A beta-lactamase belonging to group 2e from oral clinical isolates of Prevotella intermedia.

A beta-lactamase in oral clinical isolates of Prevotella intermedia that hydrolyzed cefuroxime and cephalothin with rates of 600 and 53.3 respectively, relative to that for cephaloridine (100), was characterized as a 2e-cephalosporinase. Inhibition was observed by clavulanic acid (IC50 0.72 microM), tazobactam (IC50 0.21 microM) and sulbactam (IC50 0.07 microM) and was not inhibited by cloxacillin, EDTA, NaCl or p-CMB. The pI and pH optima were 4.7 and 5.4, respectively.

[1]  T. F. Walsh,et al.  Characterisation of black-pigmented anaerobes isolated from diseased and healthy periodontal sites. , 1995, Journal of periodontal research.

[2]  G. Jacoby,et al.  A functional classification scheme for beta-lactamases and its correlation with molecular structure , 1995, Antimicrobial agents and chemotherapy.

[3]  J. Bartlett.  Anaerobic bacterial infections of the lung and pleural space. , 1993, Clinical infectious diseases : an official publication of the Infectious Diseases Society of America.

[4]  C. C. Johnson,et al.  Susceptibility of Anaerobic Bacteria to β-Lactam Antibiotics in the United States , 1993 .

[5]  S. Finegold,et al.  Bacteriology of anaerobic pleuropulmonary infections: preliminary report. , 1993, Clinical infectious diseases : an official publication of the Infectious Diseases Society of America.

[6]  K. Bush,et al.  Antimicrobial resistance in Bacteroides. , 1993, Clinical infectious diseases : an official publication of the Infectious Diseases Society of America.

[7]  K. Bush,et al.  Kinetic interactions of tazobactam with beta-lactamases from all major structural classes , 1993, Antimicrobial Agents and Chemotherapy.

[8]  A. V. van Winkelhoff,et al.  Antibiotic susceptibilities of black-pigmented gram-negative anaerobes. , 1993, FEMS immunology and medical microbiology.

[9]  A. Scuteri,et al.  E-test: a new technique for antimicrobial susceptibility testing for periodontal microorganisms. , 1992, Journal of periodontology.

[10]  L. Gutmann,et al.  Characterization of beta-lactamases from non-Bacteroides fragilis group Bacteroides spp. belonging to seven species and their role in beta-lactam resistance , 1990, Antimicrobial Agents and Chemotherapy.

[11]  M. Jacobs,et al.  Beta-lactamase production and susceptibilities to amoxicillin, amoxicillin-clavulanate, ticarcillin, ticarcillin-clavulanate, cefoxitin, imipenem, and metronidazole of 320 non-Bacteroides fragilis Bacteroides isolates and 129 fusobacteria from 28 U.S. centers , 1990, Antimicrobial Agents and Chemotherapy.

[12]  J. Rodenburg,et al.  Occurrence of Bacteroides gingivalis, Bacteroides intermedius and Actinobacillus actinomycetemcomitans in severe periodontitis in relation to age and treatment history. , 1990, Journal of clinical periodontology.

[13]  A. Medeiros,et al.  A colorimetric procedure for measuring β-lactamase activity , 1988 .

[14]  K. Bush,et al.  Methodology for the study of beta-lactamases , 1986, Antimicrobial Agents and Chemotherapy.

[15]  J. Slots,et al.  The occurrence of Actinobacillus actinomycetemcomitans, Bacteroides gingivalis and Bacteroides intermedius in destructive periodontal disease in adults. , 1986, Journal of clinical periodontology.

[16]  K. Watanabe,et al.  The beta-lactamases of genus Bacteroides. , 1983, The Journal of antibiotics.

[17]  J. Slots,et al.  Black-pigmented Bacteroides spp. in the human oral cavity , 1981, Infection and immunity.

[18]  E. Bruck,et al.  National Committee for Clinical Laboratory Standards. , 1980, Pediatrics.

[19]  A. Harris,et al.  The use of analytical isoelectric focusing for detection and identification of beta-lactamases. , 1975, Journal of general microbiology.

[20]  A. Samuni.  A direct spectrophotometric assay and determination of Michaelis constants for the beta-lactamase reaction. , 1975, Analytical biochemistry.