Photobioreactors for Mass Production of Microalgae

Abstract Microalgae have been proposed as valuable microorganisms for several applications, from production of pharmaceuticals to wastewater treatment. Whatever the final application, the core of the process is the photobioreactor in which microalgae are produced. To design adequate photobioreactors, it is mandatory to understand the major phenomena limiting the performance of microalgae cells such as light availability, nutrients supply including CO2, environmental conditions including temperature and solar radiation, and mixing. To fulfill the requirements of microalgae cells, different technologies has been proposed such as raceway and thin-layer open reactors, in addition to tubular and flat-plate closed reactors. These technologies are still being upgraded and improved to maximize the biomass production capacity and to reduce the production cost. Additionally, the control and modeling of these reactors is a hot topic for the industrial development of microalgae-based processes. This chapter summarizes the current state of the art on photobioreactor design and operation, discussing the major challenges to be solved to achieve a massive expansion of microalgae-based technologies.

[1]  Scott C. James,et al.  Modeling Algae Growth in an Open-Channel Raceway , 2008, J. Comput. Biol..

[2]  J. M. Fernández-Sevilla,et al.  Biotechnological production of lutein and its applications , 2010, Applied Microbiology and Biotechnology.

[3]  Y. Chisti,et al.  Photobioreactor scale-up for a shear-sensitive dinoflagellate microalga , 2011 .

[4]  Norbert Gerbsch,et al.  Monoseptic cultivation of phototrophic microorganisms--development and scale-up of a photobioreactor system with thermal sterilization. , 2003, Biomolecular engineering.

[5]  A. Shilton,et al.  Wastewater treatment high rate algal ponds for biofuel production. , 2011, Bioresource technology.

[6]  Q. Hu,et al.  Ultrahigh-cell-density culture of a marine green alga Chlorococcum littorale in a flat-plate photobioreactor , 1998, Applied Microbiology and Biotechnology.

[7]  G. C. Zittelli,et al.  Advances in microalgal culture for aquaculture feed and other uses , 2009 .

[9]  J. Doucha,et al.  Utilization of flue gas for cultivation of microalgae Chlorella sp.) in an outdoor open thin-layer photobioreactor , 2005, Journal of Applied Phycology.

[10]  L. Rodolfi,et al.  Microalgae for oil: Strain selection, induction of lipid synthesis and outdoor mass cultivation in a low‐cost photobioreactor , 2009, Biotechnology and bioengineering.

[11]  C. Posten,et al.  Second Generation Biofuels: High-Efficiency Microalgae for Biodiesel Production , 2008, BioEnergy Research.

[12]  P. Holland,et al.  Optimization of growth and production of toxins by three dinoflagellates in photobioreactor cultures , 2011, Journal of Applied Phycology.

[13]  E. Grima,et al.  Prediction of dissolved oxygen and carbon dioxide concentration profiles in tubular photobioreactors for microalgal culture , 1999, Biotechnology and bioengineering.

[14]  F. G. Acién,et al.  Characterization of a flat plate photobioreactor for the production of microalgae , 2008 .

[15]  A. Richmond,et al.  A new tubular reactor for mass production of microalgae outdoors , 1993, Journal of Applied Phycology.

[16]  A. Richmond Handbook of microalgal culture: biotechnology and applied phycology. , 2004 .

[17]  Olivier Bernard,et al.  Validation of a simple model accounting for light and temperature effect on microalgal growth. , 2012, Bioresource technology.

[18]  J. M. Fernández-Sevilla,et al.  Comprehensive model of microalgae photosynthesis rate as a function of culture conditions in photobioreactors , 2013, Applied Microbiology and Biotechnology.

[19]  D. Stengel,et al.  Algal chemodiversity and bioactivity: sources of natural variability and implications for commercial application. , 2011, Biotechnology advances.

[20]  Ivan Málek,et al.  Dual Purpose Open Circulation Units for Large Scale Culture of Algae in Temperate Zones. I. Basic Design Considerations and Scheme of a Pilot Plant , 1970 .

[21]  Yoojeong Kim,et al.  Air-Lift Bioreactors for Algal Growth on Flue Gas: Mathematical Modeling and Pilot-Plant Studies , 2005 .

[22]  Chun-Chong Fu,et al.  Effects of using light-emitting diodes on the cultivation of Spirulina platensis , 2007 .

[23]  J. Burkholder,et al.  Pfiesteria piscicida and other Pfiesreria‐like dinoflagellates: Behavior, impacts, and environmental controls , 1997 .

[24]  F. Figueroa,et al.  Photosynthesis monitoring to optimize growth of microalgal mass cultures: application of chlorophyll fluorescence techniques , 2014 .

[25]  C. Ugwu,et al.  Photobioreactors for mass cultivation of algae. , 2008, Bioresource technology.

[26]  Bobban Subhadra,et al.  Algal biorefinery-based industry: an approach to address fuel and food insecurity for a carbon-smart world. , 2011, Journal of the science of food and agriculture.

[27]  Carlos Jiménez,et al.  The Feasibility of industrial production of Spirulina (Arthrospira) in Southern Spain , 2003 .

[28]  Cecilia Faraloni,et al.  Outdoor H₂ production in a 50-L tubular photobioreactor by means of a sulfur-deprived culture of the microalga Chlamydomonas reinhardtii. , 2012, Journal of biotechnology.

[29]  R. O. Cañizares-Villanueva,et al.  Hydrodynamic and mass transfer characterization of a flat-panel airlift photobioreactor with high light path , 2010 .

[30]  Dong Gu Choi,et al.  Life cycle energy and greenhouse gas emissions for an ethanol production process based on blue-green algae. , 2010, Environmental science & technology.

[31]  F. G. Acién,et al.  Production cost of a real microalgae production plant and strategies to reduce it. , 2012, Biotechnology advances.

[32]  Ulrike Schmid-Staiger,et al.  Optimization of eicosapentaenoic acid production byPhaeodactylum tricornutumin the flat panel airlift (FPA) reactor , 2004, Journal of Applied Phycology.

[33]  E. Molina Grima,et al.  Influence of power supply in the feasibility of Phaeodactylum tricornutum cultures , 2004, Biotechnology and bioengineering.

[34]  L. Nedbal,et al.  Variation in some photosynthetic characteristics of microalgae cultured in outdoor thin-layered sloping reactors , 1995, Journal of Applied Phycology.

[35]  P. Carlozzi Closed Photobioreactor Assessments to Grow, Intensively, Light Dependent Microorganisms: A Twenty-Year Italian Outdoor Investigation , 2008 .

[36]  Giuseppe Torzillo,et al.  In situ monitoring of chlorophyll fluorescence to assess the synergistic effect of low temperature and high irradiance stresses inSpirulina cultures grown outdoors in photobioreactors , 1996, Journal of Applied Phycology.

[37]  J. W. Zijffers The green solar collector: optimization of microalgal areal productivity , 2009 .

[38]  Philip Owende,et al.  Biofuels from microalgae—A review of technologies for production, processing, and extractions of biofuels and co-products , 2010 .

[39]  A. Vonshak,et al.  Photoadaptation, photoinhibition and productivity in the blue‐green alga, Spirulina platensis grown outdoors , 1992 .

[40]  Emilio Molina,et al.  Influence of culture conditions on the productivity and lutein content of the new strain Scenedesmus almeriensis , 2008 .

[41]  Maria J Barbosa,et al.  Microalgal production--a close look at the economics. , 2011, Biotechnology advances.

[42]  G. C. Zittelli,et al.  A vertical alveolar panel (VAP) for outdoor mass cultivation of microalgae and cyanobacteria , 1991 .

[43]  Navid R. Moheimani,et al.  Sustainable biofuels from algae , 2013, Mitigation and Adaptation Strategies for Global Change.

[44]  E. Belarbi,et al.  New Culture Approaches for Yessotoxin Production from the Dinoflagellate Protoceratium reticulatum , 2007, Biotechnology progress.

[45]  N. T. Eriksen The technology of microalgal culturing , 2008, Biotechnology Letters.

[46]  Jo‐Shu Chang,et al.  Cultivation, photobioreactor design and harvesting of microalgae for biodiesel production: a critical review. , 2011, Bioresource technology.

[47]  H Guterman,et al.  A flat inclined modular photobioreactor for outdoor mass cultivation of photoautotrophs , 2000, Biotechnology and bioengineering.

[48]  Paola Maria Pedroni,et al.  Enitecnologie R&D project on microalgae biofixation of CO2: Outdoor comparative tests of biomass productivity using flue gas CO2 from a NGCC power plant , 2005 .

[49]  John R. Benemann,et al.  Microalgae for Biofuels and Animal Feeds , 2013 .

[50]  M. Eppink,et al.  Microalgae for the production of bulk chemicals and biofuels , 2010 .

[51]  O. Pulz,et al.  IGV GmbH Experience Report, Industrial Production of Microalgae Under Controlled Conditions: Innovative Prospects , 2013 .

[52]  O. Pulz,et al.  Photobioreactors: production systems for phototrophic microorganisms , 2001, Applied Microbiology and Biotechnology.

[53]  Mario R Tredici,et al.  Growth medium recycling in Nannochloropsis sp. mass cultivation. , 2003, Biomolecular engineering.

[54]  R. Buchholz,et al.  Microalgae as natural sources for antioxidative compounds , 2011, Journal of Applied Phycology.

[55]  A.J.B. van Boxtel,et al.  Scenario analysis of large scale algae production in tubular photobioreactors , 2013 .

[56]  H. Saiki,et al.  Investigation of photobioreactor design for enhancing the photosynthetic productivity of microalgae. , 2000, Biotechnology and bioengineering.

[57]  Mark A. White,et al.  Environmental life cycle comparison of algae to other bioenergy feedstocks. , 2010, Environmental science & technology.

[58]  E. Molina Grima,et al.  A mathematical model of microalgal growth in light-limited chemostat culture , 1994 .

[59]  Carlos Vílchez,et al.  Horizontal or vertical photobioreactors? How to improve microalgae photosynthetic efficiency. , 2011, Bioresource technology.

[60]  Hadiyanto,et al.  Overcoming shear stress of microalgae cultures in sparged photobioreactors , 2004, Biotechnology and bioengineering.

[61]  R. Muñoz,et al.  Biofilm photobioreactors for the treatment of industrial wastewaters. , 2009, Journal of hazardous materials.

[62]  F. Marquez,et al.  Inhibitory effect of oxygen accumulation on the growth of Spirulina platensis , 1995, Biotechnology Letters.

[63]  U. Schmid-Staiger,et al.  A novel airlift photobioreactor with baffles for improved light utilization through the flashing light effect. , 2001, Journal of biotechnology.

[64]  Yusuf Chisti,et al.  Raceways-based Production of Algal Crude Oil , 2013 .

[65]  P. Jaouen,et al.  Effects of shear on two microalgae species. Contribution of pumps and valves in tangential flow filtration systems. , 1999, Biotechnology and bioengineering.

[66]  L. Tabil,et al.  Experimental trials to make wheat straw pellets with wood residue and binders. , 2014 .

[67]  F. G. Acién,et al.  Fluid-dynamic characterization of real-scale raceway reactors for microalgae production , 2013 .

[68]  M. Borowitzka,et al.  Production of biofuels from microalgae , 2011, Mitigation and Adaptation Strategies for Global Change.

[69]  C. Aflalo,et al.  On the relative efficiency of two- vs. one-stage production of astaxanthin by the green alga Haematococcus pluvialis. , 2007, Biotechnology and bioengineering.

[70]  B. Palsson,et al.  High‐density algal photobioreactors using light‐emitting diodes , 1994, Biotechnology and bioengineering.

[71]  A. Richmond,et al.  Quantitative assessment of the major limitations on productivity ofSpirulina platensis in open raceways , 1990, Journal of Applied Phycology.

[72]  John S. Burlew,et al.  Algal culture from laboratory to pilot plant. , 1953 .

[73]  D. Singh,et al.  Photooxidative damage to the cyanobacterium Spirulina platensis mediated by singlet oxygen , 1995, Current Microbiology.

[74]  R. Wijffels,et al.  An Outlook on Microalgal Biofuels , 2010, Science.

[75]  G. C. Zittelli,et al.  Efficiency of sunlight utilization: tubular versus flat photobioreactors , 1998, Biotechnology and bioengineering.

[76]  David Chiaramonti,et al.  Review of energy balance in raceway ponds for microalgae cultivation: Re-thinking a traditional system is possible , 2013 .

[77]  J. Doucha,et al.  Outdoor open thin-layer microalgal photobioreactor: potential productivity , 2009, Journal of Applied Phycology.

[78]  Mathieu Streefland,et al.  Photosynthetic efficiency of Chlamydomonas reinhardtii in flashing light , 2011, Biotechnology and bioengineering.

[79]  R. Eppley,et al.  Predicting production in light-limited continuous cultures of algae. , 1965, Applied microbiology.

[80]  C. Anbazhagan,et al.  Microalgae: a sustainable feed source for aquaculture , 2011 .

[81]  Outdoor pilot-scale production of Botryococcus braunii in panel reactors , 2012, Journal of Applied Phycology.

[82]  S. Miyachi,et al.  Evaluation of a vertical flat-plate photobioreactor for outdoor biomass production and carbon dioxide bio-fixation: effects of reactor dimensions, irradiation and cell concentration on the biomass productivity and irradiation utilization efficiency , 2001, Applied Microbiology and Biotechnology.

[83]  S. Wood,et al.  Harnessing the self-harvesting capability of benthic cyanobacteria for use in benthic photobioreactors , 2011, AMB Express.

[84]  F. G. Fernández,et al.  Photolimitation and photoinhibition as factors determining optimal dilution rate to produce eicosapentaenoic acid from cultures of the microalga Isochrysis galbana , 1998, Applied Microbiology and Biotechnology.

[85]  P. Harrison,et al.  Acclimation and toxicity of high ammonium concentrations to unicellular algae. , 2014, Marine pollution bulletin.

[86]  E. Molina Grima,et al.  Conversion of CO2 into biomass by microalgae: how realistic a contribution may it be to significant CO2 removal? , 2012, Applied Microbiology and Biotechnology.

[87]  Patrick E. Wiley,et al.  Research Spotlight: The future of biofuels: is it in the bag? , 2012 .

[88]  H. Guterman,et al.  A macromodel for outdoor algal mass production , 1990, Biotechnology and bioengineering.

[89]  J. C. Goldman,et al.  Outdoor algal mass cultures—II. Photosynthetic yield limitations☆ , 1979 .

[90]  R. Sims,et al.  Rotating algal biofilm reactor and spool harvester for wastewater treatment with biofuels by‐products , 2012, Biotechnology and bioengineering.

[91]  Dipl.-Biotechnol Anna Jacobi,et al.  Photobioreactors: Hydrodynamics and mass transfer , 2010 .

[92]  F. G. Acién,et al.  Tubular photobioreactor design for algal cultures. , 2001, Journal of biotechnology.

[93]  F. G. Acién,et al.  Oxygen transfer and evolution in microalgal culture in open raceways. , 2013, Bioresource technology.

[94]  J. C. Merchuk,et al.  Modeling of photobioreactors: Application to bubble column simulation , 2003, Journal of Applied Phycology.

[95]  J. Costa,et al.  Pilot scale semicontinuous production of Spirulina biomass in southern Brazil. , 2009 .

[96]  Appraisal of a horizontal two-phase flow photobioreactor for industrial production of delicate microalgae species , 2012, Journal of Applied Phycology.

[97]  Kai Zhang,et al.  Photosynthetic performance of a cyanobacterium in a vertical flat-plate photobioreactor for outdoor microalgal production and fixation of CO2 , 2004, Biotechnology Letters.

[98]  Jorge Alberto Vieira Costa,et al.  Optimization of the repeated batch cultivation of microalga Spirulina platensis in open raceway ponds , 2007 .

[99]  Y. Chisti,et al.  Airlift-driven external-loop tubular photobioreactors for outdoor production of microalgae: assessment of design and performance , 2001 .

[100]  Arnaud Hélias,et al.  Life-cycle assessment of biodiesel production from microalgae. , 2009, Environmental science & technology.

[101]  Razif Harun,et al.  Bioprocess engineering of microalgae to produce a variety of consumer products , 2010 .

[102]  Mario R. Tredici,et al.  As integrated culture system for outdoor production of microalgae and cyanobacteria , 1997, Journal of Applied Phycology.

[103]  F. G. Acién,et al.  Evaluation of carbon dioxide mass transfer in raceway reactors for microalgae culture using flue gases. , 2014, Bioresource technology.

[104]  Y. Chisti,et al.  Comparative evaluation of compact photobioreactors for large-scale monoculture of microalgae , 1999 .

[105]  C. Riquelme,et al.  Comparisons of the growth of six diatom species between two configurations of photobioreactors , 2008 .

[106]  Johannes Tramper,et al.  Enclosed outdoor photobioreactors: light regime, photosynthetic efficiency, scale-up, and future prospects. , 2003, Biotechnology and bioengineering.

[107]  Miguel Olaizola,et al.  Commercial development of microalgal biotechnology: from the test tube to the marketplace. , 2003, Biomolecular engineering.

[108]  Hiroshi Saiki,et al.  Development of a photobioreactor incorporating Chlorella sp. for removal of CO2 in stack gas , 1997 .

[109]  R. Bassi,et al.  Regulation of the pigment optical density of an algal cell: filling the gap between photosynthetic productivity in the laboratory and in mass culture. , 2012, Journal of biotechnology.

[110]  Graziella Chini Zittelli,et al.  Mass cultivation of Nannochloropsis sp. in annular reactors , 2003, Journal of Applied Phycology.

[111]  Robert E. Jinkerson,et al.  Genetic Engineering of Algae for Enhanced Biofuel Production , 2010, Eukaryotic Cell.

[112]  Aditya M. Kunjapur,et al.  Photobioreactor Design for Commercial Biofuel Production from Microalgae , 2010 .

[113]  Kristina M. Weyer,et al.  Theoretical Maximum Algal Oil Production , 2009, BioEnergy Research.

[114]  G. Torzillo,et al.  A two‐plane tubular photobioreactor for outdoor culture of Spirulina , 1993, Biotechnology and bioengineering.

[115]  Giuseppe Torzillo,et al.  Production of Spirulina biomass in closed photobioreactors , 1986 .

[116]  Bryan Willson,et al.  Microalgae growth modeling and control for a vertical flat panel photobioreactor , 2009, 2009 American Control Conference.

[117]  Y. Chisti,et al.  Biotechnological significance of toxic marine dinoflagellates. , 2007, Biotechnology advances.

[118]  A. Kiperstok,et al.  Comparative energy life-cycle analyses of microalgal biomass production in open ponds and photobioreactors. , 2010, Bioresource technology.

[119]  J. Perales,et al.  Performance of a flat panel reactor in the continuous culture of microalgae in urban wastewater: prediction from a batch experiment. , 2013, Bioresource technology.

[120]  G. C. Zittelli,et al.  Effect of the Inclusion of Dried Tetraselmis suecica on Growth, Feed Utilization, and Fillet Composition of European Sea Bass Juveniles Fed Organic Diets , 2012 .

[121]  Mario R. Tredici,et al.  Photobiology of microalgae mass cultures: understanding the tools for the next green revolution , 2010 .

[122]  J. Cooney,et al.  Convenient large-scale purification of yessotoxin from Protoceratium reticulatum culture and isolation of a novel furanoyessotoxin. , 2007, Journal of agricultural and food chemistry.

[123]  F. G. Acién,et al.  Biomass and lutein productivity of Scenedesmus almeriensis: influence of irradiance, dilution rate and temperature , 2008, Applied Microbiology and Biotechnology.

[124]  J. Kopecký,et al.  Productivity correlated to photobiochemical performance of Chlorella mass cultures grown outdoors in thin-layer cascades , 2011, Journal of Industrial Microbiology & Biotechnology.

[125]  Thongchai Srinophakun,et al.  Design of raceway ponds for producing microalgae , 2012 .

[126]  P. Spolaore,et al.  Commercial applications of microalgae. , 2006, Journal of bioscience and bioengineering.

[127]  Y. Watanabe,et al.  Photosynthetic production of microalgal biomass in a raceway system under greenhouse conditions in Sendai city. , 2000, Journal of bioscience and bioengineering.

[128]  E. Molina Grima,et al.  Outdoor continuous culture of Porphyridium cruentum in a tubular photobioreactor: quantitative analy , 1999 .

[129]  R. Craggs,et al.  Hectare-scale demonstration of high rate algal ponds for enhanced wastewater treatment and biofuel production , 2012, Journal of Applied Phycology.

[130]  F. G. Acién,et al.  Effective utilization of flue gases in raceway reactor with event-based pH control for microalgae culture. , 2014, Bioresource technology.

[131]  Mario R. Tredici,et al.  Harvest of Arthrospira platensis from Lake Kossorom (Chad) and its household usage among the Kanembu , 2000, Journal of Applied Phycology.

[132]  F. G. Acién,et al.  Production of astaxanthin by Haematococcus pluvialis: taking the one-step system outdoors. , 2009, Biotechnology and bioengineering.

[133]  A. Richmond,et al.  Optimization of a flat plate glass reactor for mass production of Nannochloropsis sp. outdoors. , 2001, Journal of biotechnology.

[134]  José Luis Guzmán,et al.  Modelling and Control Issues of pH in Tubular Photobioreactors , 2010 .

[135]  A. Richmond,et al.  An industrial-size flat plate glass reactor for mass production of Nannochloropsis sp. (Eustigmatophyceae) , 2001 .

[136]  Timothy Y James,et al.  Isolation and characterization of a novel chytrid species (phylum Blastocladiomycota), parasitic on the green alga Haematococcus. , 2008, Mycological research.

[137]  Yuan-Kun Lee Enclosed bioreactors for the mass cultivation of photosynthetic microorganisms: the future trend , 1986 .

[138]  A.J.B. van Boxtel,et al.  Design scenarios for flat panel photobioreactors , 2011 .

[139]  C. O'kelly,et al.  Small doses, big troubles: modeling growth dynamics of organisms affecting microalgal production cultures in closed photobioreactors. , 2013, Bioresource technology.

[140]  A. Vonshak Spirulina: Growth, Physiology and Biochemistry , 1997 .

[141]  A. Richmond,et al.  Effect of light-path length in outdoor flat plate reactors on output rate of cell mass and of EPA in Nannochloropsis sp. , 1999 .

[142]  J. Grobbelaar,et al.  Physiological and technological considerations for optimising mass algal cultures , 2000, Journal of Applied Phycology.

[143]  Diane Thomas,et al.  Experimental characterization and numerical simulation of the hydrodynamics in an airlift photobioreactor for microalgae cultures , 2014 .

[144]  A. Cembella,et al.  Cell cycle and toxin production in the benthic dinoflagellate Prorocentrum lima , 1999 .

[145]  D. Das,et al.  Development of suitable photobioreactors for CO2 sequestration addressing global warming using green algae and cyanobacteria. , 2011, Bioresource technology.

[146]  P. Nichols,et al.  Lipid and fatty acid yield of nine stationary-phase microalgae: Applications and unusual C24–C28 polyunsaturated fatty acids , 2005, Journal of Applied Phycology.

[147]  A. Svenson,et al.  Assessment of ammonia toxicity in tests with the microalga, Nephroselmis pyriformis, Chlorophyta. , 2003, Water research.

[148]  C. Aflalo,et al.  12 Theoretical Analysis of Culture Growth in Flat-Plate Bioreactors: The Essential Role of Timescales , 2013 .

[149]  N. Kurano,et al.  Use of photoacclimation in the design of a novel photobioreactor to achieve high yields in algal mass cultivation , 2003, Journal of Applied Phycology.

[150]  Clemens Posten,et al.  Closed photo-bioreactors as tools for biofuel production. , 2009, Current opinion in biotechnology.

[151]  F. G. Fernández,et al.  Photobioreactors for the production of microalgae , 2013 .

[152]  A. Carvalho,et al.  Microalgal Reactors: A Review of Enclosed System Designs and Performances , 2006, Biotechnology progress.

[153]  F. Figueroa,et al.  Hydrodynamics and photosynthesis performance of Chlorella fusca (Chlorophyta) grown in a thin-layer cascade (TLC) system , 2014 .

[154]  V. Jirka,et al.  A two-stage solar photobioreactor for cultivation of microalgae based on solar concentrators , 2009, Journal of Applied Phycology.

[155]  C. Posten,et al.  Developments and perspectives of photobioreactors for biofuel production , 2010, Applied Microbiology and Biotechnology.

[156]  Navid Reza Moheimani,et al.  Limits to productivity of the alga Pleurochrysis carterae (Haptophyta) grown in outdoor raceway ponds , 2007, Biotechnology and bioengineering.

[157]  Johannes Tramper,et al.  Prediction of volumetric productivity of an outdoor photobioreactor , 2007, Biotechnology and bioengineering.

[158]  Daniel Chaumont,et al.  Biotechnology of algal biomass production: a review of systems for outdoor mass culture , 1993, Journal of Applied Phycology.

[159]  Y. Chisti,et al.  Photobioreactors: light regime, mass transfer, and scaleup , 1999 .

[160]  David Faiman,et al.  Optimal tilt angles of enclosed reactors for growing photoautotrophic microorganisms outdoors , 1998 .

[161]  M. Huesemann,et al.  A screening model to predict microalgae biomass growth in photobioreactors and raceway ponds , 2013, Biotechnology and bioengineering.

[162]  Jos Malda,et al.  Hydrodynamics and mass transfer in a tubular airlift photobioreactor , 2002, Journal of Applied Phycology.

[163]  Clemens Posten,et al.  Design principles of photo‐bioreactors for cultivation of microalgae , 2009 .

[164]  Wayne W. Carmichael,et al.  Harvesting of Aphanizomenon flos-aquae Ralfs ex Born. & Flah. var. flos-aquae (Cyanobacteria) from Klamath Lake for human dietary use , 2000, Journal of Applied Phycology.

[165]  I. Perner-Nochta,et al.  Photoautotrophic Cell and Tissue Culture in a Tubular Photobioreactor , 2007 .

[166]  K. G. Satyanarayana,et al.  A review on microalgae, a versatile source for sustainable energy and materials , 2011 .

[167]  Buddhi P. Lamsal,et al.  Heterotrophic/mixotrophic cultivation of oleaginous Chlorella vulgaris on industrial co-products , 2012 .

[168]  P. Carlozzi,et al.  Dilution of solar radiation through "culture" lamination in photobioreactor rows facing south-north: a way to improve the efficiency of light utilization by cyanobacteria (Arthrospira platensis). , 2003, Biotechnology and bioengineering.

[169]  P. Harrison,et al.  Recipes for Freshwater and Seawater Media , 2005 .

[170]  Yuan-Kun Lee,et al.  Effect of photobioreactor inclination on the biomass productivity of an outdoor algal culture , 1991, Biotechnology and bioengineering.

[171]  C. Lan,et al.  Closed photobioreactors for production of microalgal biomasses. , 2012, Biotechnology advances.

[172]  M. Huntley,et al.  CO2 Mitigation and Renewable Oil from Photosynthetic Microbes: A New Appraisal , 2007 .

[173]  J. Wood,et al.  Microbial resistance to heavy metals. , 1983, Environmental science & technology.

[174]  Aharon Gedanken,et al.  Bio-diesel production directly from the microalgae biomass of Nannochloropsis by microwave and ultrasound radiation. , 2011, Bioresource technology.

[175]  Johannes Tramper,et al.  Capturing sunlight into a photobioreactor: Ray tracing simulations of the propagation of light from capture to distribution into the reactor , 2008 .

[176]  Y. Chisti Biodiesel from microalgae. , 2007, Biotechnology advances.

[177]  F. G. Acién,et al.  Dynamic model of microalgal production in tubular photobioreactors. , 2012, Bioresource technology.

[178]  Kai Zhang,et al.  Optimized aeration by carbon dioxide gas for microalgal production and mass transfer characterization in a vertical flat-plate photobioreactor , 2002, Bioprocess and biosystems engineering.

[179]  T. Sharkey,et al.  Light-emitting diodes as a light source for photosynthesis research , 2004, Photosynthesis Research.

[180]  K. Williams,et al.  Microbiome analysis of a microalgal mass culture growing in municipal wastewater in a prototype OMEGA photobioreactor. , 2014 .

[181]  S. Miyachi,et al.  Outdoor culture of a cyanobacterium with a vertical flat-plate photobioreactor: effects on productivity of the reactor orientation, distance setting between the plates, and culture temperature , 1999, Applied Microbiology and Biotechnology.

[182]  John J. Milledge,et al.  Commercial application of microalgae other than as biofuels: a brief review , 2011 .

[183]  Yusuf Chisti,et al.  Shear stress tolerance and biochemical characterization of Phaeodactylum tricornutum in quasi steady-state continuous culture in outdoor photobioreactors. , 2003 .

[184]  C. Riquelme,et al.  Production of a diatom-bacteria biofilm in a photobioreactor for aquaculture applications , 2007 .

[185]  M. Tredici,et al.  Outdoor mass culture of Spirulina maxima in sea-water , 1986, Applied Microbiology and Biotechnology.

[186]  Michael W. Fowler,et al.  A flat-sided photobioreactor for culturing microalgae , 1993 .

[187]  H Jupsin,et al.  Dynamic mathematical model of high rate algal ponds (HRAP). , 2003, Water science and technology : a journal of the International Association on Water Pollution Research.

[188]  L. Rodolfi,et al.  Growth of the toxic dinoflagellate Alexandrium minutum (Dinophyceae) using high biomass culture systems , 2002, Journal of Applied Phycology.

[189]  René H. Wijffels,et al.  Design Process of an Area-Efficient Photobioreactor , 2008, Marine Biotechnology.

[190]  Yuan-Kun Lee,et al.  Design and performance of an α-type tubular photobioreactor for mass cultivation of microalgae , 1995, Journal of Applied Phycology.

[191]  J. Ogbonna,et al.  Improvement of mass transfer characteristics and productivities of inclined tubular photobioreactors by installation of internal static mixers , 2002, Applied Microbiology and Biotechnology.

[192]  Richard E. Moore,et al.  Structure determination, conformational analysis, chemical stability studies, and antitumor evaluation of the cryptophycins. Isolation of 18 new analogs from Nostoc sp. strain GSV 224 , 1995 .

[193]  Qiang Hu,et al.  Handbook of Microalgal Culture: Applied Phycology and Biotechnology , 2013 .

[194]  J. Doucha,et al.  Productivity, CO2/O2 exchange and hydraulics in outdoor open high density microalgal (Chlorella sp.) photobioreactors operated in a Middle and Southern European climate , 2006, Journal of Applied Phycology.

[195]  Graziella Chini Zittelli,et al.  Productivity and photosynthetic efficiency of outdoor cultures of Tetraselmis suecica in annular columns , 2006 .

[196]  J. Masojídek,et al.  A closed solar photobioreactor for cultivation of microalgae under supra-high irradiance: basic design and performance , 2003, Journal of Applied Phycology.

[197]  Manuel Berenguel,et al.  Model predictive control of pH in tubular photobioreactors , 2004 .