Investigation of the effect of phosphogypsum amendment on two Arabidopsis thaliana ecotype growth and development

The production of phosphoric acid from natural phosphate rock leads to an industrial waste called phosphogypsum (PG). About 5 tons of PG are generated per ton of phosphoric acid produced. This acidic waste (pH 2.2) is mostly disposed of by dumping into large stockpiles close to fertilizer production units, where they occupy large land areas that can cause serious environmental damages. Several attempts were made to test PG valorization via soil amendment because of its phosphate, sulphate and calcium content. The aim of the this study was to evaluate the potential use of PG as phosphate amendment in soil using two wild-type Arabidopsis thaliana ecotypes (Wassilewskija and Colombia) as model plants. Plants were grown in a greenhouse for 30 days, on substrates containing various PG concentrations (0%, 15%, 25%, 40% and 50%). The growth rate and physiological parameters (fresh weight, phosphate and chlorophyll content) were determined. The data revealed that 15% PG did not alter plant survival and leaf's dry weight, and the inorganic phosphate (Pi) uptake by plant seemed to be efficient. However, some alterations in Chlorophyll a/Chlorophyll b ratio were noticed. Higher PG concentrations (40 and 50% PG) exhibited an enhanced negative effect on plant growth, survival and Pi uptake. These inhibitory effects of the substrates may be related to the acidity of the medium in addition to its Cd content.

[1]  H. Marschner.  Mineral Nutrition of Higher Plants , 1988 .

[2]  Markus Puschenreiter,et al.  Cadmium and Zn availability as affected by pH manipulation and its assessment by soil extraction, DGT and indicator plants. , 2012, The Science of the total environment.

[3]  N. Ouaini,et al.  Effects of fertilizer industry emissions on local soil contamination: a case study of a phosphate plant on the east Mediterranean coast , 2012, Environmental technology.

[5]  A. B. Parreira,et al.  Influence of portland cement type on unconfined compressive strength and linear expansion of cement-stabilized phosphogypsum , 2003 .

[6]  M. AL-Oudat,et al.  Case study: heavy metals and fluoride contents in the materials of Syrian phosphate industry and in the vicinity of phosphogypsum piles , 2012, Environmental technology.

[7]  C. C. Mitchell,et al.  Use of phosphogypsum to increase yield and quality of annual forages. , 1990 .

[8]  G. Rayment,et al.  Plant-Soil Interactions at Low pH: Principles and Management , 1995, Developments in Plant and Soil Sciences.

[9]  N. Senesi,et al.  Trace element inputs into soils by anthropogenic activities and implications for human health. , 1999, Chemosphere.

[10]  G. Mackinney,et al.  ABSORPTION OF LIGHT BY CHLOROPHYLL SOLUTIONS , 1941 .

[11]  茅野 充男.  Phytoremediation , 1997, Springer International Publishing.

[12]  S. Machado,et al.  A study of the routes of contamination by lead and cadmium in Santo Amaro, Brazil: A response to the comments of Andrade Lima , 2013, Environmental technology.

[13]  H. R. V. Uexküll,et al.  Global extent, development and economic impact of acid soils , 1995 .

[14]  R. Gadre,et al.  Effect of PEG-6000 imposed water deficit on chlorophyll metabolism in maize leaves , 2009 .

[15]  Y. Poirier,et al.  Regulation of phosphate starvation responses in plants: signaling players and cross-talks. , 2010, Molecular plant.

[16]  F. Zengin,et al.  Toxic effects of cadmium (Cd++) on metabolism of sunflower (Helianthus annuus L.) seedlings , 2006 .

[17]  Aurora López-Delgado,et al.  Environmental impact and management of phosphogypsum. , 2009, Journal of environmental management.

[18]  D. Arnon.  COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS. , 1949, Plant physiology.

[19]  K. Masmoudi,et al.  Phytoremediation potential of Arabidopsis thaliana, expressing ectopically a vacuolar proton pump, for the industrial waste phosphogypsum , 2012, Environmental Science and Pollution Research.

[20]  C. Hermans,et al.  Mechanisms to cope with arsenic or cadmium excess in plants. , 2009, Current opinion in plant biology.

[21]  W. Maksymiec,et al.  Heavy Metal Interactions with Plant Nutrients , 2002 .

[22]  N. Ouaini,et al.  Mobility of selected trace elements in Mediterranean red soil amended with phosphogypsum: experimental study , 2012, Environmental Monitoring and Assessment.

[23]  T. Chiou,et al.  Vacuolar Ca2+/H+ Transport Activity Is Required for Systemic Phosphate Homeostasis Involving Shoot-to-Root Signaling in Arabidopsis1[W][OA] , 2011, Plant Physiology.

[24]  E. Silva,et al.  Levels of selected potential harmful elements (PHEs) in soils and vegetables used in diet of the population living in the surroundings of the Estarreja Chemical Complex (Portugal) , 2014 .

[25]  F. Bingham,et al.  Influence of Calcium and Magnesium Salts on Acid Soil Chemistry and Calcium Nutrition of Apple , 1987 .

[26]  E. Nada,et al.  Cadmium-induced growth inhibition and alteration of biochemical parameters in almond seedlings grown in solution culture , 2007, Acta Physiologiae Plantarum.

[27]  A. Parida,et al.  Effects of NaCl Stress on the Structure, Pigment Complex Composition, and Photosynthetic Activity of Mangrove Bruguiera parviflora Chloroplasts , 2003, Photosynthetica.

[28]  W. M. Stewart,et al.  Phosphorus as a Natural Resource , 2015 .

[29]  Yafeng Wang,et al.  Metal-resistant microorganisms and metal chelators synergistically enhance the phytoremediation efficiency of Solanum nigrum L. in Cd- and Pb-contaminated soil , 2012, Environmental technology.

[30]  S. Safferman,et al.  Evaluation of the bioremediation of a contaminated soil with phytotoxicity tests , 1993 .

[31]  Y. Kamiya,et al.  Effects of heavy metals on seed germination and early seedling growth of Arabidopsis thaliana , 2005, Plant Growth Regulation.

[32]  E. Delhaize,et al.  Characterization of a Phosphate-Accumulator Mutant of Arabidopsis thaliana , 1995, Plant Physiology.

[33]  K. Masmoudi,et al.  Transgenic tobacco plants expressing ectopically wheat H⁺-pyrophosphatase (H⁺-PPase) gene TaVP1 show enhanced accumulation and tolerance to cadmium. , 2012, Journal of plant physiology.

[34]  P. Das,et al.  Studies on cadmium toxicity in plants: a review. , 1997, Environmental pollution.

[35]  K. Hirschi,et al.  Enhancing tonoplast Cd/H antiport activity increases Cd, Zn, and Mn tolerance, and impacts root/shoot Cd partitioning in Nicotiana tabacum L. , 2007, Planta.

[36]  F. W. Smith,et al.  Phosphate transport in plants , 2004, Plant and Soil.

[37]  Andrew N. Sharpley,et al.  Phosphorus: Agriculture and the Environment , 2005 .

[38]  B. Lahner,et al.  Arabidopsis pdr2 reveals a phosphate-sensitive checkpoint in root development. , 2004, The Plant journal : for cell and molecular biology.

[39]  L. D. Norton,et al.  EFFECTS OF SURFACE TREATMENT ON SURFACE SEALING, RUNOFF, AND INTERRILL EROSION , 1998 .

[40]  H. Zimmer,et al.  Growing up or growing out? How soil pH and light affect seedling growth of a relictual rainforest tree , 2014, AoB PLANTS.

[41]  L. Reale,et al.  Responses induced by high concentration of cadmium in Phragmites australis roots. , 2004, Physiologia plantarum.

[42]  Y. M. Amin,et al.  Distribution of some trace metals in Syrian phosphogypsum , 2004 .

[43]  M. Prasad,et al.  Cadmium toxicity and tolerance in vascular plants , 1995 .

[44]  J. Arocena,et al.  Heterogeneous distribution of trace elements and fluorine in phosphogypsum by-product , 1995 .

[45]  B. Balen,et al.  The Effects of Cadmium-Zinc Interactions on Biochemical Responses in Tobacco Seedlings and Adult Plants , 2014, PloS one.

[46]  E. Mutert,et al.  Global extent, development and economic impact of acid soils , 1995, Plant and Soil.

[47]  A. V. Mane,et al.  Salinity induced changes in photosynthetic pigments and polyphenols of Cymbopogon Nardus (L.) Rendle. , 2010 .

[48]  J. Gray,et al.  Differential adaptation of two varieties of common bean to abiotic stress: II. Acclimation of photosynthesis. , 2006, Journal of experimental botany.

[49]  Antonio Delgado,et al.  Fertilizer Phosphorus Recovery from Gypsum-Amended, Reclaimed Calcareous Marsh Soils , 2002 .

[50]  S. Machado,et al.  A study of the routes of contamination by lead and cadmium in Santo Amaro, Brazil , 2013, Environmental technology.

[51]  M. Prasad,et al.  Physiology and Biochemistry of Metal Toxicity and Tolerance in Plants , 2002, Springer Netherlands.

[52]  Tao Chen,et al.  Heavy metal pollution in soils from abandoned Taizhou Chemical Industry Zone in Zhejiang province , 2015, Environmental technology.

[53]  G. Jilani,et al.  Calcium invigorates the cadmium-stressed Brassica napus L. plants by strengthening their photosynthetic system , 2011, Environmental science and pollution research international.