The effect of ageing with and without non-steroidal anti-inflammatory drugs on gastrointestinal microbiology and immunology

Elderly individuals are more susceptible to gastrointestinal problems such as constipation than young adults. Furthermore, the common use of non-steroidal anti-inflammatory drugs (NSAID) among the elderly is known to further increase such gastrointestinal ailments. To describe the specific changes in elderly, intestinal microbes, their metabolites and immune markers were measured from faecal samples obtained from fifty-five elderly individuals (aged 68–88 years), using either NSAID or not, and fourteen young adults (aged 21–39 years). The faecal DM content increased with age but was significantly lower among the elderly NSAID users. The microbial metabolism was especially influenced by NSAID use and/or ageing, although fewer changes were observed in the composition of the microbial community, whilst the level of aerobes was increased in the elderly and the level of Clostridium coccoides–Eubacterium rectale reduced in the elderly NSAID users as compared with young adults. An increase in the concentrations of some branched SCFA and l-lactate but a decrease in some major SCFA concentrations were observed. Evidently, the decreased defecation frequency in the elderly directed colonic fermentation toward an unfavourable microbial metabolism but this was partially offset by the use of NSAID. Irrespective of the use of NSAID, the elderly subjects had significantly lower concentrations of faecal PGE2 than the young adults, reflecting possibly a reduced immune response. According to the present study more attention should be paid to the development of dietary products that seek to enhance bowel function, saccharolytic fermentation and immune stimulation in the elderly population.

[1]  M. Ferry,et al.  The role of nutrition in immunity in the aged. , 2009, Nutrition reviews.

[2]  J. Huneau,et al.  Effects of amino acid-derived luminal metabolites on the colonic epithelium and physiopathological consequences , 2007, Amino Acids.

[3]  N. Pace,et al.  Culture-Independent Analysis of Indomethacin-Induced Alterations in the Rat Gastrointestinal Microbiota , 2006, Applied and Environmental Microbiology.

[4]  J. Doré,et al.  Differences in Fecal Microbiota in Different European Study Populations in Relation to Age, Gender, and Country: a Cross-Sectional Study , 2006, Applied and Environmental Microbiology.

[5]  J. Wallace,et al.  Gastrointestinal Inflammation: A Central Component of Mucosal Defense and Repair , 2006, Experimental biology and medicine.

[6]  H. Harmsen,et al.  Fecal Microbiota Composition and Frailty , 2005, Applied and Environmental Microbiology.

[7]  R. Sulkava,et al.  Balancing Pain and Analgesic Treatment in the Home-Dwelling Elderly , 2005, The Annals of pharmacotherapy.

[8]  M. Mcmurdo,et al.  Comparison of Compositions and Metabolic Activities of Fecal Microbiotas in Young Adults and in Antibiotic-Treated and Non-Antibiotic-Treated Elderly Subjects , 2004, Applied and Environmental Microbiology.

[9]  P. Schwartsburd.  Age-promoted creation of a pro-cancer microenvironment by inflammation: pathogenesis of dyscoordinated feedback control , 2004, Mechanisms of Ageing and Development.

[10]  S. Salminen,et al.  New Real-Time Quantitative PCR Procedure for Quantification of Bifidobacteria in Human Fecal Samples , 2004, Applied and Environmental Microbiology.

[11]  M. Saarinen,et al.  Combination of polydextrose and lactitol affects microbial ecosystem and immune responses in rat gastrointestinal tract. , 2004, The British journal of nutrition.

[12]  H. Harmsen,et al.  Composition of faecal microbiota of elderly people , 2003 .

[13]  Dayong Wu,et al.  Ceramide-induced and Age-associated Increase in Macrophage COX-2 Expression Is Mediated through Up-regulation of NF-κB Activity* , 2003, The Journal of Biological Chemistry.

[14]  T. Schnitzer,et al.  Serious lower gastrointestinal clinical events with nonselective NSAID or coxib use. , 2003, Gastroenterology.

[15]  M. Mutanen,et al.  Culture-Independent Microbial Community Analysis Reveals that Inulin in the Diet Primarily Affects Previously Unknown Bacteria in the Mouse Cecum , 2002, Applied and Environmental Microbiology.

[16]  J. Doré,et al.  Gastrointestinal tract and the elderly: functional foods, gut microflora and healthy ageing. , 2002, Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver.

[17]  W. D. de Vos,et al.  Molecular Monitoring of Succession of Bacterial Communities in Human Neonates , 2002, Applied and Environmental Microbiology.

[18]  M Pappagallo,et al.  Incidence, prevalence, and management of opioid bowel dysfunction. , 2001, American journal of surgery.

[19]  M. Kay Washington,et al.  Prostaglandin E2 Increases Growth and Motility of Colorectal Carcinoma Cells* , 2001, The Journal of Biological Chemistry.

[20]  G. Macfarlane,et al.  Age and disease related changes in intestinal bacterial populations assessed by cell culture, 16S rRNA abundance, and community cellular fatty acid profiles , 2001, Gut.

[21]  B. Mohajer,et al.  Eicosanoids and the small intestine. , 2000, Prostaglandins & other lipid mediators.

[22]  J. Mcghee,et al.  Mucosal vaccination and immune responses in the elderly. , 2000, Vaccine.

[23]  S. Homan,et al.  Age-Associated Differences in Immunoglobulin G1 (IgG1) and IgG2 Subclass Antibodies to Pneumococcal Polysaccharides following Vaccination , 1999, Infection and Immunity.

[24]  H. Harmsen,et al.  A 16S rRNA-targeted Probe for Detection of Lactobacilli and Enterococci in Faecal Samples by Fluorescent In Situ Hybridization , 1999 .

[25]  W. Holben,et al.  Effective Recovery of Bacterial DNA and Percent-Guanine-Plus-Cytosine-Based Analysis of Community Structure in the Gastrointestinal Tract of Broiler Chickens , 1998, Applied and Environmental Microbiology.

[26]  E. Zoetendal,et al.  Temperature Gradient Gel Electrophoresis Analysis of 16S rRNA from Human Fecal Samples Reveals Stable and Host-Specific Communities of Active Bacteria , 1998, Applied and Environmental Microbiology.

[27]  Gerwin C. Raangs,et al.  Variations of Bacterial Populations in Human Feces Measured by Fluorescent In Situ Hybridization with Group-Specific 16S rRNA-Targeted Oligonucleotide Probes , 1998, Applied and Environmental Microbiology.

[28]  S. Lewis,et al.  Increasing butyrate concentration in the distal colon by accelerating intestinal transit , 1997, Gut.

[29]  J. Avorn,et al.  Bowel habit in relation to age and gender. Findings from the National Health Interview Survey and clinical implications. , 1996, Archives of internal medicine.

[30]  M. Wilkinson,et al.  Quantitative fluorescence in situ hybridization of Bifidobacterium spp. with genus-specific 16S rRNA-targeted probes and its application in fecal samples , 1995, Applied and environmental microbiology.

[31]  B. Lesourd,et al.  Cell mediated immunity changes in ageing, relative importance of cell subpopulation switches and of nutritional factors. , 1994, Immunology letters.

[32]  D. Powers.  Effect of age on serum immunoglobulin G subclass antibody responses to inactivated influenza virus vaccine , 1994, Journal of medical virology.

[33]  A. Macpherson,et al.  Side effects of nonsteroidal anti-inflammatory drugs on the small and large intestine in humans. , 1993, Gastroenterology.

[34]  J. Barton,et al.  Immunosenescence and mucosal immunity: significant effects of old age on secretory IgA concentrations and intraepithelial lymphocyte counts. , 1992, Gut.

[35]  K W Heaton,et al.  Defecation frequency and timing, and stool form in the general population: a prospective study. , 1992, Gut.

[36]  T. Mitsuoka.  Bifidobacteria and their role in human health , 1990, Journal of Industrial Microbiology.

[37]  S. Konturek,et al.  Effects of E, F, and I series prostaglandins and analogues on growth of gastroduodenal mucosa and pancreas. , 1985, The American journal of physiology.

[38]  A. Bjørneklett,et al.  Short-chain fatty acids in the normal human feces. , 1984, Scandinavian journal of gastroenterology.

[39]  A. Tamm,et al.  The production of urinary phenols by gut bacteria and their possible role in the causation of large bowel cancer. , 1976, The American journal of clinical nutrition.

[40]  T. Mitsuoka,et al.  [The faecal flora of man. II. The composition of bifidobacterium flora of different age groups (author's transl)]. , 1974, Zentralblatt fur Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene. Erste Abteilung Originale. Reihe A: Medizinische Mikrobiologie und Parasitologie.

[41]  W. E. W. ROEDlGER.  Role of anaerobic bacteria in the metabolic welfare of the colonic mucosa in man , 2006 .

[42]  M. Mcmurdo,et al.  Microbiological effects of consuming a synbiotic containing Bifidobacterium bifidum, Bifidobacterium lactis, and oligofructose in elderly persons, determined by real-time polymerase chain reaction and counting of viable bacteria. , 2005, Clinical infectious diseases : an official publication of the Infectious Diseases Society of America.

[43]  J. Newton.  Effect of Age-Related Changes in Gastric Physiology on Tolerability of Medications for Older People , 2005, Drugs & aging.

[44]  Tasuku Honjo,et al.  Intestinal IgA synthesis: regulation of front-line body defences , 2003, Nature Reviews Immunology.

[45]  J. Doré,et al.  Fusobacterium prausnitzii and related species represent a dominant group within the human fecal flora. , 2001, Systematic and applied microbiology.

[46]  H. Gill,et al.  Optimizing immunity and gut function in the elderly. , 2001, The journal of nutrition, health & aging.

[47]  Cross Ml,et al.  Optimizing immunity and gut function in the elderly. , 2001 .