Use of bacterial cellulose film for repair of bile duct injury in pigs

Background/objective The aim was to evaluate the use of bacterial cellulose film and bile duct autograft in repairing critical common bile duct injury in pigs. Methods A prospective experimental analytical study was carried out on 20 Sus Domesticus, Piau suidae swine, divided into a control group (n = 10) and an experimental group (n = 10) divided into two subgroups: bacterial cellulose film E1 and bacterial cellulose film E2 to which bacterial cellulose film was randomly allocated. The control group underwent two complete critical common bile duct sections 10 mm apart, while the experimental group with a single critical common bile duct defect underwent a 10 mm section of the longitudinal shaft with edge resection. The defects in the control group were treated with end-to-end conventional anastomosis using polyglycolic 6–0 surgical thread and the experimental group with bacterial cellulose film by continuous suture using the same material. The animals were clinically evaluated throughout the experiment on days D150 (bacterial cellulose film E1), D225 (control group), and D330 (bacterial cellulose film E2) and by intraoperative ultrasound examination related to histopathological and biochemical findings. Results The intraoperative ultrasonography detected the changes resulting from the common bile duct anastomosis in the control group that produced a considerable incidence of ductal narrowing and obstruction to the biliary flow. In the bacterial cellulose film E2 group, there was an increase in inflammation intensity, granulomatous reaction, fibrosis, and vessels density, without producing bile duct dilation in the ultrasonography assessment. Biochemical analysis of liver enzymes yielded results in the normal range confirming preservation of liver function at the different post-surgery time points. Conclusion Bacterial cellulose film, when used as a graft for bile duct repair, proved to be a biocompatible material that produced a complete healing process and biliary flow continuity.

[1]  S. Palmucci,et al.  Iatrogenic bile duct injury: impact and management challenges , 2019, Clinical and experimental gastroenterology.

[2]  J. Joh,et al.  Intraoperative Ultrasonography as a Guidance for Dividing Bile Duct During Laparoscopic Living Donor Hepatectomy , 2019, Annals of transplantation.

[3]  A. Chong,et al.  Benign biliary strictures: prevalence, impact, and management strategies , 2019, Clinical and experimental gastroenterology.

[4]  Raquel Nogueira Cordeiro,et al.  Resection of a giant nonparasitic splenic cyst by minilaparoscopy , 2019, Journal of minimal access surgery.

[5]  J. Aguiar,et al.  Experimental study of femoral vein reconstruction with sugarcane biopolymer tubular graft. , 2018, Revista do Colegio Brasileiro de Cirurgioes.

[6]  S. Glaser,et al.  Recent advances in understanding bile duct remodeling and fibrosis , 2018, F1000Research.

[7]  A. Chandra,et al.  Intraoperative ultrasonography of the biliary tract using saline as a contrast agent: a fast and accurate technique to identify complex biliary anatomy , 2017, Canadian journal of surgery. Journal canadien de chirurgie.

[8]  Jiahong Dong,et al.  Surgical management for bile duct injury. , 2017, Bioscience trends.

[9]  M. Nooghabi,et al.  Iatrogenic injuries of the extrahepatic biliary system. , 2017, The Journal of surgical research.

[10]  F. Bösch,et al.  Bile Duct Injury after Cholecystectomy: Surgical Therapy , 2017, Visceral Medicine.

[11]  G. Torzilli,et al.  State of the Art of Intraoperative Ultrasound in Liver Surgery: Current Use for Resection-guidance. , 2017, Chirurgia.

[12]  B. Jiang,et al.  Modified biliary-enteric anastomosis for congenital choledochal cyst: clinical and prognostic analysis of 91 cases , 2017, Pediatric Surgery International.

[13]  Frederico de Melo Tavares de Lima,et al.  Biocompatible bacterial cellulose membrane in dural defect repair of rat , 2017, Journal of Materials Science: Materials in Medicine.

[14]  S. Zangan,et al.  Benign Biliary Strictures , 2016, Seminars in Interventional Radiology.

[15]  F. C. M. Pinto,et al.  Bioprosthetic mesh of bacterial cellulose for treatment of abdominal muscle aponeurotic defect in rat model , 2016, Journal of Materials Science: Materials in Medicine.

[16]  G. Mclachlan,et al.  Meta-analysis of the diagnostic accuracy of laparoscopic ultrasonography and intraoperative cholangiography in detection of common bile duct stones , 2016 .

[17]  F. Paumgartten,et al.  Acute toxicity, cytotoxicity, genotoxicity and antigenotoxic effects of a cellulosic exopolysaccharide obtained from sugarcane molasses. , 2016, Carbohydrate polymers.

[18]  M.C. Leal,et al.  Treatment of tympanic membrane perforation using bacterial cellulose: a randomized controlled trial , 2015, Brazilian journal of otorhinolaryngology.

[19]  B. Vasconcelos,et al.  The biopolymer sugarcane as filling material of critical defects in rats. , 2016, Acta cirurgica brasileira.

[20]  G. Carvalho,et al.  Type IV Mirizzi Syndrome Treated with Hepaticoduodenostomy and Minilaparoscopy , 2016 .

[21]  M. Mercado,et al.  Iatrogenic bile duct injury with loss of confluence. , 2015, World journal of gastrointestinal surgery.

[22]  F. Sampaio,et al.  The Biocompatibility of a Cellulose Exopolysaccharide Implant in the Rabbit Bladder When Compared With Dextranomer Microspheres Plus Hyaluronic Acid. , 2015, Urology.

[23]  Yitao Ding,et al.  Repair of extrahepatic bile duct defect using a collagen patch in a Swine model. , 2015, Artificial organs.

[24]  F. C. M. Pinto,et al.  Biocompatibility and cutaneous reactivity of cellulosic polysaccharide film in induced skin wounds in rats , 2015, Journal of Materials Science: Materials in Medicine.

[25]  A. Gelrud,et al.  Biliary strictures: diagnostic considerations and approach , 2014, Gastroenterology report.

[26]  A. Montgomery,et al.  Bile Duct Injuries Associated With 55,134 Cholecystectomies: Treatment and Outcome from a National Perspective , 2015, World Journal of Surgery.

[27]  José Lamartine de Andrade Aguiar,et al.  Spongy film of cellulosic polysaccharide as a dressing for aphthous stomatitis treatment in rabbits. , 2014, Acta cirurgica brasileira.

[28]  F. O. Vilar,et al.  Biopolymer Sponge for High Grade Renal Trauma: An Experimental Study in Rabbits , 2014 .

[29]  P. T. Poyatos,et al.  Reconstrucción del conducto biliar mediante tubos tridimensionales de colágeno , 2013 .

[30]  E. Brătucu,et al.  The role of intraoperative ultrasound in establishing the surgical strategy regarding hepato-bilio-pancreatic pathology. , 2013, Chirurgia.

[31]  Alex Augusto Silva,et al.  Estudo ultrassonográfico morfométrico do fígado e trato biliar de suínos submetidos a obstrução biliar experimental , 2013 .

[32]  A. P. Pérez Alonso,et al.  [Bile duct reconstruction using 3-dimensional collagen tubes]. , 2013, Cirugia espanola.

[33]  M.C. Leal,et al.  Sugarcane biopolymer membrane: experimental evaluation in the middle ear , 2015, Brazilian journal of otorhinolaryngology.

[34]  Y. Ikada,et al.  An Extrahepatic Bile Duct Grafting Using a Bioabsorbable Polymer Tube , 2012, Journal of Gastrointestinal Surgery.

[35]  W. Melvin,et al.  Novel reconstruction of the extrahepatic biliary tree with a biosynthetic absorbable graft. , 2011, HPB.

[36]  J. Aguiar,et al.  A new vascular substitute: femoral artery angioplasty in dogs using sugarcane biopolymer membrane patch - hemodynamic and histopathologic evaluation , 2007 .

[37]  F. Mortensen,et al.  Reconstruction of the common bile duct by a vascular prosthetic graft: an experimental study in pigs. , 2005, Journal of hepato-biliary-pancreatic surgery.