Production of transgenic maize plants and progeny by bombardment of hi-II immature embryos

SummaryProduction of transgenic maize (Zea mays L.) callus, plants, and progeny from microprojectile bombardment of 2–5-d cultured Hi-II immature embryos is described. Histological evidence indicates that these tissues are amenable to transformation due to surface layer cell division of the scutellum. Two out of every 100 bombarded embryos produced transgenic callus and R0 transgenic plants were both male and female fertile. Expected segregation of transgenes was observed in progeny. The primary advantage of bombarding these tissues is increased male and female fertility of transgenic plants compared with those produced using long-term callus or suspension cultures.

[1]  P. Christou,et al.  Molecular and genetic characterization of elite transgenic rice plants produced by electric-discharge particle acceleration , 2004, Theoretical and Applied Genetics.

[2]  R. Lundquist,et al.  Transformation and inheritance of a hygromycin phosphotransferase gene in maize plants , 2004, Plant Molecular Biology.

[3]  L. Crossland,et al.  Field Performance of Elite Transgenic Maize Plants Expressing an Insecticidal Protein Derived from Bacillus thuringiensis , 1993, Bio/Technology.

[4]  C. Somerville,et al.  Transformation with a mutant Arabidopsis acetolactate synthase gene renders tobacco resistant to sulfonylurea herbicides , 1988, Molecular and General Genetics MGG.

[5]  李宝健.  INTRODUCTION OF FOREIGN GENES INTO THE SEED EMBRYO CELLS OF RICE BY ELECTRO-INJECTION AND THE REGENERATION OF TRANSGENIC RICE PLANTS , 1991 .

[6]  P. Lemaux,et al.  Transformation of Maize Cells and Regeneration of Fertile Transgenic Plants. , 1990, The Plant cell.

[7]  D. Shah,et al.  The structure and expression of maize genes encoding the major heat shock protein, hsp70 , 1986, The EMBO journal.

[8]  V. Kauppinen,et al.  Fertile transgenic barley by particle bombardment of immature embryos , 2004, Plant Molecular Biology.

[9]  R. Phillips,et al.  Genetic Implications of Somaclonal Variation in Plants , 1992 .

[10]  M. Fromm,et al.  Factors Influencing Gene Delivery into Zea Mays Cells by High–Velocity Microprojectiles , 1988, Bio/Technology.

[11]  C. Fauquet,et al.  An improved rice transformation system using the biolistic method , 1993, Plant Cell Reports.

[12]  P. Lemaux,et al.  Generation of Large Numbers of Independently Transformed Fertile Barley Plants , 1994, Plant physiology.

[13]  M. Fromm,et al.  Transient expression of GUS and anthocyanin constructs in intact maize immature embryos following electroporation , 1993, Plant Cell, Tissue and Organ Culture.

[14]  O. Anderson,et al.  Rapid Production of Multiple Independent Lines of Fertile Transgenic Wheat (Triticum aestivum) , 1993, Plant physiology.

[15]  C. Armstrong,et al.  AgNO3 increases type II callus production from immature embryos of maize inbred B73 and its derivatives , 1991, Plant Cell Reports.

[16]  V. Walbot,et al.  Introns increase gene expression in cultured maize cells. , 1987, Genes & development.

[17]  P. Christou,et al.  Production of Transgenic Rice (Oryza Sativa L.) Plants from Agronomically Important Indica and Japonica Varieties via Electric Discharge Particle Acceleration of Exogenous DNA into Immature Zygotic Embryos , 1991, Bio/Technology.

[18]  M. Fromm,et al.  Inheritance and Expression of Chimeric Genes in the Progeny of Transgenic Maize Plants , 1990, Bio/Technology.

[19]  H. Machii,et al.  Production of fertile transgenic barley (Hordeum vulgare L.) plant using the hygromycin-resistance marker , 1995, Plant Cell Reports.

[20]  M. Bevan,et al.  GUS fusions: beta‐glucuronidase as a sensitive and versatile gene fusion marker in higher plants. , 1987, The EMBO journal.

[21]  C. Armstrong,et al.  Development and availability of germplasm with high Type II culture formation response , 1991 .

[22]  Kathleen Y. Lee,et al.  The molecular basis of sulfonylurea herbicide resistance in tobacco , 1988, The EMBO journal.

[23]  C. Chu,et al.  ESTABLISHMENT OF AN EFFICIENT MEDIUM FOR ANTHER CULTURE OF RICE THROUGH COMPARATIVE EXPERIMENTS ON THE NITROGEN SOURCES , 1975 .

[24]  C. L. Armstrong,et al.  Establishment and maintenance of friable, embryogenic maize callus and the involvement of L-proline , 1985, Planta.

[25]  P. Hasegawa,et al.  Transgenic sorghum plants via microprojectile bombardment. , 1993, Proceedings of the National Academy of Sciences of the United States of America.

[26]  G. Galili,et al.  Improvement of plant regeneration and GUS expression in scutellar wheat calli by optimization of culture conditions and DNA-microprojectile delivery procedures , 1992, Molecular and General Genetics MGG.

[27]  J. Leemans,et al.  Transgenic maize plants by tissue electroporation. , 1992, The Plant cell.