Resistance of Escherichia coli to penicillins. II. An improved mapping of the ampA gene.

The first mutation step towards ampicillin resistance in Escherichia coli occurs in the ampA gene, and gives resistance to a D, L-ampicillin concentration of 10 μg/ml. Using interrupted conjugation and transduction experiments ampA was found to be located at 82 min on the time scale of Taylor & Thoman (1964). A number of adjacent markers were studied and the probable gene order of the ampA region was found to be argH-metA-uvrA-ampA-purA-fdp-pyrB . Two independent alleles of ampA were cotransduced with fdp + and purA + . The phenotypic expression of ampA in a purA strain has been investigated.

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