Simultaneous determination of multiple (fluoro)quinolone antibiotics in food samples by a one-step fluorescence polarization immunoassay.

This paper describes a rapid one-step fluorescence polarization immunoassay (FPIA) for the simultaneous determination of multiple (fluoro)quinolone antibiotics (FQs) in food samples. Several fluorescent tracers were synthesized and evaluated in the FPIA method based on a broad-specificity of monoclonal antibodies toward FQs. The heterogeneous tracer, SAR-5-FAM, was considered as the optimal choice to prepare the immunocomplex single reagent, which allows a rapid and sensitive displacement reaction by addition of analytes. Optimized single-reagent FPIA exhibited broad cross-reactivities in the range of 7.8-172.2% with 16 FQs tested and was capable of determining most FQs at the level of maximum residue limits. Recoveries for spiked milk and chicken muscle samples were from 77.8 to 116%, with relative standard deviation lower than 17.4%. Therefore, this method could be applicable in routine screening analysis of multiple FQ residues in food samples.

[1]  R. Rickles,et al.  Simultaneous assay of Src SH3 and SH2 domain binding using different wavelength fluorescence polarization probes. , 1999, Analytical biochemistry.

[2]  M Pilar Marco,et al.  Portable surface plasmon resonance immunosensor for the detection of fluoroquinolone antibiotic residues in milk. , 2011, Journal of agricultural and food chemistry.

[3]  B. Urbaniak,et al.  Analysis of the factors that significantly influence the stability of fluoroquinolone-metal complexes. , 2009, Analytica chimica acta.

[4]  J. Brouwers,et al.  Quantitative determination of ofloxacin, ciprofloxacin, norfloxacin and pefloxacin in serum by high pressure liquid chromatography , 1986, Pharmaceutisch Weekblad.

[5]  Anton Simeonov,et al.  Fluorescence polarization assays in small molecule screening , 2011, Expert opinion on drug discovery.

[6]  R. Compañó,et al.  Analysis of flumequine and oxolinic acid in edible animal tissues by LC with fluorimetric detection , 2000 .

[7]  A. Zafra-Gómez,et al.  Simultaneous determination of 13 quinolone antibiotic derivatives in wastewater samples using solid‐phase extraction and ultra performance liquid chromatography–tandem mass spectrometry , 2013 .

[8]  Colin W. Taylor,et al.  Analysis of protein-ligand interactions by fluorescence polarization , 2011, Nature Protocols.

[9]  Qiang Zhao,et al.  Fluorescence Polarization: Recent Bioanalytical Applications, Pitfalls, and Future Trends , 2008 .

[10]  S. Walker,et al.  Discovery of O-GlcNAc transferase inhibitors. , 2005, Journal of the American Chemical Society.

[11]  Y. Picó,et al.  Development of an improved method for trace analysis of quinolones in eggs of laying hens and wildlife species using molecularly imprinted polymers. , 2012, Journal of agricultural and food chemistry.

[12]  Jungang Yin,et al.  Effect of protein on the detection of fluoroquinolone residues in fish meat. , 2012, Journal of agricultural and food chemistry.

[13]  Sergei A Eremin,et al.  Fluorescence polarization immunoassays and related methods for simple, high-throughput screening of small molecules , 2008, Analytical and bioanalytical chemistry.

[14]  D. Low,et al.  The emergence and spread of antibiotic resistance in food-borne bacteria. , 2000, International journal of food microbiology.

[15]  S. Ding,et al.  Simultaneous determination of sulphamerazine, sulphamethazine and sulphadiazine in honey and chicken muscle by a new monoclonal antibody-based fluorescence polarisation immunoassay , 2008, Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment.

[16]  Karsten Haupt,et al.  Direct fluorimetric sensing of UV-excited analytes in biological and environmental samples using molecularly imprinted polymer nanoparticles and fluorescence polarization. , 2012, Biosensors & bioelectronics.

[17]  J. Besser,et al.  Quinolone-ResistantCampylobacter jejuniInfections in Minnesota, 1992–1998 , 1999 .

[18]  Philippe Delahaut,et al.  Dual biosensor immunoassay-directed identification of fluoroquinolones in chicken muscle by liquid chromatography electrospray time-of-flight mass spectrometry. , 2007, Analytica chimica acta.

[19]  F. Sánchez-Baeza,et al.  Immunochemical assays for direct sulfonamide antibiotic detection in milk and hair samples using antibody derivatized magnetic nanoparticles. , 2008, Journal of agricultural and food chemistry.

[20]  S. Eremin,et al.  Production of Polyclonal Antibodies and Development of Fluorescence Polarization Immunoassay for Sulfanilamide , 2005 .

[21]  Andrew L. Kopp,et al.  Development and validation of a transcreener assay for detection of AMP- and GMP-producing enzymes. , 2010, Assay and drug development technologies.

[22]  Jianzhong Shen,et al.  Development of an immunochromatography strip for the rapid detection of 12 fluoroquinolones in chicken muscle and liver. , 2008, Journal of agricultural and food chemistry.

[23]  I. S. Nesterenko,et al.  Monoclonal antibody-based fluorescence polarization immunoassay for sulfamethoxypyridazine and sulfachloropyridazine. , 2007, Journal of agricultural and food chemistry.

[24]  F. Longobardi,et al.  Fluorescence polarization immunoassay for rapid screening of ochratoxin A in red wine , 2009, Analytical and bioanalytical chemistry.

[25]  C. Maragos,et al.  Fluorescence polarization as a means for determination of fumonisins in maize. , 2001, Journal of agricultural and food chemistry.

[26]  S. Eremin,et al.  Development of a rapid, specific fluorescence polarization immunoassay for the herbicide chlorsulfuron , 2002 .

[27]  Philippe Delahaut,et al.  Simultaneous determination of (fluoro)quinolone antibiotics in kidney, marine products, eggs, and muscle by enzyme-linked immunosorbent assay (ELISA). , 2006, Journal of agricultural and food chemistry.

[28]  Lei Xiang,et al.  Investigation of sulfonamide, tetracycline, and quinolone antibiotics in vegetable farmland soil in the Pearl River Delta area, southern China. , 2011, Journal of agricultural and food chemistry.

[29]  Yolanda Picó,et al.  Analytical strategies to determine quinolone residues in food and the environment , 2007 .

[30]  Daniel G. Pinacho,et al.  Molecular modeling assisted hapten design to produce broad selectivity antibodies for fluoroquinolone antibiotics. , 2012, Analytical chemistry.

[31]  Hong Wang,et al.  A simple, rapid and high-throughput fluorescence polarization immunoassay for simultaneous detection of organophosphorus pesticides in vegetable and environmental water samples. , 2011, Analytica chimica acta.

[32]  S. Eremin,et al.  Determination of chloramphenicol in milk by a fluorescence polarization immunoassay , 2010 .

[33]  S. Ding,et al.  Development of a monoclonal antibody-based broad-specificity ELISA for fluoroquinolone antibiotics in foods and molecular modeling studies of cross-reactive compounds. , 2007, Analytical chemistry.