Effects of serum from preeclamptic women on prostacyclin production by human endothelial cells.

There is growing evidence that proteinuric hypertension of pregnancy (preeclampsia) is associated with endothelial dysfunction. The aim of this study was to evaluate the effects of serum from preeclamptic patients on basal and agonist-stimulated prostacyclin production by human umbilical vein endothelial cells (HUVEC) in culture and to compare these to the effects of serum from normal pregnant and nonpregnant women. During a 24 h incubation of HUVEC with 20% of preeclampsia serum, baseline prostacyclin output was significantly (P < 0.01) increased over the control groups. However, this response was attenuated by extending the exposure to 72 h. Histamine, thrombin and the calcium ionophore, A23187, all acutely increased prostacyclin production, but the increase relative to baseline levels was greatest in HUVEC preincubated for 24 h in normal serum transiently promotes prostacyclin production in HUVEC derived from normal pregnancies, preeclampsia serum transiently promotes prostacyclin production in HUVEC derived from normal pregnancies, and 2) the relative increase in response to agonists is reduced by preeclampsia serum, compared to normal pregnancy sera.

[1]  J. Roberts,et al.  Mechanical stress eliminates the effects of plasma from patients with preeclampsia on endothelial cells. , 1996, American journal of obstetrics and gynecology.

[2]  J. Whitworth,et al.  Preeclampsia: the effects of serum on endothelial cell prostacyclin, endothelin, and cell membrane integrity. , 1996, American journal of obstetrics and gynecology.

[3]  Tamás Suto,et al.  IMPORTANCE OF NITRIC OXIDE IN CONTROL OF SYSTEMIC AND RENAL HEMODYNAMICS DURING NORMAL PREGNANCY STUDIES IN THE RAT AND IMPLICATIONS FOR PREECLAMPSIA , 1996 .

[4]  J. Roberts,et al.  Plasma of preeclamptic women stimulates and then inhibits endothelial prostacyclin. , 1996, Hypertension.

[5]  E. Gallery,et al.  Effect of serum on secretion of prostacyclin and endothelin-1 by decidual endothelial cells from normal and preeclamptic pregnancies. , 1995, American journal of obstetrics and gynecology.

[6]  J. Roberts,et al.  Plasma from women with preeclampsia increases endothelial cell nitric oxide production. , 1995, Hypertension.

[7]  C. D. de Groot,et al.  Plasma from preeclamptic women increases human endothelial cell prostacyclin production without changes in cellular enzyme activity or mass. , 1995, American journal of obstetrics and gynecology.

[8]  G. Rice,et al.  Plasma type II phospholipase A2 levels are elevated in severe preeclampsia. , 1995, American journal of obstetrics and gynecology.

[9]  M. Molnár,et al.  Prolonged blockade of nitric oxide synthesis in gravid rats produces sustained hypertension, proteinuria, thrombocytopenia, and intrauterine growth retardation , 1994 .

[10]  C. Yallampalli,et al.  Inhibition of nitric oxide synthesis in rats during pregnancy produces signs similar to those of preeclampsia. , 1993, American journal of obstetrics and gynecology.

[11]  A. Deng,et al.  Chronic blockade of nitric oxide synthesis in the rat produces systemic hypertension and glomerular damage. , 1992, The Journal of clinical investigation.

[12]  A. Goldfien,et al.  Sera From Preeclamptic Women Specifically Activate Human Umbilical Vein Endothelial Cells In Vitro: Morphological and Biochemical Evidence , 1992, American journal of reproductive immunology.

[13]  J. Whitworth,et al.  Endothelium-derived prostacyclin: effect of serum from women with normal and hypertensive pregnancy. , 1992, Clinical science.

[14]  M. Mitchell,et al.  Sera from preeclamptic patients contain factor(s) that stimulate prostacyclin production by human endothelial cells. , 1992, Prostaglandins, leukotrienes, and essential fatty acids.

[15]  T. Henriksen,et al.  Sera from preeclamptic women increase the content of triglycerides and reduce the release of prostacyclin in cultured endothelial cells. , 1991, Thrombosis research.

[16]  J. Vane,et al.  Endothelium-derived vasoactive factors and the control of the circulation. , 1991, Seminars in perinatology.

[17]  R. W. Kuhn,et al.  Partial characterization of a novel growth factor from the blood of women with preeclampsia. , 1990, The Journal of clinical endocrinology and metabolism.

[18]  T. Musci,et al.  Preeclampsia: an endothelial cell disorder. , 1989, American journal of obstetrics and gynecology.

[19]  S. Walsh,et al.  Preeclampsia: an imbalance in placental prostacyclin and thromboxane production. , 1985, American journal of obstetrics and gynecology.

[20]  P. Lewis,et al.  REDUCED PROSTACYCLIN PRODUCTION IN PRE-ECLAMPSIA , 1980, The Lancet.

[21]  H. Behrman,et al.  2 – Prostaglandins and Prostaglandin Metabolites , 1978 .

[22]  E. Jaffe,et al.  Culture of human endothelial cells derived from umbilical veins. Identification by morphologic and immunologic criteria. , 1973, The Journal of clinical investigation.