The traditional immobilized biomolecule detection technique on arrays has been fluorescence, but this has limitations because of its reliance on fluorophores. Alternatively, many label-free detection techniques have been developed such as surface plasmon resonance (SPR), ellipsometry, thin-film interference, diffractometry, and waveguides. These techniques have the advantage of high speed and high sensitivity, but in exchange rely on complicated structures or detailed sensing cells. A novel reflectivity-difference biosensor is introduced in this paper. When the sensor covered with a protein layer, the cells and the land would have opposite changes of the reflectance which lead to intensity shifts at the far-field diffraction plane. Therefore, it is convenient for the operation without intricate sensor structures and multifarious optical systems.
[1]
Feng Ying,et al.
Theoretical model and calibration experiment for BioCD
,
2009,
International Conference on Optical Instruments and Technology.
[2]
D. Nolte,et al.
Common-path interferometric detection of protein monolayer on the BioCD.
,
2007,
Applied optics.
[3]
Anbo Wang,et al.
Microgap Multicavity Fabry–Pérot Biosensor
,
2007,
Journal of Lightwave Technology.
[4]
J E Heebner,et al.
Sensitive disk resonator photonic biosensor.
,
2001,
Applied optics.
[5]
David D. Nolte,et al.
A novel concept for protein microarray: land-contrast BioCD
,
2009,
BiOS.