The mating factor response pathway regulates transcription of TEC1, a gene involved in pseudohyphal differentiation of Saccharomyces cerevisiae

The transcription factor Tec1 is involved in pseudohyphal differentiation and agar‐invasive growth of Saccharomyces cerevisiae cells. The sole element in the TEC1 promoter that has thus far been shown to control Tec1 function is the filament response element. We find that the TEC1 promoter also contains several pheromone response element sequences which are likely to be functional: TEC1 transcription is induced by mating factor, cell cycle regulated and dependent on the Ste4, Ste18 and Ste5 components of the mating factor signal transduction pathway. Using alleles of the transcription factor Ste12 that are defective in DNA binding, transcriptional induction or cooperativity with other transcription factors, we find little correlation between TEC1 transcript levels and agar‐invasive growth.

[1]  G. Fink,et al.  FUS3 encodes a cdc2+/CDC28-related kinase required for the transition from mitosis into conjugation , 1990, Cell.

[2]  L. Hartwell,et al.  The C-terminus of the S. cerevisiae α-pheromone receptor mediates an adaptive response to pheromone , 1988, Cell.

[3]  M. Lisanti,et al.  Gpa2p, a G-protein α-Subunit, Regulates Growth and Pseudohyphal Development in Saccharomyces cerevisiae via a cAMP-dependent Mechanism* , 1997, The Journal of Biological Chemistry.

[4]  F. Cross,et al.  Negative regulation of FAR1 at the Start of the yeast cell cycle. , 1993, Genes & development.

[5]  B. Errede,et al.  Identification of a Ty1 regulatory sequence responsive to STE7 and STE12 , 1988, Molecular and cellular biology.

[6]  Gerald R. Fink,et al.  Unipolar cell divisions in the yeast S. cerevisiae lead to filamentous growth: Regulation by starvation and RAS , 1992, Cell.

[7]  David Y. Thomas,et al.  The STE4 and STE18 genes of yeast encode potential β and γ subunits of the mating factor receptor-coupled G protein , 1989, Cell.

[8]  R. Sternglanz,et al.  The Molecular and Cellular Biology of the Yeast Saccharomyces. Volume 2: Gene Expression.Elizabeth W. Jones , John R. Pringle , James R. Broach , 1994 .

[9]  G. Fink,et al.  Elements of a single MAP kinase cascade in Saccharomyces cerevisiae mediate two developmental programs in the same cell type: mating and invasive growth. , 1994, Genes & development.

[10]  G. Sprague,,et al.  Identification and regulation of a gene required for cell fusion during mating of the yeast Saccharomyces cerevisiae , 1987, Molecular and cellular biology.

[11]  I. Herskowitz,et al.  Regulation by the yeast mating-type locus of STE12, a gene required for cell-type-specific expression , 1987, Molecular and cellular biology.

[12]  J. Heitman,et al.  The MEP2 ammonium permease regulates pseudohyphal differentiation in Saccharomyces cerevisiae , 1998, The EMBO journal.

[13]  S. Kron,et al.  Budding yeast morphogenesis: signalling, cytoskeleton and cell cycle. , 1995, Current opinion in cell biology.

[14]  G. Fink,et al.  Combinatorial Control Required for the Specificity of Yeast MAPK Signaling , 1997, Science.

[15]  G. Fink,et al.  Saccharomyces cerevisiae S288C has a mutation in FLO8, a gene required for filamentous growth. , 1996, Genetics.

[16]  W. Lo,et al.  The cell surface flocculin Flo11 is required for pseudohyphae formation and invasion by Saccharomyces cerevisiae. , 1998, Molecular biology of the cell.

[17]  G. Fink,et al.  Dissection of filamentous growth by transposon mutagenesis in Saccharomyces cerevisiae. , 1997, Genetics.

[18]  J. Heitman,et al.  Yeast pseudohyphal growth is regulated by GPA2, a G protein α homolog , 1997 .

[19]  B. Errede,et al.  A Ty1 cell-type-specific regulatory sequence is a recognition element for a constitutive binding factor , 1988, Molecular and cellular biology.

[20]  E. Dubois,et al.  Involvement of SRE element of Ty1 transposon in TEC1-dependent transcriptional activation. , 1994, Nucleic acids research.

[21]  J. Thorner,et al.  A putative protein kinase overcomes pheromone-induced arrest of cell cycling in S. cerevisiae , 1989, Cell.

[22]  G. Fink,et al.  Elements of the yeast pheromone response pathway required for filamentous growth of diploids. , 1993, Science.

[23]  B. Errede,et al.  Constitutive mutants of the protein kinase STE11 activate the yeast pheromone response pathway in the absence of the G protein. , 1992, Genes & development.

[24]  S. Reed,et al.  Plugging it in: signaling circuits and the yeast cell cycle. , 1996, Current opinion in cell biology.

[25]  E. Dubois,et al.  TEC1, a gene involved in the activation of Ty1 and Ty1-mediated gene expression in Saccharomyces cerevisiae: cloning and molecular analysis , 1990, Molecular and cellular biology.

[26]  F. Cross,et al.  Ste12 and Mcm1 regulate cell cycle-dependent transcription of FAR1 , 1996, Molecular and cellular biology.

[27]  S. Fields,et al.  Functional domains of the yeast STE12 protein, a pheromone-responsive transcriptional activator , 1993, Molecular and cellular biology.

[28]  F. Cross,et al.  ‘Marker Swap’ Plasmids: Convenient Tools for Budding Yeast Molecular Genetics , 1997 .

[29]  G. Fink,et al.  Ras2 signals via the Cdc42/Ste20/mitogen-activated protein kinase module to induce filamentous growth in Saccharomyces cerevisiae. , 1996, Proceedings of the National Academy of Sciences of the United States of America.

[30]  G. Fink,et al.  Two genes required for cell fusion during yeast conjugation: evidence for a pheromone-induced surface protein , 1987, Molecular and cellular biology.

[31]  G. Fink,et al.  Induction of pseudohyphal growth by overexpression of PHD1, a Saccharomyces cerevisiae gene related to transcriptional regulators of fungal development , 1994, Molecular and cellular biology.

[32]  K. Struhl,et al.  Current Protocols in Molecular Biology (New York: Greene Publishing Associates and Wiley-Interscience). Host-Range Shuttle System for Gene Insertion into the Chromosomes of Gram-negative Bacteria. , 1988 .

[33]  C. J. Gimeno,et al.  Saccharomyces cerevisiae TEC1 is required for pseudohyphal growth , 1996, Molecular microbiology.

[34]  F. Cross,et al.  G1 cyclins CLN1 and CLN2 repress the mating factor response pathway at Start in the yeast cell cycle. , 1994, Genes & development.

[35]  B. Errede,et al.  Cooperative binding interactions required for function of the Ty1 sterile responsive element , 1997, Molecular and cellular biology.