Relationships between the genome and some phenotypical properties of Lactobacillus fermentum CECT 5716, a probiotic strain isolated from human milk

Lactobacillus fermentum CECT 5716, isolated from human milk, has immunomodulatory, anti-inflammatory, and anti-infectious properties, as revealed by several in vitro and in vivo assays, which suggests a strong potential as a probiotic strain. In this work, some phenotypic properties of L. fermentum CECT 5716 were evaluated, and the genetic basis for the obtained results was searched for in the strain genome. L. fermentum CECT 5716 does not contain plasmids and showed neither bacteriocin nor biogenic amine biosynthesis ability but was able to produce organic acids, glutathione, riboflavin, and folates and to moderately stimulate the maturation of mouse dendritic cells. No prophages could be induced, and the strain was sensitive to all antibiotics proposed by European Food Safety Authority (EFSA) standards, while no transmissible genes potentially involved in antibiotic resistance were detected in its genome. Globally, there was an agreement between the phenotype properties of L. fermentum CECT 5716 and the genetic information contained in its genome.

[1]  P. Cunniff.  Official Methods of Analysis of AOAC International , 2019 .

[2]  J. M. Rodríguez,et al.  Development of a Potential Probiotic Fresh Cheese Using Two Lactobacillus salivarius Strains Isolated from Human Milk , 2014, BioMed research international.

[3]  Vineet K. Sharma,et al.  Draft Genome Sequence of Lactobacillus fermentum Lf1, an Indian Isolate of Human Gut Origin , 2013, Genome Announcements.

[4]  Christian Milani,et al.  Bacteria as vitamin suppliers to their host: a gut microbiota perspective. , 2013, Current opinion in biotechnology.

[5]  J. Garssen,et al.  Human milk: a source of more life than we imagine. , 2013, Beneficial microbes.

[6]  J. M. Rodríguez,et al.  The human milk microbiota: origin and potential roles in health and disease. , 2013, Pharmacological research.

[7]  S. Delgado,et al.  Characterization of Lactobacillus salivarius CECT 5713, a strain isolated from human milk: from genotype to phenotype , 2012, Applied Microbiology and Biotechnology.

[8]  J. Maldonado,et al.  Lactobacillus fermentum CECT 5716 is safe and well tolerated in infants of 1-6 months of age: a randomized controlled trial. , 2012, Pharmacological research.

[9]  J. Maldonado,et al.  Human Milk Probiotic Lactobacillus fermentum CECT5716 Reduces the Incidence of Gastrointestinal and Upper Respiratory Tract Infections in Infants , 2012, Journal of pediatric gastroenterology and nutrition.

[10]  P. Gunasekaran,et al.  Improvement of Riboflavin Production by Lactobacillus fermentum Isolated from Yogurt , 2011 .

[11]  F. Pérez-Cano,et al.  In vitro immunomodulatory activity of Lactobacillus fermentum CECT5716 and Lactobacillus salivarius CECT5713: two probiotic strains isolated from human breast milk. , 2010, Immunobiology.

[12]  J. M. Rodríguez,et al.  Complete Genome Sequence of Lactobacillus fermentum CECT 5716, a Probiotic Strain Isolated from Human Milk , 2010, Journal of bacteriology.

[13]  J. Xaus,et al.  Identification and evaluation of the probiotic potential of lactobacilli isolated from canine milk. , 2010, Veterinary journal.

[14]  B. Bartolomé,et al.  Antimicrobial activity of phenolic acids against commensal, probiotic and pathogenic bacteria. , 2010, Research in microbiology.

[15]  J. Xaus,et al.  Safety Assessment of Lactobacillus fermentum CECT5716, a probiotic strain isolated from human milk , 2009, Journal of Dairy Research.

[16]  J. Adrio,et al.  Metabolism of prebiotic products containing beta(2-1) fructan mixtures by two Lactobacillus strains. , 2008, Anaerobe.

[17]  M. Hattori,et al.  Comparative Genome Analysis of Lactobacillus reuteri and Lactobacillus fermentum Reveal a Genomic Island for Reuterin and Cobalamin Production , 2008, DNA research : an international journal for rapid publication of reports on genes and genomes.

[18]  M. Comalada,et al.  Evaluation of the preventative effects exerted by Lactobacillus fermentum in an experimental model of septic shock induced in mice , 2008, British Journal of Nutrition.

[19]  J. Fonollá,et al.  Oral intake of Lactobacillus fermentum CECT5716 enhances the effects of influenza vaccination. , 2007, Nutrition.

[20]  S. Roos,et al.  Antibiotic susceptibility profiles of Lactobacillus reuteri and Lactobacillus fermentum. , 2007, Journal of food protection.

[21]  J. Xaus,et al.  Two Lactobacillus strains, isolated from breast milk, differently modulate the immune response , 2007, Journal of applied microbiology.

[22]  J. Adrio,et al.  Lactobacillus fermentum, a probiotic capable to release glutathione, prevents colonic inflammation in the TNBS model of rat colitis , 2006, International Journal of Colorectal Disease.

[23]  L. Piddock.  Multidrug-resistance efflux pumps ? not just for resistance , 2006, Nature Reviews Microbiology.

[24]  J. Xaus,et al.  Antimicrobial potential of four Lactobacillus strains isolated from breast milk , 2006, Journal of applied microbiology.

[25]  J. Xaus,et al.  Characterization of a reuterin-producing Lactobacillus coryniformis strain isolated from a goat's milk cheese. , 2005, International journal of food microbiology.

[26]  Farhan M. Asrar,et al.  Bacterially synthesized folate and supplemental folic acid are absorbed across the large intestine of piglets. , 2005, The Journal of nutritional biochemistry.

[27]  F. Sesma,et al.  Lactobacillus fermentum CRL 722 is able to deliver active alpha-galactosidase activity in the small intestine of rats. , 2005, FEMS microbiology letters.

[28]  J. Xaus,et al.  Probiotic Potential of 3 Lactobacilli Strains Isolated From Breast Milk , 2005, Journal of human lactation : official journal of International Lactation Consultant Association.

[29]  M. Comalada,et al.  The intestinal anti‐inflammatory effect of quercitrin is associated with an inhibition in iNOS expression , 2004, British journal of pharmacology.

[30]  J. Xaus,et al.  Human milk is a source of lactic acid bacteria for the infant gut. , 2003, The Journal of pediatrics.

[31]  S. Connell,et al.  Ribosomal Protection Proteins and Their Mechanism of Tetracycline Resistance , 2003, Antimicrobial Agents and Chemotherapy.

[32]  Jeroen Hugenholtz,et al.  Lactobacillus reuteri CRL1098 Produces Cobalamin , 2003, Journal of bacteriology.

[33]  C. Loguercio,et al.  Glutathione supplementation improves oxidative damage in experimental colitis. , 2003, Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver.

[34]  J. Hugenholtz,et al.  Effects of Cultivation Conditions on Folate Production by Lactic Acid Bacteria , 2003, Applied and Environmental Microbiology.

[35]  M. Danielsen,et al.  Susceptibility of Lactobacillus spp. to antimicrobial agents. , 2003, International journal of food microbiology.

[36]  M. Mikelsaar,et al.  Two antioxidative lactobacilli strains as promising probiotics. , 2002, International journal of food microbiology.

[37]  A. Katla,et al.  Antimicrobial susceptibility of starter culture bacteria used in Norwegian dairy products. , 2001, International journal of food microbiology.

[38]  J. Duarte,et al.  Dietary vitamin E supplementation protects the rat large intestine from experimental inflammation. , 2001, International journal for vitamin and nutrition research. Internationale Zeitschrift fur Vitamin- und Ernahrungsforschung. Journal international de vitaminologie et de nutrition.

[39]  F. J. Romero,et al.  Replenishment of Glutathione Levels Improves Mucosal Function in Experimental Acute Colitis , 2000, Laboratory Investigation.

[40]  S. Gilliland,et al.  Comparison of newly isolated strains of Lactobacillus delbrueckii subsp. lactis for hydrogen peroxide production at 5°C. , 2000 .

[41]  Lukas Wagner,et al.  A Greedy Algorithm for Aligning DNA Sequences , 2000, J. Comput. Biol..

[42]  S. Bover-Cid,et al.  Improved screening procedure for biogenic amine production by lactic acid bacteria. , 1999, International journal of food microbiology.

[43]  P. Ducrotte,et al.  Low levels of glutathione in endoscopic biopsies of patients with Crohn's colitis: the role of malnutrition. , 1999, Clinical nutrition.

[44]  Kunitomo Watanabe,et al.  Identification of and Hydrogen Peroxide Production by Fecal and Vaginal Lactobacilli Isolated from Japanese Women and Newborn Infants , 1999, Journal of Clinical Microbiology.

[45]  P. Dudeja,et al.  Mechanism of folate transport across the human colonic basolateral membrane. , 1997, American journal of physiology. Gastrointestinal and liver physiology.

[46]  C. Forsberg,et al.  Feeding human milk to rats increases Bifidobacterium in the cecum and colon which correlates with enhanced folate status. , 1996, The Journal of nutrition.

[47]  F. Church,et al.  Spectrophotometric Assay Using o-Phthaldialdehyde for Determination of Proteolysis in Milk and Isolated Milk Proteins , 1983 .

[48]  S. Rabot,et al.  Ability of Lactobacillus fermentum to overcome host α-galactosidase deficiency , as evidenced by reduction of hydrogen excretion in rats consuming soya α-galacto-oligosaccharides , 2015 .

[49]  W. D. de Vos,et al.  The complete coenzyme B12 biosynthesis gene cluster of Lactobacillus reuteri CRL1098. , 2008, Microbiology.

[50]  J. Hugenholtz,et al.  Folate production by lactic acid bacteria and other food-grade microorganisms , 2007 .

[51]  T. Starzl,et al.  Generation of DC from mouse spleen cell cultures in response to GM-CSF: immunophenotypic and functional analyses. , 1995, Immunology.