论文信息 - A simple technique for obtaining high quality chromosome preparations from chorionic villus samples using FdU synchronization

A simple technique for obtaining high quality chromosome preparations from chorionic villus samples using FdU synchronization

A fluorordeoxyuridine (FdU) synchronization technique was applied to 30 chorionic villus samples (CVS) from patients undergoing first trimester fetal diagnosis. The villi were incubated for 15 h in the presence of FdU. The block in DNA synthesis was subsequently released using thymidine and after an additional 5 h of incubation the mitotic cells were arrested in metaphases using a high concentration of colcemid.

[1] J. Yunis,et al. New chromosome techniques in the study of human neoplasia. , 1981, Human pathology.

[2] A. Hagemeijer,et al. Improved identification of chromosomes of leukemic cells in methotrexate-treated cultures. , 1979, Cytogenetics and cell genetics.

[3] E. Flori,et al. Direct fetal chromosome studies from chorionic villi , 1985, Prenatal diagnosis.

[4] W. Rosenkranz,et al. Rapid banding technique for human and mammalian chromosomes. , 1972, Lancet.

[5] O. Garson,et al. Fluorodeoxyuridine synchronization of bone marrow cultures. , 1983, Cancer genetics and cytogenetics.

[6] M. Seabright,et al. HANDLING CHORIONIC VILLI FOR DIRECT CHROMOSOME STUDIES , 1983, The Lancet.