Comparison of enzyme-linked immunosorbent assay, fluorescence assay and indirect immunofluorescence assay in detection of Avian Leukosis Virus Subgroup J in DF1 cells.

The levels of immunofluorescence assay (FA), indirect immunofluorescence assay (IFA), and enzyme-linked immunosorbent assay (ELISA) in the dynamic detection of the avian leukosis virus subgroup J (ALV-J) into DF1 cells were compared and evaluated. The rNX0101 strain of ALV-J was inoculated into DF-1 cells at three different concentrations (1×10, 1×10, 1×10 TCID50). Results showed that with ELISA, the rNX0101 strain was first detected on the 3 day at a concentration of 1×10 TCID50 and on the first day at concentrations of 1×10 and 1×10 TCID50. FA failed to detect the positive cells until the 3 day after inoculation at a concentration of 1×10 TCID50. IFA detected the positive cells in the culture at all concentrations from the 1 day to the 6 day, except for the 1 day, when used at a concentration of 1×10 TCID50. The ratios of the positively infected cells highly conformed to the trend in inoculation concentration on the same days, and IFA exhibited higher sensitivity than did FA and ELISA in the dynamic detection of ALV-J.

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