A label‐free protein microfluidic array for parallel immunoassays

A label‐free protein microfluidic array for immunoassays based on the combination of imaging ellipsometry and an integrated microfluidic system is presented. Proteins can be patterned homogeneously on substrate in array format by the microfluidic system simultaneously. After preparation, the protein array can be packed in the microfluidic system which is full of buffer so that proteins are not exposed to denaturing conditions. With simple microfluidic channel junction, the protein microfluidic array can be used in serial or parallel format to analyze single or multiple samples simultaneously. Imaging ellipsometry is used for the protein array reading with a label‐free format. The biological and medical applications of the label‐free protein microfluidic array are demonstrated by screening for antibody–antigen interactions, measuring the concentration of the protein solution and detecting five markers of hepatitis B.

[1]  Peiyu Li,et al.  A novel anti-CD146 monoclonal antibody, AA98, inhibits angiogenesis and tumor growth. , 2003, Blood.

[2]  T. Kodadek.  Protein microarrays: prospects and problems. , 2001, Chemistry & biology.

[3]  R. Azzam,et al.  Ellipsometry and polarized light : North Holland, Amsterdam, 1987 (ISBN 0-444-87016-4). xvii + 539 pp. Price Dfl. 75.00. , 1987 .

[4]  S. Schreiber,et al.  Printing proteins as microarrays for high-throughput function determination. , 2000, Science.

[5]  Petra Pavlickova,et al.  Application of self‐assembly techniques in the design of biocompatible protein microarray surfaces , 2002, Electrophoresis.

[6]  G M Whitesides,et al.  Fabrication of topologically complex three-dimensional microfluidic systems in PDMS by rapid prototyping. , 2000, Analytical chemistry.

[7]  G. Whitesides,et al.  Patterned deposition of cells and proteins onto surfaces by using three-dimensional microfluidic systems. , 2000, Proceedings of the National Academy of Sciences of the United States of America.

[8]  R. Azzam,et al.  Ellipsometry and polarized light , 1977 .

[9]  D. J. Harrison,et al.  Label-free reading of microarray-based immunoassays with surface plasmon resonance imaging. , 2004, Analytical chemistry.

[10]  C. Urbaniczky,et al.  Integrated fluid handling system for biomolecular interaction analysis. , 1991, Analytical chemistry.

[11]  R. Seethala,et al.  A homogeneous, fluorescence polarization assay for src-family tyrosine kinases. , 1997, Analytical biochemistry.

[12]  Hans Arwin,et al.  Spectroscopic ellipsometry and biology: recent developments and challenges , 1998 .

[13]  Gary Hardiman,et al.  Protein microarrays: challenges and promises. , 2002, Pharmacogenomics.

[14]  M. Doyle,et al.  Kinetic analysis of a protein antigen-antibody interaction limited by mass transport on an optical biosensor. , 1997, Biophysical chemistry.

[15]  J. Malherbe,et al.  Thin Solid Films , 2008 .

[16]  Hans Arwin,et al.  Imaging ellipsometry revisited: Developments for visualization of thin transparent layers on silicon substrates , 1996 .

[17]  K. Loiacono,et al.  A fluorescence polarization based Src-SH2 binding assay. , 1997, Analytical biochemistry.

[18]  T. Jovin,et al.  Rapid characterization of green fluorescent protein fusion proteins on the molecular and cellular level by fluorescence correlation microscopy. , 1999, Proceedings of the National Academy of Sciences of the United States of America.

[19]  Hong-Gang Zhang,et al.  Evaluation of a new CA15-3 protein assay method: optical protein-chip system for clinical application. , 2005, Clinical chemistry.

[20]  Patrick Hunziker,et al.  Modeling and Optimization of High-Sensitivity, Low-Volume Microfluidic-Based Surface Immunoassays , 2005, Biomedical microdevices.

[21]  Hans Arwin,et al.  Is ellipsometry suitable for sensor applications , 2001 .

[22]  D. Ullmann,et al.  S' subsite mapping of serine proteases based on fluorescence resonance energy transfer. , 1999, Biochimica et biophysica acta.

[23]  I Lundström,et al.  A biosensor concept based on imaging ellipsometry for visualization of biomolecular interactions. , 1995, Analytical biochemistry.

[24]  David Juncker,et al.  Simultaneous detection of C-reactive protein and other cardiac markers in human plasma using micromosaic immunoassays and self-regulating microfluidic networks. , 2004, Biosensors & bioelectronics.

[25]  David Juncker,et al.  High-sensitivity miniaturized immunoassays for tumor necrosis factor alpha using microfluidic systems. , 2004, Lab on a chip.

[26]  Ute Drechsler,et al.  Autonomous microfluidic capillary system. , 2002, Analytical chemistry.