In vivo micro-CT imaging of rat brain glioma: A comparison with 3T MRI and histology

The aim of this study was to evaluate the potential of a novel micro-CT system to image in vivo the extent of tumor in a rat model of malignant glioma compared to 3T magnetic resonance imaging (MRI) and histology. Fourteen animals underwent double dose contrast-enhanced imaging with micro-CT and 3T MRI using a clinical machine at day 10 after stereotactic F98 glioma cell implantation. Calculation of the volume of the contrast-uptaking part of the tumor was done by manually outlining the tumor contours by two experienced neuroradiologists. The micro-CT- and MRI-derived tumor volumes were compared to histology as gold standard (hematoxylin and eosin staining and fluorescence staining). There was high interobserver reability regarding the tumor volumes (Crombach's alpha>0.81). Also, there was good correlation of micro-CT- and high-field MRI-derived tumor volumes compared to histology: 72+/-21 mm3 and 69+/-23 mm3 compared to 81+/-14 mm3, respectively (r>0.76). Both the micro-CT- and MRI-derived tumor volumes were not significantly smaller compared to histology (P>0.14). In conclusion, micro-CT allows in vivo imaging of the contrast-enhancing part of experimental gliomas with an accuracy comparable to high-field MRI.

[1]  L. de Ridder,et al.  In vitro and in vivo models for the study of brain tumour invasion. , 1997, Anticancer research.

[2]  S. Ulmer,et al.  Analysis of mouse brain using a clinical 1.5 T scanner and a standard small loop surface coil , 2006, Brain Research.

[3]  G Allan Johnson,et al.  4-D Micro-CT of the Mouse Heart , 2005, Molecular imaging.

[4]  W. Block,et al.  Molekulare Bildgebung von Apoptose und Nekrose : Zellbiologische Grundlagen und Einsatz in der Onkologie , 2006 .

[5]  E. Ritman.  Micro-computed tomography-current status and developments. , 2004, Annual review of biomedical engineering.

[6]  C. Giller,et al.  Detection of experimentally induced brain tumors in rats using high resolution computed tomography , 2005, Neurological research.

[7]  B. Ross,et al.  Magnetic Resonance Imaging of Ethyl-nitrosourea-induced Rat Gliomas: A Model for Experimental Therapeutics of Low-grade Gliomas , 2001, Journal of Neuro-Oncology.

[8]  D. Louis,et al.  Molecular pathways in the formation of gliomas , 1995, Glia.

[9]  G. Reifenberger,et al.  Expression of vimentin and glial fibrillary acidic protein in ethylnitrosourea-induced rat gliomas and glioma cell lines , 2004, Acta Neuropathologica.

[10]  J. Tonn,et al.  Spontaneous regression of experimental gliomas—An immunohistochemical and MRI study of the C6 glioma spheroid implantation model , 2004, Experimental Neurology.

[11]  Heidi Phillips,et al.  3D micro-CT imaging of the postmortem brain , 2008, Journal of Neuroscience Methods.

[12]  J. Boone,et al.  Small-animal X-ray dose from micro-CT. , 2004, Molecular imaging.

[13]  T. Chenevert,et al.  Growth kinetics and treatment response of the intracerebral rat 9L brain tumor model: a quantitative in vivo study using magnetic resonance imaging. , 1995, Clinical cancer research : an official journal of the American Association for Cancer Research.

[14]  M. Fukui,et al.  Apparent diffusion coefficient (ADC) and magnetization transfer contrast (MTC) mapping of experimental brain tumor. , 1997, Acta neurochirurgica. Supplement.

[15]  S. Obenauer,et al.  Eigenschaften und Anwendungen der Flächendetektor-basierten Volumen-Computertomographie , 2006 .

[16]  B. Ross,et al.  31P NMR spectroscopy of the in vivo metabolism of an intracerebral glioma in the rat , 1988, Magnetic resonance in medicine.

[17]  C. Nimsky,et al.  Small interfering RNA–mediated xCT silencing in gliomas inhibits neurodegeneration and alleviates brain edema , 2008, Nature Medicine.

[18]  Imaging of single human carcinoma cells in vitro using a clinical whole‐body magnetic resonance scanner at 3.0 T , 2005, Magnetic resonance in medicine.

[19]  M. Berens,et al.  "...those left behind." Biology and oncology of invasive glioma cells. , 1999, Neoplasia.

[20]  W Semmler,et al.  Initial Experience with a Novel Low-Dose Micro-CT System , 2007, RoFo : Fortschritte auf dem Gebiete der Rontgenstrahlen und der Nuklearmedizin.

[21]  E. Ritman,et al.  Corporeal structural and vascular micro architecture with X-ray micro computerized tomography in normal and diabetic rabbits: histopathological correlation. , 2001, The Journal of urology.