The presence of phosphate-binding protein in inner mitochondrial membrane.

Phosphate-binding protein(s) was found in the inner mitochondrial membrane of calf heart by Sephadex G-200 and G-25 gel filtration. The binding activity was inhibited by N-ethylmaleimide and competed by a large amount of cold phosphate. The amount of phosphate bound to the fraction was 29 nmoles per mg of protein. Affinity chromatography with phosphate-bound Sepharose 4B confirmed the presence of phosphate-binding protein(s) in the active fraction of mitochondrial membrane fractionated by gel filtration.

[1]  F. Zoccarato,et al.  Phosphate transport across the mitochondrial membrane: The influence of thiol oxidation and of Mg++ on inhibition by mercurials , 1975, FEBS letters.

[2]  P. Hadváry,et al.  Specific binding of phosphate by a chloroform-soluble protein from rat-liver mitochondria. , 1973, European journal of biochemistry.

[3]  A. E. Senior,et al.  The isolation and properties of a peptide ionophore from beef heart mitochondria. , 1971, Biochemical and biophysical research communications.

[4]  P. Cuatrecasas,et al.  Agarose Derivatives for Purification of Protein by Affinity Chromatography , 1970, Nature.

[5]  A L Lehninger,et al.  Mitochondria and calcium ion transport. , 1970, The Biochemical journal.

[6]  S. Papa,et al.  Effect of uncouplers on the transport of anions in rat-liver mitochondria. , 1970, Biochimica et biophysica acta.

[7]  A. Fonyó.  Phosphate carrier of rat-liver mitochondria: its role in phosphate outflow. , 1968, Biochemical and biophysical research communications.

[8]  B. Pressman,et al.  Mechanism of action of valinomycin on mitochondria , 1964 .

[9]  M. Hansen,et al.  Studies on the mechanism of oxidative phosphorylation: VII. Preparation of a submitochondrial particle (ETPH) which is capable of fully coupled oxidative phosphorylation , 1964 .

[10]  P. Mitchell.  Coupling of Phosphorylation to Electron and Hydrogen Transfer by a Chemi-Osmotic type of Mechanism , 1961, Nature.

[11]  F. L. Crane,et al.  Studies on the electron transfer system. IV. The electron transfer particle. , 1956, Biochimica et biophysica acta.

[12]  M. Klingenberg,et al.  Demonstration of the relationship between the adenine nucleotide carrier and the structural changes of mitochondria as induced by adenosine 5'-diphosphate. , 1974, Biochemistry.

[13]  O. Hatase,et al.  Specific inhibition of phosphate transport in mitochondria by N-ethylmaleimide , 1973 .

[14]  Y. Hatefi,et al.  Studies on the mechanism of oxidative phosphorylation. III. Phosphorylating particle types from beef heart. , 1958, Biochimica et biophysica acta.