Simultaneous Rates of Ribonucleic Acid and Deoxyribonucleic Acid Syntheses for Estimating Growth and Cell Division of Aquatic Microbial Communities

A method for measuring rates of ribonucleic acid (RNA) and deoxyribonucleic acid (DNA) syntheses using a single radioactive precursor has been devised and tested using bacterial cultures and natural assemblages of marine and freshwater microorganisms. The procedure is based upon the uptake and incorporation of exogenous [3H]adenine into cellular adenosine triphosphate and deoxyadenosine triphosphate pools which serve as the immediate precursors for the adenine incorporated into RNA and DNA, respectively. It is proposed that the DNA/RNA rate ratio is correlated with the specific growth rate of microorganisms and can be used as an index for estimating and comparing the productivities of microbial assemblages in nature. This technique can also be used to detect discontinuous growth and cell division processes which frequently occur in surface plankton populations. The DNA/RNA rate ratios measured in a variety of aquatic ecosystems ranged from 3.3 to 31.8% without significant correlation to total microbial biomass.

[1]  H. Munro.  The determination of nucleic acids. , 2006, Methods of biochemical analysis.

[2]  J. Fuhrman,et al.  Bacterioplankton Secondary Production Estimates for Coastal Waters of British Columbia, Antarctica, and California , 1980, Applied and environmental microbiology.

[3]  D. Karl.  Measurement of Microbial Activity and Growth in the Ocean by Rates of Stable Ribonucleic Acid Synthesis , 1979, Applied and environmental microbiology.

[4]  D. Karl,et al.  Methodology and measurement of adenylate energy charge ratios in environmental samples , 1978 .

[5]  R. Benigni,et al.  Macromolecule Synthesis of Escherichia coli BB at a Lower or Transient Growth State , 1978, Applied and environmental microbiology.

[6]  H. Bremer,et al.  Establishment of exponential growth after a nutritional shift-up in Escherichia coli B/r: accumulation of deoxyribonucleic acid, ribonucleic acid, and protein , 1977, Journal of bacteriology.

[7]  S. Kuno,et al.  Macromolecule synthesis in Escherichia coli BB under various growth conditions , 1976, Applied and environmental microbiology.

[8]  P. Dennis,et al.  Macromolecular Composition During Steady-State Growth of Escherichia coli B/r , 1974, Journal of bacteriology.

[9]  H. E. Kubitschek.  Constancy of the ratio of DNA to cell volume in steady-state cultures of Escherichia coli B-r. , 1974, Biophysical journal.

[10]  N. Pace,et al.  Structure and synthesis of the ribosomal ribonucleic acid of prokaryotes. , 1973, Bacteriological reviews.

[11]  P. Dennis.  Regulation of ribosomal and transfer ribonucleic acid synthesis in Escherichia coli B-r. , 1972, The Journal of biological chemistry.

[12]  H. Rosenkranz,et al.  Nalidixic Acid and the Metabolism of Escherichia coli , 1970, Journal of bacteriology.

[13]  V. Leick.  Ratios between Contents of DNA, RNA and Protein in Different Micro-organisms as a Function of Maximal Growth Rate , 1968, Nature.

[14]  A. Larsson,et al.  Significance of ribonucleotide reduction in the biosynthesis of deoxyribonucleotides in Escherichia coli. , 1967, European journal of biochemistry.

[15]  T. D. Brock.  Bacterial Growth Rate in the Sea: Direct Analysis by Thymidine Autoradiography , 1967, Science.

[16]  K. Cantor.  Control of Macromolecular Synthesis , 1966, The Yale Journal of Biology and Medicine.

[17]  L. R. Hill.  An index to deoxyribonucleic acid base compositions of bacterial species. , 1966, Journal of general microbiology.

[18]  R Rosset,et al.  Ribonucleic acid composition of bacteria as a function of growth rate. , 1966, Journal of molecular biology.

[19]  O. Maaløe,et al.  Dependency on medium and temperature of cell size and chemical composition during balanced grown of Salmonella typhimurium. , 1958, Journal of general microbiology.

[20]  H. Wade,et al.  The nature of the fluctuating ribonucleic acid in Escherichia coli. , 1957, The Biochemical journal.

[21]  P. Reichard,et al.  Reduction of ribonucleotides. , 1979, Annual review of biochemistry.

[22]  R. S. Tobin,et al.  Tritiated thymidine incorporation as a measure of microbial activity in lake sediments , 1978 .

[23]  D. Nierlich.  Regulation of bacterial growth, RNA, and protein synthesis. , 1978, Annual review of microbiology.

[24]  O. Maaløe.  An Analysis of Bacterial Growth , 1970 .

[25]  Niels Ole Kjeldgaabd.  Regulation of Nucleic Acid and Protein Formation in Bacteria , 1967 .

[26]  W. C. Hutchison,et al.  The determination of nucleic acids in biological materials. A review. , 1961, The Analyst.