Solution NMR of membrane proteins: practice and challenges
暂无分享,去创建一个
[1] R. Hodges,et al. Effect of trifluoroethanol on protein secondary structure: an NMR and CD study using a synthetic actin peptide. , 1992, Biochemistry.
[2] J. Klein-Seetharaman,et al. Differential dynamics in the G protein-coupled receptor rhodopsin revealed by solution NMR. , 2004, Proceedings of the National Academy of Sciences of the United States of America.
[3] J U Bowie,et al. Building a Thermostable Membrane Protein* , 2000, The Journal of Biological Chemistry.
[4] A. McGuire,et al. Internal and overall motions of the translation factor eIF4E: Cap binding and insertion in a CHAPS detergent micelle , 1998, Journal of biomolecular NMR.
[5] A. Bax,et al. A simple apparatus for generating stretched polyacrylamide gels, yielding uniform alignment of proteins and detergent micelles* , 2001, Journal of biomolecular NMR.
[6] C. Sanders,et al. Topology and secondary structure of the N-terminal domain of diacylglycerol kinase. , 2002, Biochemistry.
[7] L. Lian,et al. Labelling approaches for protein structural studies by solution-state and solid-state NMR , 2001 .
[8] V. V. Kumar,et al. Lanthanide-induced phosphorus-31 NMR downfield chemical shifts of lysophosphatidylcholines are sensitive to lysophospholipid critical micelle concentration. , 1991, Biophysical journal.
[9] P. Schmieder,et al. Heteronuclear Multidimensional NMR Spectroscopy of Solubilized Membrane Proteins: Resonance Assignment of Native Bacteriorhodopsin , 2002, Chembiochem : a European journal of chemical biology.
[10] D. Mierke,et al. Peptide hormone binding to G‐protein‐coupled receptors: Structural characterization via NMR techniques , 2001, Medicinal research reviews.
[11] A. Bax,et al. Characterization of Phospholipid Mixed Micelles by Translational Diffusion , 2004, Journal of biomolecular NMR.
[12] D. W. Bolen,et al. Protein stabilization by naturally occurring osmolytes. , 2001, Methods in molecular biology.
[13] L. Kay,et al. The integral membrane enzyme PagP alternates between two dynamically distinct states. , 2004, Proceedings of the National Academy of Sciences of the United States of America.
[14] D. Otzen,et al. Using micellar mole fractions to assess membrane protein stability in mixed micelles. , 2005, Biochimica et biophysica acta.
[15] P. Domaille,et al. An Approach to the Structure Determination of Larger Proteins Using Triple Resonance NMR Experiments in Conjunction with Random Fractional Deuteration , 1996 .
[16] G. Otting,et al. Site-specific Labelling with a Metal Chelator for Protein-structure Refinement , 2004, Journal of biomolecular NMR.
[17] J H Prestegard,et al. NMR structures of biomolecules using field oriented media and residual dipolar couplings , 2000, Quarterly Reviews of Biophysics.
[18] L. Kay,et al. Nuclear magnetic resonance spectroscopy of high-molecular-weight proteins. , 2004, Annual review of biochemistry.
[19] J. Bowie,et al. A method for assessing the stability of a membrane protein. , 1997, Biochemistry.
[20] A. Gunasekera,et al. Nuclear magnetic resonance structural studies of a potassium channel-charybdotoxin complex. , 2005, Biochemistry.
[21] Backbone resonance assignment in large protonated proteins using a combination of new 3D TROSY-HN(CA)HA, 4D TROSY-HACANH and 13C-detected HACACO experiments , 2003, Journal of biomolecular NMR.
[22] Gianluigi Veglia,et al. Serine 16 phosphorylation induces an order-to-disorder transition in monomeric phospholamban. , 2005, Biochemistry.
[23] Janet L. Smith,et al. A defined protein–detergent–lipid complex for crystallization of integral membrane proteins: The cytochrome b6f complex of oxygenic photosynthesis , 2003, Proceedings of the National Academy of Sciences of the United States of America.
[24] J. Klein-Seetharaman,et al. Solution 19F nuclear Overhauser effects in structural studies of the cytoplasmic domain of mammalian rhodopsin , 2001, Proceedings of the National Academy of Sciences of the United States of America.
[25] T. Sawasaki,et al. High-throughput, genome-scale protein production method based on the wheat germ cell-free expression system , 2004, Journal of Structural and Functional Genomics.
[26] T. Ikegami,et al. Conformation of a peptide ligand bound to its G-protein coupled receptor , 2001, Nature Structural Biology.
[27] R. H. Fillingame,et al. Structural model of the transmembrane Fo rotary sector of H+-transporting ATP synthase derived by solution NMR and intersubunit cross-linking in situ. , 2002, Biochimica et biophysica acta.
[28] S. Harrison,et al. Lipid–protein interactions in double-layered two-dimensional AQP0 crystals , 2005, Nature.
[29] J. Weigelt. Single Scan, Sensitivity- and Gradient-Enhanced TROSY for Multidimensional NMR Experiments , 1998 .
[30] Wing-Yiu Choy,et al. Solution structure and dynamics of the outer membrane enzyme PagP by NMR , 2002, Proceedings of the National Academy of Sciences of the United States of America.
[31] E. Dennis,et al. Interfacial properties and critical micelle concentration of lysophospholipids. , 1989, Biochemistry.
[32] S. White,et al. Structure of a fluid dioleoylphosphatidylcholine bilayer determined by joint refinement of x-ray and neutron diffraction data. II. Distribution and packing of terminal methyl groups. , 1992, Biophysical journal.
[33] F. Separovic,et al. Solid‐state NMR Structure Determination , 2003, IUBMB life.
[34] C. Sanders,et al. Oxygen as a paramagnetic probe of membrane protein structure by cysteine mutagenesis and (19)F NMR spectroscopy. , 2002, Journal of the American Chemical Society.
[35] L. Kay,et al. Solution structure and dynamics of integral membrane proteins by NMR: a case study involving the enzyme PagP. , 2005, Methods in enzymology.
[36] Alexander Eletsky,et al. A novel strategy for the assignment of side-chain resonances in completely deuterated large proteins using 13C spectroscopy , 2003, Journal of biomolecular NMR.
[37] J. Popot,et al. NMR study of a membrane protein in detergent-free aqueous solution. , 2005, Proceedings of the National Academy of Sciences of the United States of America.
[38] T. Cross. Solid-state nuclear magnetic resonance characterization of gramicidin channel structure. , 1997, Methods in enzymology.
[39] B. Sykes,et al. Methods to study membrane protein structure in solution. , 1994, Methods in enzymology.
[40] Konstantin Pervushin,et al. Simultaneous 1H- or 2H-, 15N- and multiple-band-selective 13C-decoupling during acquisition in 13C-detected experiments with proteins and oligonucleotides , 2005, Journal of biomolecular NMR.
[41] H. Weltzien,et al. Quantitative studies on lysolecithin-mediated hemolysis. Use of ether-deoxy lysolecithin analogs with varying aliphatic chain-lengths. , 1977, Biochimica et biophysica acta.
[42] L. Kay,et al. Line narrowing in methyl-TROSY using zero-quantum 1H-13C NMR spectroscopy. , 2004, Journal of the American Chemical Society.
[43] C. Hunte,et al. Specific protein-lipid interactions in membrane proteins. , 2005, Biochemical Society transactions.
[44] J. Becker,et al. Synthetic peptides as probes for conformational preferences of domains of membrane receptors , 2005, Biopolymers.
[45] J. Klein-Seetharaman,et al. Solution NMR spectroscopy of [α-15N]lysine-labeled rhodopsin: The single peak observed in both conventional and TROSY-type HSQC spectra is ascribed to Lys-339 in the carboxyl-terminal peptide sequence , 2002, Proceedings of the National Academy of Sciences of the United States of America.
[46] M. Girvin,et al. Determination of local protein structure by spin label difference 2D NMR: the region neighboring Asp61 of subunit c of the F1F0 ATP synthase. , 1994, Biochemistry.
[47] C. Sanders,et al. Bicelles: a model membrane system for all seasons? , 1998, Structure.
[48] A. Watts. Solid-state NMR in drug design and discovery for membrane-embedded targets , 2005, Nature Reviews Drug Discovery.
[49] Masasuke Yoshida,et al. Conformational change of H+-ATPase beta monomer revealed on segmental isotope labeling NMR spectroscopy. , 2004, Journal of the American Chemical Society.
[50] S. Züger,et al. Intein-based biosynthetic incorporation of unlabeled protein tags into isotopically labeled proteins for NMR studies , 2005, Nature Biotechnology.
[51] J. Hus,et al. De novo determination of protein structure by NMR using orientational and long-range order restraints. , 2000, Journal of molecular biology.
[52] E. Gouaux,et al. Probing the folding and unfolding of wild-type and mutant forms of bacteriorhodopsin in micellar solutions: evaluation of reversible unfolding conditions. , 1999, Biochemistry.
[53] Steven O. Smith,et al. Transmembrane interactions in the activation of the Neu receptor tyrosine kinase. , 2002, Biochemistry.
[54] S. Grzesiek,et al. Solution NMR of proteins within polyacrylamide gels: Diffusional properties and residual alignment by mechanical stress or embedding of oriented purple membranes , 2000, Journal of biomolecular NMR.
[55] M. Girvin,et al. Structural changes linked to proton translocation by subunit c of the ATP synthase , 1999, Nature.
[56] J. Klein-Seetharaman,et al. NMR spectroscopy in studies of light-induced structural changes in mammalian rhodopsin: applicability of solution (19)F NMR. , 1999, Proceedings of the National Academy of Sciences of the United States of America.
[57] S. White,et al. Membrane protein folding and stability: physical principles. , 1999, Annual review of biophysics and biomolecular structure.
[58] K. Pervushin,et al. Improved TROSY-HNCA experiment with suppression of conformational exchange induced relaxation , 2001, Journal of biomolecular NMR.
[59] R. MacKinnon,et al. Semisynthesis and folding of the potassium channel KcsA. , 2002, Journal of the American Chemical Society.
[60] L. Thompson. Solid-state NMR studies of the structure and mechanisms of proteins. , 2002, Current opinion in structural biology.
[61] A. Makriyannis,et al. The conformation of the cytoplasmic helix 8 of the CB1 cannabinoid receptor using NMR and circular dichroism. , 2005, Biochimica et biophysica acta.
[62] Sow-Hsin Chen,et al. Small-angle neutron scattering techniques applied to the study of polydisperse rodlike diheptanoylphosphatidylcholine micelles , 1987 .
[63] Roland Riek,et al. NMR Structure of Mistic, a Membrane-Integrating Protein for Membrane Protein Expression , 2005, Science.
[64] Effects of anionic lipid and ion concentrations on the topology and segmental mobility of colicin Ia channel domain from solid-state NMR. , 2006, Biochemistry.
[65] D. Otzen. Folding of DsbB in mixed micelles: a kinetic analysis of the stability of a bacterial membrane protein. , 2003, Journal of molecular biology.
[66] J. Bowie,et al. How to Prepare Membrane Proteins for Solid‐State NMR: A Case Study on the α‐Helical Integral Membrane Protein Diacylglycerol Kinase from E. coli , 2005, Chembiochem : a European journal of chemical biology.
[67] Charles R Sanders,et al. Disease-related misassembly of membrane proteins. , 2004, Annual review of biophysics and biomolecular structure.
[68] W. Dowhan,et al. Molecular basis for membrane phospholipid diversity: why are there so many lipids? , 1997, Annual review of biochemistry.
[69] S. Opella,et al. NMR structure determination of a membrane protein with two transmembrane helices in micelles: MerF of the bacterial mercury detoxification system. , 2005, Biochemistry.
[70] M. Girvin,et al. An evaluation of detergents for NMR structural studies of membrane proteins , 2004, Journal of biomolecular NMR.
[71] Alexander Shekhtman,et al. Semisynthesis of a segmental isotopically labeled protein splicing precursor: NMR evidence for an unusual peptide bond at the N-extein-intein junction. , 2004, Proceedings of the National Academy of Sciences of the United States of America.
[72] D. Marsh,et al. The protein-lipid interface: perspectives from magnetic resonance and crystal structures. , 2004, Biochimica et biophysica acta.
[73] N. Go,et al. Refined structure of melittin bound to perdeuterated dodeclylphoscholine micelles as studied by 2D‐NMR and distance geometry calculation , 1991, Proteins.
[74] S. Opella,et al. Rotational diffusion of membrane proteins in aligned phospholipid bilayers by solid-state NMR spectroscopy. , 2006, Journal of magnetic resonance.
[75] G. Wagner,et al. Utilization of site-directed spin labeling and high-resolution heteronuclear nuclear magnetic resonance for global fold determination of large proteins with limited nuclear overhauser effect data. , 2000, Biochemistry.
[76] Y. Ishii,et al. Controlling residual dipolar couplings in high-resolution NMR of proteins by strain induced alignment in a gel , 2001, Journal of biomolecular NMR.
[77] G. Wider,et al. Membrane Protein–Lipid Interactions in Mixed Micelles Studied by NMR Spectroscopy with the Use of Paramagnetic Reagents , 2004, Chembiochem : a European journal of chemical biology.
[78] E. Dennis,et al. Solubilization of phospholipids by detergents. Structural and kinetic aspects. , 1983, Biochimica et biophysica acta.
[79] J. Møller,et al. Interaction of membrane proteins and lipids with solubilizing detergents. , 2000, Biochimica et biophysica acta.
[80] K. Wüthrich,et al. Physicochemical studies of the protein-lipid interactions in melittin-containing micelles. , 1979, Biochimica et biophysica acta.
[81] T. Cierpicki,et al. Charged gels as orienting media for measurement of residual dipolar couplings in soluble and integral membrane proteins. , 2004, Journal of the American Chemical Society.
[82] Volker Dötsch,et al. Efficient strategy for the rapid backbone assignment of membrane proteins. , 2005, Journal of the American Chemical Society.
[83] D. Nietlispach. Suppression of anti-TROSY lines in a sensitivity enhanced gradient selection TROSY scheme , 2005, Journal of biomolecular NMR.
[84] Wing-Yiu Choy,et al. Solution NMR-derived global fold of a monomeric 82-kDa enzyme. , 2005, Proceedings of the National Academy of Sciences of the United States of America.
[85] 1H-15N backbone resonance assignments of bacteriorhodopsin. , 1996, Pharmaceutica acta Helvetiae.
[86] L. Kay,et al. Structure and dynamics of bacteriophage IKe major coat protein in MPG micelles by solution NMR. , 1996, Biochemistry.
[87] C. Sanders,et al. Escherichia coli diacylglycerol kinase: a case study in the application of solution NMR methods to an integral membrane protein. , 1997, Biophysical journal.
[88] G. Privé,et al. Lipopeptide detergents designed for the structural study of membrane proteins , 2003, Nature Biotechnology.
[89] C. Schwarz,et al. NMR investigation of the multidrug transporter EmrE, an integral membrane protein. , 1998, European journal of biochemistry.
[90] A. Bax,et al. Micelle-induced curvature in a water-insoluble HIV-1 Env peptide revealed by NMR dipolar coupling measurement in stretched polyacrylamide gel. , 2002, Journal of the American Chemical Society.
[91] Matthias Müller,et al. Development of a Minimal Cell‐Free Translation System for the Synthesis of Presecretory and Integral Membrane Proteins , 2008, Biotechnology progress.
[92] Alan S. Stern,et al. Optimization of 13C direct detection NMR methods , 2004, Journal of biomolecular NMR.
[93] A. Bax. Weak alignment offers new NMR opportunities to study protein structure and dynamics , 2003, Protein science : a publication of the Protein Society.
[94] A. Bax,et al. Measurement of Three-bond, 13C′-13Cβ J Couplings in Human Ubiquitin by a Triple Resonance, E. COSY-type NMR Technique , 1998, Journal of biomolecular NMR.
[95] C. Sanders,et al. Customizing model membranes and samples for NMR spectroscopic studies of complex membrane proteins. , 2000, Biochimica et biophysica acta.
[96] C. Cass,et al. Glucose and nucleoside transporters of human erythrocytes: effects of detergents on immunoadsorption of a membrane protein to its monoclonal antibody. , 1991, Biochimica et biophysica acta.
[97] Lukas K. Tamm,et al. Structure of outer membrane protein A transmembrane domain by NMR spectroscopy , 2001, Nature Structural Biology.
[98] Mapping the oligomeric interface of diacylglycerol kinase by engineered thiol cross-linking: homologous sites in the transmembrane domain. , 2000, Biochemistry.
[99] T. Kigawa,et al. Expression of G protein coupled receptors in a cell-free translational system using detergents and thioredoxin-fusion vectors. , 2005, Protein expression and purification.
[100] Yan Xu,et al. Structure and dynamics of the second and third transmembrane domains of human glycine receptor. , 2005, Biochemistry.
[101] J. Rosenbusch. The critical role of detergents in the crystallization of membrane proteins. , 1990, Journal of structural biology.
[102] C. Richter,et al. Carbon‐Detected NMR Experiments To Investigate Structure and Dynamics of Biological Macromolecules , 2001, Chembiochem : a European journal of chemical biology.
[103] S. Grzesiek,et al. Charged acrylamide copolymer gels as media for weak alignment , 2002, Journal of biomolecular NMR.
[104] Charles R Sanders,et al. French Swimwear for Membrane Proteins , 2004, Chembiochem : a European journal of chemical biology.
[105] C. Carlin,et al. A Structural Model for the Membrane-bound Form of the Juxtamembrane Domain of the Epidermal Growth Factor Receptor* , 2005, Journal of Biological Chemistry.
[106] C. Yu,et al. Amyloid-like Fibril Formation in an All β-Barrel Protein Involves the Formation of Partially Structured Intermediate(s)* , 2002, The Journal of Biological Chemistry.
[107] C. Sanders,et al. On choosing a detergent for solution NMR studies of membrane proteins , 1998, Journal of biomolecular NMR.
[108] K. Wüthrich,et al. High resolution nuclear magnetic resonance studies of the conformation and orientation of melittin bound to a lipid-water interface. , 1982, Biophysical journal.
[109] L. Kay,et al. Stereospecific NMR assignments of prochiral methyls, rotameric states and dynamics of valine residues in malate synthase G. , 2004, Journal of the American Chemical Society.
[110] James H. Prestegard,et al. A Transmembrane Helix Dimer: Structure and Implications , 1997, Science.
[111] A. Bax,et al. Measurement of three-bond nitrogen-carbon J couplings in proteins uniformly enriched in nitrogen-15 and carbon-13 , 1993 .
[112] M. Baldus,et al. High-resolution solid-state NMR applied to polypeptides and membrane proteins. , 2003, Accounts of chemical research.
[113] D. Engelman,et al. Detergents modulate dimerization, but not helicity, of the glycophorin A transmembrane domain. , 1999, Journal of molecular biology.
[114] Y. Kyōgoku,et al. NMR observation of selected segments in a larger protein: central-segment isotope labeling through intein-mediated ligation. , 1999, Biochemistry.
[115] J. Bushweller,et al. Structure, dynamics and function of the outer membrane protein A (OmpA) and influenza hemagglutinin fusion domain in detergent micelles by solution NMR , 2003, FEBS letters.
[116] K Wüthrich,et al. Transverse relaxation-optimized NMR spectroscopy with the outer membrane protein OmpX in dihexanoyl phosphatidylcholine micelles , 2001, Proceedings of the National Academy of Sciences of the United States of America.
[117] L. Kay,et al. The use of 2H, 13C, 15N multidimensional NMR to study the structure and dynamics of proteins. , 1998, Annual review of biophysics and biomolecular structure.
[118] N. Nielsen,et al. Techniques and applications of NMR to membrane proteins (Review) , 2004 .
[119] Tim J Stevens,et al. Membrane proteins: the 'Wild West' of structural biology. , 2003, Trends in biochemical sciences.
[120] Laurent Verdier,et al. Novel Techniques for Weak Alignment of Proteins in Solution Using Chemical Tags Coordinating Lanthanide Ions , 2004, Journal of biomolecular NMR.
[121] G. Wider,et al. NMR structure of the integral membrane protein OmpX. , 2004, Journal of molecular biology.
[122] J. P. Walsh,et al. sn-1,2-Diacylglycerol kinase of Escherichia coli. Mixed micellar analysis of the phospholipid cofactor requirement and divalent cation dependence. , 1986, The Journal of biological chemistry.
[123] C. Aisenbrey,et al. The alignment, structure and dynamics of membrane-associated polypeptides by solid-state NMR spectroscopy. , 2004, Biochimica et biophysica acta.
[124] R. Riek,et al. Attenuated T2 relaxation by mutual cancellation of dipole-dipole coupling and chemical shift anisotropy indicates an avenue to NMR structures of very large biological macromolecules in solution. , 1997, Proceedings of the National Academy of Sciences of the United States of America.
[125] David S. Cafiso,et al. Identifying conformational changes with site-directed spin labeling , 2000, Nature Structural Biology.
[126] S. Krishnaswamy,et al. Overexpression, refolding, and purification of the major immunodominant outer membrane porin OmpC from Salmonella typhi: characterization of refolded OmpC. , 2005, Protein expression and purification.
[127] Kurt Wüthrich,et al. Lipid–protein interactions in DHPC micelles containing the integral membrane protein OmpX investigated by NMR spectroscopy , 2002, Proceedings of the National Academy of Sciences of the United States of America.
[128] Kurt Wüthrich,et al. NMR solution structure determination of membrane proteins reconstituted in detergent micelles , 2003, FEBS letters.
[129] S. Opella,et al. Experimental nuclear magnetic resonance studies of membrane proteins. , 1994, Methods in enzymology.
[130] A. Wand,et al. Preparation, characterization, and NMR spectroscopy of encapsulated proteins dissolved in low viscosity fluids , 2003, Journal of biomolecular NMR.
[131] Shigeyuki Yokoyama,et al. Conformation of ligands bound to the muscarinic acetylcholine receptor. , 2002, Molecular pharmacology.
[132] Sow-Hsin Chen,et al. The use of small-angle neutron scattering to determine the structure and interaction of dihexanoylphosphatidylcholine micelles , 1986 .
[133] V. Gaponenko,et al. Breaking symmetry in the structure determination of (large) symmetric protein dimers , 2002, Journal of biomolecular NMR.
[134] P. Yeagle,et al. Structural studies on rhodopsin. , 2002, Biochimica et biophysica acta.
[135] C. Sanders,et al. NMR assignments for a helical 40 kDa membrane protein. , 2004, Journal of the American Chemical Society.
[136] A Nevzorov,et al. Structure determination of membrane proteins by NMR spectroscopy. , 2002, Biochemistry and cell biology = Biochimie et biologie cellulaire.
[137] C. Sanders,et al. An approximate model and empirical energy function for solute interactions with a water-phosphatidylcholine interface. , 1993, Biophysical journal.
[138] D. Nebert,et al. Sulfobetaine derivatives of bile acids: nondenaturing surfactants for membrane biochemistry. , 1983, Analytical biochemistry.
[139] K. Pervushin. The use of TROSY for detection and suppression of conformational exchange NMR line broadening in biological macromolecules , 2001, Journal of biomolecular NMR.
[140] T. Torizawa,et al. Efficient production of isotopically labeled proteins by cell-free synthesis: A practical protocol , 2004, Journal of biomolecular NMR.
[141] Ad Bax,et al. Evaluation of backbone proton positions and dynamics in a small protein by liquid crystal NMR spectroscopy. , 2003, Journal of the American Chemical Society.
[142] A. Bax,et al. Determination of φ and χ1 Angles in Proteins from 13C−13C Three-Bond J Couplings Measured by Three-Dimensional Heteronuclear NMR. How Planar Is the Peptide Bond? , 1997 .
[143] A. Wand,et al. New reverse micelle surfactant systems optimized for high-resolution NMR spectroscopy of encapsulated proteins. , 2005, Langmuir : the ACS journal of surfaces and colloids.
[144] L. Kay,et al. Structural characterization of proteins with an attached ATCUN motif by paramagnetic relaxation enhancement NMR spectroscopy. , 2001, Journal of the American Chemical Society.
[145] Lan Guan,et al. An approach to membrane protein structure without crystals , 2002, Proceedings of the National Academy of Sciences of the United States of America.
[146] M. Kainosho,et al. Localisation of methionine residues in bacteriorhodopsin by carbonyl 13C‐NMR with sequence‐specific assignments , 1993, FEBS letters.
[147] S. Opella,et al. Weak alignment of membrane proteins in stressed polyacrylamide gels. , 2004, Journal of magnetic resonance.
[148] D. Nolde,et al. Three-dimensional structure of proteolytic fragment 163-231 of bacterioopsin determined from nuclear magnetic resonance data in solution. , 1992, European journal of biochemistry.
[149] S. So,et al. NMR structure of the thromboxane A2 receptor ligand recognition pocket. , 2004, European journal of biochemistry.
[150] D. Cafiso,et al. Spectroscopic evidence that osmolytes used in crystallization buffers inhibit a conformation change in a membrane protein. , 2003, Biochemistry.
[151] L. Kay,et al. Methyl Groups as Probes of Structure and Dynamics in NMR Studies of High‐Molecular‐Weight Proteins , 2005, Chembiochem : a European journal of chemical biology.
[152] L. Kay,et al. New developments in isotope labeling strategies for protein solution NMR spectroscopy. , 2000, Current opinion in structural biology.
[153] R. Leatherbarrow,et al. Effect of osmolytes on the exchange rates of backbone amide protons in proteins. , 1998, Biochemistry.
[154] D. D. Thomas,et al. Mapping the interaction surface of a membrane protein: unveiling the conformational switch of phospholamban in calcium pump regulation. , 2005, Proceedings of the National Academy of Sciences of the United States of America.
[155] J. P. Walsh,et al. sn-1,2-Diacylglycerol kinase of Escherichia coli. Structural and kinetic analysis of the lipid cofactor dependence. , 1986, Journal of Biological Chemistry.
[156] Heinz Rüterjans,et al. High level cell-free expression and specific labeling of integral membrane proteins. , 2004, European journal of biochemistry.
[157] I. Bertini,et al. Paramagnetic probes in metalloproteins. , 2001, Methods in enzymology.
[158] D. Engelman,et al. Lipid bilayer thickness varies linearly with acyl chain length in fluid phosphatidylcholine vesicles. , 1983, Journal of molecular biology.
[159] A. Bax,et al. Relaxation-optimized NMR spectroscopy of methylene groups in proteins and nucleic acids. , 2004, Journal of the American Chemical Society.
[160] Che Ma,et al. Lanthanide ions bind specifically to an added "EF-hand" and orient a membrane protein in micelles for solution NMR spectroscopy. , 2000, Journal of magnetic resonance.
[161] A. Bax,et al. Structure and orientation of a G protein fragment in the receptor bound state from residual dipolar couplings. , 2002, Journal of molecular biology.
[162] C. Sanders,et al. Membrane protein preparation for TROSY NMR screening. , 2005, Methods in enzymology.
[163] J. Chou,et al. The structure of phospholamban pentamer reveals a channel-like architecture in membranes. , 2005, Proceedings of the National Academy of Sciences of the United States of America.
[164] Walid A Houry,et al. Quantitative NMR spectroscopy of supramolecular complexes: dynamic side pores in ClpP are important for product release. , 2005, Proceedings of the National Academy of Sciences of the United States of America.
[165] H. Hauser. Short-chain phospholipids as detergents. , 2000, Biochimica et biophysica acta.
[166] A. I. Popov,et al. Three-dimensional structure of (1-71)bacterioopsin solubilized in methanol/chloroform and SDS micelles determined by 15N-1H heteronuclear NMR spectroscopy. , 1994, European journal of biochemistry.
[167] M. Girvin,et al. Solution structure of the transmembrane H+-transporting subunit c of the F1F0 ATP synthase. , 1998, Biochemistry.
[168] G. Wider,et al. TROSY in NMR studies of the structure and function of large biological macromolecules. , 2003, Current opinion in structural biology.
[169] D. Engelman,et al. Amphipols: polymeric surfactants for membrane biology research , 2003, Cellular and Molecular Life Sciences CMLS.
[170] F. Kukita,et al. Interaction stabilizing tertiary structure of bacteriorhodopsin studied by denaturation experiments , 1995, Proteins.
[171] Volker Dötsch,et al. Evaluation of detergents for the soluble expression of α‐helical and β‐barrel‐type integral membrane proteins by a preparative scale individual cell‐free expression system , 2005 .
[172] Haruki Nakamura,et al. Structural basis of the KcsA K(+) channel and agitoxin2 pore-blocking toxin interaction by using the transferred cross-saturation method. , 2003, Structure.