Transcriptional Analysis of Genes Encoding Shiga Toxin 2 and Its Variants in Escherichia coli

ABSTRACT Six of 37 non-O157 Escherichia coli strains possessing Shiga toxin (Stx) 2 gene variant stx2d or stx2e secreted no detectable Stx. These isolates produced significantly less stx mRNA than Stx2d, Stx2e, Stx2c, or Stx2 secretors did. Standard screening procedures miss a significant subset of E. coli harboring stx2 variants.

[1]  W. Yam,et al.  A Newly Discovered Verotoxin Variant, VT2g, Produced by Bovine Verocytotoxigenic Escherichia coli , 2003, Applied and Environmental Microbiology.

[2]  F. Conraths,et al.  Evaluation of Enzyme-Linked Immunosorbent Assays and a PCR Test for Detection of Shiga Toxins for Shiga Toxin-Producing Escherichia coli in Cattle Herds , 2003, Journal of Clinical Microbiology.

[3]  F. Gunzer,et al.  Comparison of a Shiga Toxin Enzyme-Linked Immunosorbent Assay and Two Types of PCR for Detection of Shiga Toxin-Producing Escherichia coli in Human Stool Specimens , 2003, Journal of Clinical Microbiology.

[4]  Gilbert S Chen,et al.  Shiga toxin-producing Escherichia coli in Montana: bacterial genotypes and clinical profiles. , 2003, The Journal of infectious diseases.

[5]  M. Waldor,et al.  Comparison of Shiga Toxin Production by Hemolytic-Uremic Syndrome-Associated and Bovine-Associated Shiga Toxin-Producing Escherichia coli Isolates , 2003, Applied and Environmental Microbiology.

[6]  M. Ciol,et al.  Escherichia coli O157:H7 infections: discordance between filterable fecal shiga toxin and disease outcome. , 2002, The Journal of infectious diseases.

[7]  A. Friedrich,et al.  Escherichia coli harboring Shiga toxin 2 gene variants: frequency and association with clinical symptoms. , 2002, The Journal of infectious diseases.

[8]  Robert A. LaRossa,et al.  Global Impact of sdiA Amplification Revealed by Comprehensive Gene Expression Profiling of Escherichia coli , 2001, Journal of bacteriology.

[9]  H. Karch,et al.  Characterization of a Shiga Toxin 2e-Converting Bacteriophage from an Escherichia coli Strain of Human Origin , 2000, Infection and Immunity.

[10]  A. Caprioli,et al.  A New Shiga Toxin 2 Variant (Stx2f) fromEscherichia coli Isolated from Pigeons , 2000, Applied and Environmental Microbiology.

[11]  M. Waldor,et al.  Isogenic Lysogens of Diverse Shiga Toxin 2-Encoding Bacteriophages Produce Markedly Different Amounts of Shiga Toxin , 1999, Infection and Immunity.

[12]  K. Shinagawa,et al.  Identification of an insertion sequence, IS1203 variant, in a Shiga toxin 2 gene of Escherichia coli O157:H7. , 1999, Journal of bioscience and bioengineering.

[13]  S. Lauwers,et al.  Identification of New Verocytotoxin Type 2 Variant B-Subunit Genes in Human and Animal Escherichia coliIsolates , 1998, Journal of Clinical Microbiology.

[14]  M. Neely,et al.  Functional and genetic analysis of regulatory regions of coliphage H‐19B: location of shiga‐like toxin and lysis genes suggest a role for phage functions in toxin release , 1998, Molecular microbiology.

[15]  M. Timm,et al.  [A method for specific isolation of verotoxin-producing Escherichia coli colonies]. , 1996, Berliner und Munchener tierarztliche Wochenschrift.

[16]  A. O’Brien,et al.  Activation of Shiga-like toxins by mouse and human intestinal mucus correlates with virulence of enterohemorrhagic Escherichia coli O91:H21 isolates in orally infected, streptomycin-treated mice , 1996, Infection and immunity.

[17]  F. Gunzer,et al.  Construction of recombinant Shiga-like toxin-IIv (SLT-IIv) and its use in monitoring the SLT-IIv antibody status of pigs. , 1995, Veterinary microbiology.

[18]  H. Karch,et al.  Prevalence of attaching and effacing Escherichia coli in stool samples from patients and controls. , 1994, Zentralblatt fur Bakteriologie : international journal of medical microbiology.

[19]  M. McKee,et al.  Two copies of Shiga-like toxin II-related genes common in enterohemorrhagic Escherichia coli strains are responsible for the antigenic heterogeneity of the O157:H- strain E32511 , 1991, Infection and immunity.

[20]  M. P. Jackson,et al.  Cloning and sequencing of a Shiga-like toxin type II variant from Escherichia coli strain responsible for edema disease of swine , 1988, Journal of bacteriology.

[21]  J. Mekalanos,et al.  Evidence that glutamic acid 167 is an active-site residue of Shiga-like toxin I. , 1988, Proceedings of the National Academy of Sciences of the United States of America.

[22]  H. Lior,et al.  The association between idiopathic hemolytic uremic syndrome and infection by verotoxin-producing Escherichia coli. , 1985, The Journal of infectious diseases.