Effects of simultaneous combined exposure to CDMA and WCDMA electromagnetic field on immune functions in rats

Abstract Purpose: Despite the importance of the immune system in defending the body against infection and cancer, little research on the possible effects of radiofrequency electromagnetic field (RF-EMF) signals on immune functions exists, and, in the case of simultaneous combined exposure of RF-EMF, to the best of our knowledge no work has been done. The aim of this study was to assess the effect of simultaneous exposure to two types of RF-EMF signals, single code division multiple access (CDMA) and wideband code division multiple access (WCDMA) signals on the immune system of rats. Materials and methods: Male Sprague-Dawley rats were exposed to RF-EMF for 45 min/day, 5 days/week for up to 8 weeks. The whole body average specific absorption rate (SAR) of CDMA or WCDMA was 2.0 W/kg. Every 2 weeks after the experiment began, 20 rats were autopsied. Blood hematology, subtype population of splenocytes and cytokine production or mRNA expressions, interleukin (IL)-6, tumor necrosis factor (TNF)-α, IL-1β, interferon (IFN)-γ and transforming growth factor (TGF)-β from the splenocytes or IL-6, TNF-α, and immunoglobulin (Ig) of IgG and IgM from blood serum, were examined. Results: The results suggest that 8-week exposure to CDMA (849 MHz) and WCDMA (1.95 GHz) RF simultaneously at 2.0 W/kg each for 45-min RF-EMF exposure (total, 4 W/kg) did not affect these immune parameters. Conclusions: The present experiments suggest that simultaneous combined exposure of CDMA and WCDMA with total SAR dose of 4.0 W/kg for 45 min/day for 8 weeks, which is a relatively high SAR level compared to the exposure levels for the human system recommended by International Commission on Non-Ionizing Radiation Protection (ICNIRP, 0.4W/kg for whole body exposure levels and 2.0 W/kg for local exposure levels of general public), did not have any detectable effects on immune function in rats.

[1]  D. Juran,et al.  Cellular telephones and risk for brain tumors: A population-based, incident case-control study , 2006, Neurology.

[2]  Sung-Ho Kim,et al.  Lack of Teratogenicity after Combined Exposure of Pregnant Mice to CDMA and WCDMA Radiofrequency Electromagnetic Fields , 2009, Radiation research.

[3]  M. Geffard,et al.  A Confirmation Study of Russian and Ukrainian Data on Effects of 2450 MHz Microwave Exposure on Immunological Processes and Teratology in Rats , 2009, Radiation research.

[4]  J. H. Merritt,et al.  Effects of 20-MHz radiofrequency radiation on rat hematology, splenic function, and serum chemistry. , 1985, Radiation research.

[5]  E. Novoselova,et al.  Microwaves and cellular immunity. II. Immunostimulating effects of microwaves and naturally occurring antioxidant nutrients. , 1999, Bioelectrochemistry and bioenergetics.

[6]  M. Di Gioacchino,et al.  Combined Effects of Electromagnetic Fields on Immune and Nervous Responses , 2007, International journal of immunopathology and pharmacology.

[7]  Marek Zmyślony,et al.  Acute exposure to 930 MHz CW electromagnetic radiation in vitro affects reactive oxygen species level in rat lymphocytes treated by iron ions , 2004, Bioelectromagnetics.

[8]  R. Pinto,et al.  Effects of In Vivo Exposure to GSM-Modulated 900 MHz Radiation on Mouse Peripheral Lymphocytes , 2003, Radiation research.

[9]  Guanghui Cao,et al.  Effect of isothermal radiofrequency radiation on cytolytic T lymphocytes , 1996, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[10]  R. Smialowicz,et al.  Microwaves (2,450 MHz) suppress murine natural killer cell activity. , 1983, Bioelectromagnetics.

[11]  E. Moros,et al.  Micronuclei in the peripheral blood and bone marrow cells of rats exposed to 2450 MHz radiofrequency radiation , 2001, International journal of radiation biology.

[12]  C. Cain,et al.  Effects of microwave exposure on the hamster immune system. I. Natural killer cell activity. , 1983, Bioelectromagnetics.

[13]  W. Wiktor-Jedrzejczak,et al.  Increase in the frequency of Fc receptor (FcR) bearing cells in the mouse spleen following a single exposure of mice to 2450 MHz microwaves. , 1977, Biomedicine / [publiee pour l'A.A.I.C.I.G.].

[14]  Jeong-Ki Pack,et al.  Lymphoma development of simultaneously combined exposure to two radiofrequency signals in AKR/J mice , 2011, Bioelectromagnetics.

[15]  Giorgio Alfonso Lovisolo,et al.  Prenatal Exposure to Non-ionizing Radiation: Effects of WiFi Signals on Pregnancy Outcome, Peripheral B-Cell Compartment and Antibody Production , 2010, Radiation research.

[16]  Ersan Odaci,et al.  Effects of prenatal exposure to a 900 MHz electromagnetic field on the dentate gyrus of rats: a stereological and histopathological study , 2008, Brain Research.

[17]  Eduardo G. Moros,et al.  Chromosome Damage and Micronucleus Formation in Human Blood Lymphocytes Exposed In Vitro to Radiofrequency Radiation at a Cellular Telephone Frequency (847.74 MHz, CDMA) , 2001, Radiation research.

[18]  James R. Jauchem,et al.  Effects of low-level radio-frequency (3kHz to 300GHz) energy on human cardiovascular, reproductive, immune, and other systems: a review of the recent literature. , 2008, International journal of hygiene and environmental health.

[19]  A. Auvinen,et al.  Mobile phone use and location of glioma: A case–case analysis , 2009, Bioelectromagnetics.

[20]  D. McRee,et al.  Genotoxicity of radiofrequency signals. I. Investigation of DNA damage and micronuclei induction in cultured human blood cells , 2002, Bioelectromagnetics.

[21]  G. B. Gajda,et al.  DNA Damage in Human Leukocytes after Acute In Vitro Exposure to a 1.9 GHz Pulse-Modulated Radiofrequency Field , 2002, Radiation research.

[22]  Olle Johansson,et al.  Combined Exposure of Peripubertal Male Rats to the Endocrine-Disrupting Compound Atrazine and Power–Frequency Electromagnetic Fields Causes Degranulation of Cutaneous Mast Cells: A New Toxic Environmental Hazard? , 2010, Archives of environmental contamination and toxicology.

[23]  Jeong-Ki Pack,et al.  One-year, simultaneous combined exposure of CDMA and WCDMA radiofrequency electromagnetic fields to rats , 2011, International journal of radiation biology.

[24]  A W Wood,et al.  Effects of mobile phone type signals on calcium levels within human leukaemic T-cells (Jurkat cells) , 2001, International journal of radiation biology.

[25]  Lennart Hardell,et al.  Mobile phones, cordless phones and the risk for brain tumours. , 2009, International journal of oncology.

[26]  Lennart Hardell,et al.  Cell phones and brain tumors: a review including the long-term epidemiologic data. , 2009, Surgical neurology.

[27]  P Conti,et al.  Effects of electromagnetic fields produced by radiotelevision broadcasting stations on the immune system of women. , 2001, The Science of the total environment.

[28]  Hyung-Do Choi,et al.  The effects of simultaneous combined exposure to CDMA and WCDMA electromagnetic fields on rat testicular function , 2012, Bioelectromagnetics.

[29]  R. Pinto,et al.  Effects of GSM-Modulated Radiofrequency Electromagnetic Fields on B-Cell Peripheral Differentiation and Antibody Production , 2006, Radiation research.

[30]  Wanda Stankiewicz,et al.  Immunotropic Influence of 900 MHz Microwave GSM Signal on Human Blood Immune Cells Activated in Vitro , 2006, Electromagnetic biology and medicine.

[31]  Leeka Kheifets,et al.  Epidemiologic Evidence on Mobile Phones and Tumor Risk: A Review , 2009, Epidemiology.

[32]  Louis N Heynick,et al.  Radiofrequency (RF) effects on blood cells, cardiac, endocrine, and immunological functions , 2003, Bioelectromagnetics.

[33]  J L Chagnaud,et al.  In vivo exposure of rats to GSM-modulated microwaves: flow cytometry analysis of lymphocyte subpopulations and of mitogen stimulation. , 1999, International journal of radiation biology.