Association of insulin receptor substrate-1 with integrins.

Insulin stimulation was found to promote association of the alpha v beta 3 integrin (a vitronectin receptor) with insulin receptor substrate-1 (IRS-1), an intracellular protein that mediates insulin signaling by binding other signaling molecules, including growth factor receptor-bound protein 2 (Grb2) and phosphatidylinositol-3' kinase. Insulin-treated cells expressing the alpha v beta 3 integrin showed 2.5 times more DNA synthesis when plated on vitronectin than on other substrates, whereas cells expressing another vitronectin receptor, alpha v beta 5, did not show this difference. The association between integrin and IRS-1 may be a mechanism for the synergistic action of growth factor and extracellular matrix receptors.

[1]  John Calvin Reed,et al.  Anchorage dependence, integrins, and apoptosis , 1994, Cell.

[2]  G. Lienhard,et al.  Insulin signalling: the role of insulin receptor substrate 1. , 1994, Trends in cell biology.

[3]  S. Frisch,et al.  Disruption of epithelial cell-matrix interactions induces apoptosis , 1994, The Journal of cell biology.

[4]  J. Olefsky,et al.  Insulin receptor substrate 1 is required for insulin-mediated mitogenic signal transduction. , 1994, Proceedings of the National Academy of Sciences of the United States of America.

[5]  C. Kahn,et al.  The insulin signaling system. , 1994, The Journal of biological chemistry.

[6]  M. Schwartz,et al.  The extracellular matrix as a cell survival factor. , 1993, Molecular biology of the cell.

[7]  E. Pasquale,et al.  The alpha v beta 3 integrin associates with a 190-kDa protein that is phosphorylated on tyrosine in response to platelet-derived growth factor. , 1993, The Journal of biological chemistry.

[8]  J. Parsons,et al.  Focal adhesion kinase: an integrin-linked protein tyrosine kinase. , 1993, Trends in cell biology.

[9]  S. Haskill,et al.  Signal transduction from the extracellular matrix , 1993, The Journal of cell biology.

[10]  D. Leavesley,et al.  Requirement of the integrin beta 3 subunit for carcinoma cell spreading or migration on vitronectin and fibrinogen , 1992, The Journal of cell biology.

[11]  Richard O. Hynes,et al.  Integrins: Versatility, modulation, and signaling in cell adhesion , 1992, Cell.

[12]  D. Schubert,et al.  Substratum-growth factor collaborations are required for the mitogenic activities of activin and FGF on embryonal carcinoma cells , 1991, The Journal of cell biology.

[13]  E. Wayner,et al.  Integrins alpha v beta 3 and alpha v beta 5 contribute to cell attachment to vitronectin but differentially distribute on the cell surface , 1991, The Journal of cell biology.

[14]  M. Raff,et al.  Extracellular matrix-associated molecules collaborate with ciliary neurotrophic factor to induce type-2 astrocyte development , 1990, The Journal of cell biology.

[15]  Jeffrey W. Smith,et al.  A novel vitronectin receptor integrin (α v β x ) is responsible for distinct adhesive properties of carcinoma cells , 1989, Cell.

[16]  E. Dejana,et al.  Fibronectin and vitronectin regulate the organization of their respective Arg-Gly-Asp adhesion receptors in cultured human endothelial cells , 1988, The Journal of cell biology.

[17]  E. Kohn,et al.  Insulin-like growth factors stimulate chemotaxis in human melanoma cells. , 1988, Biochemical and biophysical research communications.

[18]  T. Dull,et al.  A mutant insulin receptor with defective tyrosine kinase displays no biologic activity and does not undergo endocytosis. , 1987, The Journal of biological chemistry.

[19]  E Ruoslahti,et al.  New perspectives in cell adhesion: RGD and integrins. , 1987, Science.

[20]  E. Ruoslahti,et al.  [27] Arginine-glycine-aspartic acid adhesion receptors , 1987 .

[21]  M. Kasuga,et al.  Insulin-like growth factors, insulin, and epidermal growth factor cause rapid cytoskeletal reorganization in KB cells. Clarification of the roles of type I insulin-like growth factor receptors and insulin receptors. , 1986, The Journal of biological chemistry.

[22]  C. Stiles,et al.  Regulation of cytoskeletal architecture by platelet-derived growth factor, insulin and epidermal growth factor. , 1984, Experimental cell research.

[23]  B. Geiger,et al.  Epidermal growth factor induces redistribution of actin and alpha-actinin in human epidermal carcinoma cells. , 1981, Experimental cell research.

[24]  C. O'neill,et al.  Anchorage and growth regulation in normal and virus‐transformed cells , 1968, International journal of cancer.