Magnetic enzyme-linked immunosorbent assay (MELISA) for determination of specific IgG in paracoccidioidomycosis.

A magnetic solid phase enzyme-linked immunosorbent assay (MELISA) for quantification of IgG antibodies to somatic and metabolic antigens of Paracoccidioides brasiliensis was developed. Activation of magnetic polyacrylamide agarose beads with concanavalin A was superior to glutaraldehyde activation, and test sensitivity was higher for somatic than for metabolic antigens. Comparative MELISA, counterimmunoelectrophoresis and erythroimmunoassay tests with sera from 33 proven cases of paracoccidioidomycosis, 14 cases of histoplasmosis and 20 normal human sera showed the MELISA could distinguish antibody levels in paracoccidioidomycosis from those in normal sera; however two sera from histoplasmosis cases cross-reacted in the MELISA. MELISA is a rapid test (5-6 h) and the results suggest it has considerable potential value for assay of anti-P. brasiliensis antibodies.

[1]  J. Guesdon,et al.  Titration of antibodies to Paracoccidioides brasiliensis by erythro-immunoassay (EIA). , 1984, Sabouraudia.

[2]  D. Warnock,et al.  Enzyme-linked immunosorbent assay and its application to the serological diagnosis of fungal infection. , 1983, Sabouraudia.

[3]  J. Guesdon,et al.  Lectin immuno tests: quantitation and titration of antigens and antibodies using lectin-antibody conjugates. , 1980, Journal of immunological methods.

[4]  J. Guesdon,et al.  Sensitive titration of antibodies and antigens using erythro-immunoassay. , 1980, Annales d'immunologie.

[5]  J. Guesdon,et al.  Coupling of Enzymes to Antibodies and Antigens , 1978 .

[6]  J. Guesdon,et al.  Magnetically responsive polyacrylamide agarose beads for the preparation of immunoabsorbents. , 1978, Journal of immunological methods.

[7]  J. Guesdon,et al.  Magnetie solid phase enzyme-immunoassay. , 1977, Immunochemistry.

[8]  A. Restrepo,et al.  Characterization of the Precipitin Bands Detected in the Immunodiffusion Test for Paracoccidioidomycosis , 1974, Applied microbiology.

[9]  D. Mf,et al.  Indirect immunofluorescent reaction for the serodiagnosis of South American blastomycosis. Standardization of the reaction and comparison of results with the complement fixation test , 1973 .

[10]  E. Drouhet,et al.  [Study of precipitating antibodies in South American blastomycosis by immunoelectrophoretic analysis of Paracoccidiodes brasiliensis antigens]. , 1970, Annales de l'Institut Pasteur.