Ultraviolet Detection of Monosaccharides: Multiple Wavelength Strategy to Evaluate Results after Capillary Zone Electrophoretic Separation

Efficient overall processing of wood biomass still lacks suitable concepts for analytical strategies. The traditional strategy is based on compound-targeted analytical tools, which, however, tend to expand the range of required methods for each unit process and restrict the amount of results gained from process monitoring. Especially, development of heterogeneous bioprocess streams would benefit from expanding the range of results gained from applied analytical methods. Therefore, this study was executed in order to demonstrate a novel strategy for the analytical methods to meet the requirements of suitable and efficient process monitoring. The advantages of a novel strategy for interpretation of chemical analysis results by exploiting capillary zone electrophoresis (CZE) with ultraviolet (UV) detection at two complementary wavelengths are presented. Electropherograms of wood-based bioprocess samples were studied as examples with a heterogeneous composition of carbohydrates. Results show that a fairly simple CZE method has the advantage of providing a thorough characterisation of the sample profiles in the preliminary identification of unknown compounds in a heterogeneous wood-based sample.