Genetic Diversity in the Circumsporozoite Protein Gene of Plasmodium falciparum from Major Endemic Regions of Iran

Circumsporozoite protein (CSP) is one of the stage specific antigens, which is used for the development of vaccines and to investigate the genetic diversity in Plasmodium falciparum malaria parasite. Polymerase chain reaction was used for typing of CSP genes on 67 positive falciparum malaria patients from Sistan and Baluchistan Province of Iran. Three fragments were detected for CSP gene. Twenty, 38 and 4 samples showed 700, 750 and 800 bp fragments, respectively. Sequences of some samples were aligned and compared with P.falciparum csp gene in gene bank. While the falciparum malaria endemic region of Iran is classified in low to moderate group but, extensive polymorphism was observed in the samples that could be taken into account in designing malaria vaccine.

[1]  M. Rokni,et al.  Genetic diversity in merozoite surface protein (MSP)-1 and MSP-2 genes of Plasmodium falciparum in a major endemic region of Iran. , 2007, The Korean journal of parasitology.

[2]  M. Mota,et al.  Malaria Vaccines: Back to the Future? , 2005, Science.

[3]  A. Kaneko,et al.  Stable SNPs in Malaria Antigen Genes in Isolated Populations , 2004, Science.

[4]  F. Migot-Nabias,et al.  Polymorphism in two merozoite surface proteins of Plasmodium falciparum isolates from Gabon , 2003, Malaria Journal.

[5]  F. Rakhshani,et al.  Knowledge, perceptions and prevention of malaria among women in Sistan va Baluchestan, Islamic Republic of Iran. , 2003, Eastern Mediterranean health journal = La revue de sante de la Mediterranee orientale = al-Majallah al-sihhiyah li-sharq al-mutawassit.

[6]  A. Cadwgan,et al.  Malaria vaccines: where are we and where are we going? , 2002, The Lancet. Infectious diseases.

[7]  V. Robert,et al.  Genetic diversity and population structure of Plasmodium falciparum isolates from Dakar, Senegal, investigated from microsatellite and antigen determinant loci. , 2002, Microbes and infection.

[8]  A. Moody.  Rapid Diagnostic Tests for Malaria Parasites , 2002, Clinical Microbiology Reviews.

[9]  N. White.  WHO Expert Committee on Malaria. Twentieth report: Geneva: World Health Organization, Technical Report Series no. 892, 2000. v + 71pp. Price Sw. fr. 14.-/US$12.60 (in developing countries Sw. fr. 9.80). ISBN 92-4-120892-9. Available in English; French and Spanish in preparation , 2001 .

[10]  R. Madhubala,et al.  Plasmodium falciparum: detection and strain identification of Indian isolates by polymerase chain reaction. , 2000, The Southeast Asian journal of tropical medicine and public health.

[11]  Y. D. Sharma,et al.  Genetic polymorphism of falciparum malaria vaccine candidate antigen genes among field isolates in India. , 1999, The American journal of tropical medicine and hygiene.

[12]  G. Snounou,et al.  A clonal Plasmodium falciparum population in an isolated outbreak of malaria in the Republic of Cabo Verde , 1999, Parasitology.

[13]  S. Hoffman,et al.  Immunogenicity and in vitro protective efficacy of a recombinant multistage Plasmodium falciparum candidate vaccine. , 1999, Proceedings of the National Academy of Sciences of the United States of America.

[14]  WHO Regional Office for the Eastern Mediterranean , 1995 .

[15]  G. Edrissian,et al.  Assessment of the response in vivo and in vitro of Plasmodium falciparum to sulphadoxine-pyrimethamine in the malarious areas of Iran. , 1993, The Journal of tropical medicine and hygiene.

[16]  C. Sibley,et al.  Plasmodium falciparum: a simple polymerase chain reaction method for differentiating strains. , 1992, Experimental parasitology.

[17]  T. McCutchan,et al.  Heterogeneity at the 5' end of the circumsporozoite protein gene of Plasmodium falciparum is due to a previously undescribed repeat sequence. , 1986, Nucleic acids research.

[18]  J. Weber,et al.  Structure of the gene encoding the immunodominant surface antigen on the sporozoite of the human malaria parasite Plasmodium falciparum. , 1984, Science.

[19]  L. Hviid.  NATURALLY ACQUIRED IMMUNITY TO P.FALCIPARUM MALARIA IN AFRICA , 2005 .

[20]  F. Mahboudi,et al.  Do mutations in Plasmodium falciparum dihydropteroate synthase and dihydrofolate reductase confer resistance to sulfadoxine-pyrimethamine in Iran? , 2002, Transactions of the Royal Society of Tropical Medicine and Hygiene.

[21]  S. Kariuki,et al.  A STUDY OF GENETIC DIVERSITY IN THE GENE ENCODING THE CIRCUMSPOROZOITE PROTEIN (CSP) OF P.FALCIPARUM FROM DIFFERENT TRANSMISSION AREA , 2002 .

[22]  F. Ayala,et al.  POPULATION STRUCTURE AND RECENT EVOLUTION OF P.FALCIPARUM , 2000 .

[23]  T. McCutchan,et al.  ANTIGENIC DIVERSITY IN P.FALCIPARUM , 1988 .