Comparison of estimates of gonadotropin levels by isolated blood samples, integrated blood concentrations, and timed urinary fractions.

Gonadotropin levels in isolated blood samples, integrated plasma concentrations (IC), and timed urinary collections have been compared in 5 males with delayed puberty and 7 normal adult males. There was a significant correlation between urinary levels in 24-h collection and those in each of four shorter timed collections for both LH and FSH. Similarly, 24-h integrated plasma concentration and 4-h (0800--1200 h) integrated plasma concentration obtained on 10 additional subjects showed significant correlation. The 4-h integrated plasma concentrations correlated with single blood samples or the mean of three samples obtained at 0800, 1200, and 1600 h. These 4-h plasma samples also correlated significantly with all urine collections for FSH but only with the 2200--0800 h urine collection for LH. The study suggests that LH and FSH levels in urine samples collected over several hours correlate with 24-h urinary excretion and that levels in single blood samples estimate the 24-h plasma integrated concentration.

[1]  P. A. Lee,et al.  Integrated concentrations of follicle stimulating hormone and puberty. , 1978, The Journal of clinical endocrinology and metabolism.

[2]  H. Kulin,et al.  Timed urinary gonadotropin measurements in normal infants, children, and adults, and in patients with disorders of sexual maturation. , 1977, The Journal of pediatrics.

[3]  R. Penny,et al.  Overnight follicle stimulating hormone (FSH) and luteinizing hormone (LH) excretion in normal males. , 1976, The Journal of clinical endocrinology and metabolism.

[4]  P. A. Lee,et al.  Integrated concentrations of luteinizing hormone and puberty. , 1976, The Journal of clinical endocrinology and metabolism.

[5]  R. Santen,et al.  Integration of pulsatile gonadotropin secretion by timed urinary measurements: an accurate and sensitive 3-hour test. , 1975, The Journal of clinical endocrinology and metabolism.

[6]  H. Roffwarg,et al.  Human puberty. Simultaneous augmented secretion of luteinizing hormone and testosterone during sleep. , 1974, The Journal of clinical investigation.

[7]  R. Santen,et al.  EPISODIC LUTEINIZING HORMONE SECRETION IN MAN , 1974 .

[8]  H. Kulin,et al.  Preparation of urine containing small amounts of FSH and LH for radioimmunoassay: comparison of the kaolin-acetone and acetone extraction techniques. , 1973, The Journal of clinical endocrinology and metabolism.

[9]  S. Kapen,et al.  Synchronization of augmented luteinizing hormone secretion with sleep during puberty. , 1972, The New England journal of medicine.

[10]  S. Kapen,et al.  Twenty-four hour pattern of luteinizing hormone secretion in normal men with sleep stage recording. , 1972, The Journal of clinical endocrinology and metabolism.

[11]  P Troen,et al.  Repetitive luteinizing hormone elevations in serum of normal men. , 1971, The Journal of clinical endocrinology and metabolism.

[12]  R. Blizzard,et al.  Immunoreactive luteinizing hormone in acetone precipitates and kaolin extracts of human urine. , 1970, American journal of obstetrics and gynecology.

[13]  C. Migeon,et al.  Serum luteinizing hormone by radioimmunoassay in normal children. , 1969, The Journal of pediatrics.

[14]  Y. D. Coble,et al.  Production rates and metabolic clearance rates of human follicle-stimulating hormone in premenopausal and postmenopausal women. , 1969, The Journal of clinical investigation.

[15]  G. Ross,et al.  Radioimmunoassay for human luteinizing hormone. , 1966, Metabolism: clinical and experimental.