Immune complexes in rheumatoid synovitis: a mixed staining immunofluorescence study.

Complexes of IgG—rheumatoid factor (RF) and IgG—β1C were demonstrated in the synovial membrane obtained from patients with rheumatoid arthritis (RA) by a mixed immunofluorescence method that shows simultaneously the two components of the complex. IgG—RF was found only in the synovia of cases with circulating RF, while IgG—β1C deposits could be detected in both seropositive and seronegative cases. These findings are discussed in connection with the pathogenesis of rheumatoid synovitis in the light of the immune complex pathogenetic mechanism.

[1]  P. Schur,et al.  Reactions of aggregated mercaptoethanol treated gamma globulin with rheumatoid factor--precipitin and complement fixation studies. , 1968, Arthritis and rheumatism.

[2]  J. Vaughan,et al.  Intracytoplasmic inclusions of immunoglobulins in rheumatoid arthritis and other diseases. , 1968, Arthritis and rheumatism.

[3]  L. Bonomo,et al.  Distribution of the anti-gamma globulin factors in the synovial membrane and other tissues in various diseases. , 1968, Annals of the rheumatic diseases.

[4]  A. Michael,et al.  Immunopathologic changes in rheumatoid arthritis synovium , 1966 .

[5]  D. Zucker‐Franklin.  The phagosomes in rheumatoid synovial fluid leukocytes: A light, a light, fluorescence, and electron microscope study , 1966 .

[6]  J. Cebra,et al.  Chromatographic purification of tetramethylrhodamine-immune globulin conjugates and their use in the cellular localization of rabbit gamma-globulin polypeptide chains. , 1965, Journal of immunology.

[7]  N. Zvaifler.  A SPECULATION ON THE PATHOGENESIS OF JOINT INFLAMMATION IN RHEUMATOID ARTHRITIS. , 1965, Arthritis and rheumatism.

[8]  M. Ziff.  Heberden Oration, 1964 * , 1965, Annals of the rheumatic diseases.

[9]  D. Mccarty,et al.  STUDIES ON THE PATHOGENESIS OF RHEUMATOID JOINT INFLAMMATION. I. THE "R.A. CELL" AND A WORKING HYPOTHESIS. , 1965, Annals of internal medicine.

[10]  G. Weissmann,et al.  LYSOSOMES, AUTOIMMUNE PHENOMENA, AND DISEASES OF CONNECTIVE TISSUE. , 1964, Lancet.

[11]  R. Heimer,et al.  INHIBITION OF COMPLEMENT FIXATION BY HUMAN SERUM. II. THE ACTIVITY OF A GAMMA-LM GLOBULIN AND RHEUMATOID FACTOR IN COMPLEMENT FIXATION REACTIONS. , 1963, Journal of immunology.

[12]  J. N. Mccormick.  An Immunofluorescence Study of Rheumatoid Factor * , 1963, Annals of the rheumatic diseases.

[13]  R. Heimer,et al.  Inhibition of complement fixation by human serum. , 1962, Journal of immunology.

[14]  J. N. Mccormick.  Use of Fluorescein-labelled Rheumatoid Factor for locating Sites of Antibody Fixation in Tissues , 1962, Nature.

[15]  S. Boyden.  THE CHEMOTACTIC EFFECT OF MIXTURES OF ANTIBODY AND ANTIGEN ON POLYMORPHONUCLEAR LEUCOCYTES , 1962, The Journal of experimental medicine.

[16]  H. Kunkel,et al.  THE LOCALIZATION OF IN VIVO BOUND COMPLEMENT IN TISSUE SECTIONS , 1962, The Journal of experimental medicine.

[17]  R. Heimer,et al.  CELLULAR ORIGIN OF RHEUMATOID FACTOR , 1959, The Journal of experimental medicine.

[18]  J. Riggs,et al.  Isothiocyanate compounds as fluorescent labeling agents for immune serum. , 1958, The American journal of pathology.