Inhibition of aflatoxin biosynthesis by tolnaftate

Tolnaftate [2-napthyl-N-methyl-N-(m-tolyl)thionocarbamate], an antifungal drug, is widely used to control superficial fungal infections in humans and other animals. In this study the effect of tolnaftate on aflatoxin biosynthesis by Aspergillus parasiticus NRRL 3240 was investigated. Tolnaftate changed the morphology of A. parasiticus to yeastlike forms and inhibited aflatoxin formation. The formation of aflatoxin G was blocked considerably, indicating a metabolic block in the conversion of aflatoxin B to aflatoxin G. The incorporation of [1-14C]acetate into aflatoxin was significantly inhibited at a concentration of 1 mM tolnaftate. The presence of zinc in the resuspension buffer resulted in reversal of the tolnaftate-induced inhibition of aflatoxin G1 biosynthesis.

[1]  T. A. Venkitasubramanian,et al.  Effect of zinc on adenine nucleotide pools in relation to aflatoxin biosynthesis in Aspergillus parasiticus , 1976, Applied and environmental microbiology.

[2]  T. A. Venkitasubramanian,et al.  Inhibition of aflatoxin formation by 2-mercaptoethanol , 1976, Applied and environmental microbiology.

[3]  J. Rodricks.  Mycotoxins and other fungal related food problems. , 1976 .

[4]  S. Gupta,et al.  Effect of some inhibitors on aflatoxin production in a synthetic medium and on the incorporation of acetate-1-14C into aflatoxins by resting mycelia ofAspergillus parasiticus , 1976, Bulletin of environmental contamination and toxicology.

[5]  S. Gupta,et al.  Synthesis of aflatoxins by the non-growing mycelia of Aspergillus parasiticus and the effect of inhibitors. , 1975, Journal of general microbiology.

[6]  T. A. Venkitasubramanian,et al.  Production of aflatoxin on soybeans. , 1975, Applied microbiology.

[7]  S. Gupta,et al.  Factors influencing the biosynthesis of aflatoxins. , 1975, Indian journal of biochemistry & biophysics.

[8]  G. Bean,et al.  USE OF DIMETHYL SULFOXIDE TO CONTROL AFLATOXIN PRODUCTION * , 1975, Annals of the New York Academy of Sciences.

[9]  R. J. Cole,et al.  Inhibition of aflatoxin production and tentative identification of an aflatoxin intermediate "versiconal acetate" from treatment with dichlorvos. , 1974, Applied microbiology.

[10]  R. W. Detroy,et al.  Aflatoxin and anthraquinone biosynthesis by nitrosoguanidine-derived mutants of Aspergillus parasiticus. , 1973, Canadian journal of microbiology.

[11]  D. Hsieh.  Inhibition of aflatoxin biosynthesis of dichlorvos. , 1973, Journal of agricultural and food chemistry.

[12]  T. Reddy,et al.  High aflatoxin production on a chemically defined medium. , 1971, Applied microbiology.

[13]  R. W. Detroy,et al.  Induction of yeastlike development in Aspergillus parasiticus. , 1971, Journal of general microbiology.

[14]  C. W. Hesseltine,et al.  Secondary biosynthesis of aflatoxin B in Aspergillus parasiticus. , 1970, Canadian journal of microbiology.

[15]  J. Adye,et al.  INCORPORATION OF LABELLED COMPOUNDS INTO AFLATOXINS. , 1964, Biochimica et biophysica acta.

[16]  G. Medoff,et al.  Antifungal agents: recent developments. , 1977, Annual review of microbiology.

[17]  A. Venkitasubramanian,et al.  Production ofAflatoxin onSoybeans , 1975 .

[18]  K. Maggon,et al.  Effect of Trace Metals on Aflatoxin Production by Aspergillus flavus , 1973 .

[19]  E. Weinberg.  Biosynthesis of Secondary Metabolites: Roles of Trace Metals , 1969 .

[20]  P. J. Damato.  Treatment of otomycosis due to aspergillus niger with tolnaftate , 1966 .

[21]  B. F. Nesbitt,et al.  A spectrophotometric method for determining the aflatoxins , 1965 .

[22]  A. Padhye,et al.  Antifungal Agents , 2022 .