Hypertriglyceridemia , 2014, American Journal of Cardiovascular Drugs.

[151]  F. Kreps,et al.  Influence of industrial physical refining on tocopherol, chlorophyll and beta‐carotene content in sunflower and rapeseed oil , 2014 .

[152]  Ani Idris,et al.  Enhancing the various solvent extraction method via microwave irradiation for extraction of lipids from marine microalgae in biodiesel production. , 2014, Bioresource technology.

[153]  A. R. Medina,et al.  Concentration of docosahexaenoic and eicosapentaenoic acids by enzymatic alcoholysis with different acyl-acceptors , 2014 .

[154]  J. Weiss,et al.  Influence of surfactant composition on physical and oxidative stability of Quillaja saponin-stabilized lipid particles with encapsulated ω-3 fish oil. , 2014, Colloids and surfaces. B, Biointerfaces.

[155]  Sohita Dhillon,et al.  Omega-3 Carboxylic Acids (Epanova®): A Review of Its Use in Patients with Severe Hypertriglyceridemia , 2014, American Journal of Cardiovascular Drugs.

[156]  B. Nasernejad,et al.  Pretreatment and kinetics of oil extraction from algae for biodiesel production , 2014 .

[157]  Sung Bae Kim,et al.  Optimization of decoloring conditions of crude fatty acids recovered from crude glycerol by acid-activated clay using response surface method , 2014, Korean Journal of Chemical Engineering.

[158]  Alberto Bertucco,et al.  Exploiting microalgae as a source of essential fatty acids by supercritical fluid extraction of lipids: Comparison between Scenedesmus obliquus, Chlorella protothecoides and Nannochloropsis salina , 2014 .

[159]  S. Rajaram.  Health benefits of plant-derived α-linolenic acid. , 2014, The American journal of clinical nutrition.

[160]  Duo Li,et al.  Ratio of n-3/n-6 PUFAs and risk of breast cancer: a meta-analysis of 274135 adult females from 11 independent prospective studies , 2014, BMC Cancer.

[161]  You-Kwan Oh,et al.  Acid-catalyzed hot-water extraction of lipids from Chlorella vulgaris. , 2014, Bioresource technology.

[162]  F. Bux,et al.  An evaluation of the efficacy of using selected solvents for the extraction of lipids from algal biomass by the soxhlet extraction method , 2014 .

[163]  C. Frances,et al.  Extraction of lipids and pigments of Chlorella vulgaris by supercritical carbon dioxide: influence of bead milling on extraction performance , 2014, Journal of Applied Phycology.

[164]  Duo Li,et al.  Ratio of n-3/n-6 PUFAs and risk of breast cancer: a meta-analysis of 274135 adult females from 11 independent prospective studies , 2014, BMC Cancer.

[165]  D. Stengel,et al.  Fatty acid contents and profiles of 16 macroalgae collected from the Irish Coast at two seasons , 2014, Journal of Applied Phycology.

[166]  A. R. Medina,et al.  Concentration of eicosapentaenoic acid (EPA) by selective alcoholysis catalyzed by lipases , 2013 .

[167]  T. Lüscher,et al.  Dietary α-linolenic acid increases the platelet count in ApoE-/- mice by reducing clearance. , 2013, Blood.

[168]  Seung-Pyo Hong,et al.  Production of omega-3 eicosapentaenoic acid by metabolic engineering of Yarrowia lipolytica , 2013, Nature Biotechnology.

[169]  Fuan Wu,et al.  Enrichment process for α‐linolenic acid from silkworm pupae oil , 2013 .

[170]  Q. Hu,et al.  Astaxanthin in microalgae: pathways, functions and biotechnological implications , 2013 .

[171]  Varun,et al.  Performance and emission characteristics of biodiesel from different origins: A review , 2013 .

[172]  C. Barrow,et al.  Selective concentration of EPA and DHA using Thermomyces lanuginosus lipase is due to fatty acid selectivity and not regioselectivity. , 2013, Food chemistry.

[173]  Yuanfa Liu,et al.  Concentration of Omega-3 Polyunsaturated Fatty Acids from Oil of Schizochytrium limacinum by Molecular Distillation: Optimization of Technological Conditions , 2013 .

[174]  Chen Guo,et al.  Algal oil extraction from wet biomass of Botryococcus braunii by 1,2-dimethoxyethane , 2013 .

[175]  M. Borowitzka.  High-value products from microalgae—their development and commercialisation , 2013, Journal of Applied Phycology.

[176]  M. Borowitzka.  High-value products from microalgae—their development and commercialisation , 2013, Journal of Applied Phycology.

[177]  G. Ciudad,et al.  Advances in direct transesterification of microalgal biomass for biodiesel production , 2013, Reviews in Environmental Science and Bio/Technology.

[178]  G. Najafpour,et al.  Lipase-mediated hydrolysis of flax seed oil for selective enrichment of α-linolenic acid , 2012 .

[179]  Li Xu,et al.  Efficient display of active Geotrichum sp. lipase on Pichia pastoris cell wall and its application as a whole-cell biocatalyst to enrich EPA and DHA in fish oil. , 2012, Journal of agricultural and food chemistry.

[180]  P. Schenk,et al.  Microalgal biofactories: a promising approach towards sustainable omega-3 fatty acid production , 2012, Microbial Cell Factories.

[181]  Junhai Liu,et al.  Microwave-assisted direct liquefaction of Ulva prolifera for bio-oil production by acid catalysis. , 2012, Bioresource technology.

[182]  Srinivasa Reddy Ronda,et al.  Aeration effect on Spirulina platensis growth and γ-Linolenic acid production , 2012, Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology].

[183]  H. Uemura.  Synthesis and production of unsaturated and polyunsaturated fatty acids in yeast: current state and perspectives , 2012, Applied Microbiology and Biotechnology.

[184]  Wei Zhang,et al.  Biodiesel production from algae oil high in free fatty acids by two-step catalytic conversion. , 2012, Bioresource technology.

[185]  Razif Harun,et al.  Microalgal cell disruption for biofuel development , 2012 .

[186]  L. A. Pinto,et al.  Polyunsaturated Fatty Acid Concentrates of Carp Oil: Chemical Hydrolysis and Urea Complexation , 2012 .

[187]  W. Gerrits,et al.  Linoleic and α-linolenic acid as precursor and inhibitor for the synthesis of long-chain polyunsaturated fatty acids in liver and brain of growing pigs. , 2012, Animal : an international journal of animal bioscience.

[188]  V. Strezov,et al.  Assessment of Bio-oil Extraction from Tetraselmis chui Microalgae Comparing Supercritical CO2, Solvent Extraction, and Thermal Processing , 2012 .

[189]  Sheridan M. Hoy,et al.  Omega-3 Ethylester Concentrate , 2009, Drugs.

[190]  Danielle Swanson,et al.  Omega-3 fatty acids EPA and DHA: health benefits throughout life. , 2012, Advances in nutrition.

[191]  Farid Chemat,et al.  Combined Extraction Processes of Lipid from Chlorella vulgaris Microalgae: Microwave Prior to Supercritical Carbon Dioxide Extraction , 2011, International journal of molecular sciences.

[192]  Guangrui Liu,et al.  Study on the Decolorization of Biodiesel from Waste Cooking Oil , 2011 .

[193]  F. Bux,et al.  Dual role of microalgae: Phycoremediation of domestic wastewater and biomass production for sustainable biofuels production , 2011 .

[194]  Howland D. T. Jones,et al.  Carotenoid Distribution in Living Cells of Haematococcus pluvialis (Chlorophyceae) , 2011, PloS one.

[195]  B. Berra,et al.  Effects of n-3 PUFAs on breast cancer cells through their incorporation in plasma membrane , 2011, Lipids in Health and Disease.

[196]  Roberto E. Armenta,et al.  Developments in oil extraction from microalgae. , 2011 .

[197]  M. D. D. do Carmo,et al.  Effects of maternal malnutrition and postnatal nutritional rehabilitation on brain fatty acids, learning, and memory. , 2011, Nutrition reviews.

[198]  Xiao-Jun Ji,et al.  Disruption of Chlorella vulgaris Cells for the Release of Biodiesel-Producing Lipids: A Comparison of Grinding, Ultrasonication, Bead Milling, Enzymatic Lysis, and Microwaves , 2011, Applied biochemistry and biotechnology.

[199]  B. Becher,et al.  Dietary α-linolenic acid diminishes experimental atherogenesis and restricts T cell-driven inflammation , 2011, European heart journal.

[200]  W. Zhao-jun.  Optimization of the Enrichment of Alpha-Linolenic Acid in Silkworm Pupal Oil by the Method of Urea Adduction Fractionation , 2011 .

[201]  A. S. Babji,et al.  Extraction of lipids and purification of linoleic acid from Clarias macrocephalus oil , 2011 .

[202]  P. Guesnet,et al.  Docosahexaenoic acid (DHA) and the developing central nervous system (CNS) - Implications for dietary recommendations. , 2011, Biochimie.

[203]  A. Quiroz,et al.  Docosahexaenoic acid (C22:6n-3, DHA) and astaxanthin production by Thraustochytriidae sp. AS4-A1 a native strain with high similitude to Ulkenia sp.: evaluation of liquid residues from food industry as nutrient sources. , 2010 .

[204]  H. Yatsuya,et al.  Higher dietary intake of alpha-linolenic acid is associated with lower insulin resistance in middle-aged Japanese. , 2010, Preventive medicine.

[205]  Vijayanand S. Moholkar,et al.  Mechanistic Assessment of Microalgal Lipid Extraction , 2010 .

[206]  Philip Owende,et al.  Biofuels from microalgae—A review of technologies for production, processing, and extractions of biofuels and co-products , 2010 .

[207]  H. Oh,et al.  Comparison of several methods for effective lipid extraction from microalgae. , 2010, Bioresource technology.

[208]  Michael J. Cooney,et al.  Extraction of Bio‐oils from Microalgae , 2009 .

[209]  G. Knothe,et al.  A Comprehensive Evaluation of the Melting Points of Fatty Acids and Esters Determined by Differential Scanning Calorimetry , 2009 .

[210]  S. Cunnane,et al.  alpha-Linolenic acid supplementation and conversion to n-3 long-chain polyunsaturated fatty acids in humans. , 2009, Prostaglandins, leukotrienes, and essential fatty acids.

[211]  W. Banks,et al.  Effect of dietary n-3 polyunsaturated fatty acids on brain lipid fatty acid composition, learning ability, and memory of senescence-accelerated mouse. , 2008, The journals of gerontology. Series A, Biological sciences and medical sciences.

[212]  C. Ratledge.  Chapter 4. Microbial Lipids , 2008 .

[213]  Wolfgang Becker,et al.  Microalgae in human and animal nutrition. , 2007 .

[214]  W. Becker.  Microalgae for aquaculture: the nutritional value of microalgae for aquaculture. , 2007 .

[215]  D. Panagiotakos,et al.  Dietary supplementation with flaxseed oil lowers blood pressure in dyslipidaemic patients , 2007, European Journal of Clinical Nutrition.

[216]  A. Madadlou,et al.  Trans-free Iranian vanaspati through enzymatic and chemical transesterification of triple blends of fully hydrogenated soybean, rapeseed and sunflower oils , 2007 .

[217]  J. Navarro,et al.  Dietary deficiency of docosahexaenoic acid impairs vision at low light intensities in juvenile herring (Clupea harengus L.) , 1995, Lipids.

[218]  C. Parrish,et al.  Cholestane as a digestibility marker in the absorption of polyunsaturated fatty acid ethyl esters in Atlantic salmon , 1992, Lipids.

[219]  Owen P. Ward,et al.  Omega-3/6 fatty acids: Alternative sources of production , 2005 .

[220]  J. Giddings,et al.  Varying the ratio of dietary n-6/n-3 polyunsaturated fatty acid alters the tendency to thrombosis and progress of atherosclerosis in apoE-/- LDLR-/- double knockout mouse. , 2005, Thrombosis research.

[221]  Han Xi-jiang.  Preparation of High-Purity Linolenic Acid from Oil of Lithospermum Erythrorhizon by Urea Inclusion and Column Chromatography , 2004 .

[222]  D. Nichols.  Prokaryotes and the input of polyunsaturated fatty acids to the marine food web. , 2003, FEMS microbiology letters.

[223]  Lawrence J Appel,et al.  Fish consumption, fish oil, omega-3 fatty acids, and cardiovascular disease. , 2002, Arteriosclerosis, thrombosis, and vascular biology.

[224]  Frank D. Gunstone,et al.  Vegetable Oils in Food Technology: Composition, Properties, and Uses , 2002 .

[225]  B. Jeffrey,et al.  Visual acuity and retinal function in infant monkeys fed long-chain PUFA , 2002, Lipids.

[226]  P. Calder,et al.  Polyunsaturated fatty acids, inflammation, and immunity , 2001, Lipids.

[227]  E. Zrenner,et al.  Changes of kynurenic acid content in the rat and chicken retina during ontogeny , 2002, Graefe's Archive for Clinical and Experimental Ophthalmology.

[228]  J. Napier.  Plumbing the depths of PUFA biosynthesis: a novel polyketide synthase-like pathway from marine organisms. , 2002, Trends in plant science.

[229]  G. Jensen,et al.  Blue-Green Algae as an Immuno-Enhancer and Biomodulator , 2001 .

[230]  F. Shahidi,et al.  CONCENTRATION OF GAMMA LINOLENIC ACID (GLA) FROM BORAGE OIL BY UREA COMPLEXATION: OPTIMIZATION OF REACTION CONDITIONS , 2000 .

[231]  A. Leaf,et al.  Workshop statement on the essentiality of and recommended dietary intakes for Omega-6 and Omega-3 fatty acids. , 2000, Prostaglandins, leukotrienes, and essential fatty acids.

[232]  J. Joseph,et al.  Essential fatty acids and the brain: possible health implications , 2000, International Journal of Developmental Neuroscience.

[233]  R. Gibson,et al.  Dietary polyunsaturated fatty acids and inflammatory mediator production. , 2000, The American journal of clinical nutrition.

[234]  A. Vingrys,et al.  The effects of dietary α-linolenic acid compared with docosahexaenoic acid on brain, retina, liver, and heart in the guinea pig , 1999, Lipids.

[235]  H. Tojo,et al.  High-linoleate and high-alpha-linolenate diets affect learning ability and natural behavior in SAMR1 mice. , 1999, The Journal of nutrition.

[236]  Michael T. Brett,et al.  The role of highly unsaturated fatty acids in aquatic foodweb processes , 1997 .

[237]  A. C. Hillier,et al.  Superstructure Control in the Crystal Growth and Ordering of Urea Inclusion Compounds , 1996, Science.

[238]  R. Ayerza.  Oil content and fatty acid composition of chia (Salvia hispanica L.) from five northwestern locations in Argentina , 1995 .

[239]  N. Raper,et al.  Omega-3 fatty acid content of the US food supply. , 1992, Journal of the American College of Nutrition.

[240]  A. Simopoulos.  Omega-3 fatty acids in health and disease and in growth and development. , 1991, The American journal of clinical nutrition.

[241]  A. Kuksis,et al.  Lumenal hydrolysis of menhaden and rapeseed oils and their fatty acid methyl and ethyl esters in the rat. , 1989, Biochemistry and cell biology = Biochimie et biologie cellulaire.

[242]  D. S. Lin,et al.  Biochemical and functional effects of prenatal and postnatal omega 3 fatty acid deficiency on retina and brain in rhesus monkeys. , 1986, Proceedings of the National Academy of Sciences of the United States of America.

[243]  Hee-Yong Kim,et al.  CHAPTER 15 – DOCOSAHEXAENOIC ACID: MEMBRANE FUNCTION AND METABOLISM , 1986 .

[244]  W. N. Sumerwell,et al.  The fractionation of marine-oil fatty acids with urea , 1955 .

[245]  F. Collins,et al.  Principles of Biochemistry , 1937, The Indian Medical Gazette.