Improved Cellulase Production by Trichoderma reesei RUT C30 under SSF Through Process Optimization

The major constraint in the enzymatic saccharification of biomass for ethanol production is the cost of cellulase enzymes. Production cost of cellulases may be brought down by multifaceted approaches which includes the use of cheap lignocellulosic substrates for fermentation production of the enzyme, and the use of cost efficient fermentation strategies like solid state fermentation (SSF). The current study investigated the production of cellulase by Trichoderma reesei RUT C30 on wheat bran under SSF. Process parameters important in cellulase production were identified by a Plackett and Burman design and the parameters with significant effects on enzyme production were optimized for maximal yield using a central composite rotary design (CCD). Higher initial moisture content of the medium had a negative effect on production whereas incubation temperature influenced cellulase production positively in the tested range. Optimization of the levels of incubation temperature and initial moisture content of the medium resulted in a 6.2 fold increase in production from 0.605 to 3.8 U/gds of cellulase. The optimal combination of moisture and temperature was found to be 37.56% and 30 °C, respectively, for maximal cellulase production by the fungus on wheat bran.

[1]  W. Verstraete,et al.  Anaerobic solid state fermentation of cellulosic substrates with possible application to cellulase production , 1987, Applied Microbiology and Biotechnology.

[2]  P. Chahal,et al.  Cellulase production profile of Trichoderma reesei on different cellulosic substrates at various pH levels , 1992 .

[3]  D. Gokhale,et al.  Polysaccharides from bagasse: applications in cellulase and xylanase production , 2004 .

[4]  D. Gregg,et al.  Techno-economic evaluations of a generic wood-to-ethanol process: effect of increased cellulose yields and enzyme recycle , 1998 .

[5]  Michael Wang,et al.  Effects of Fuel Ethanol Use on Fuel-Cycle Energy and Greenhouse Gas Emissions , 1999 .

[6]  Wei Liao,et al.  Production of cellulase by Trichoderma reesei from dairy manure. , 2005, Bioresource technology.

[7]  M. Penttilä,et al.  Regulation of cellulase gene expression in the filamentous fungus Trichoderma reesei , 1997, Applied and environmental microbiology.

[8]  M. Raimbault,et al.  General and microbiological aspects of solid substrate fermentation , 1998 .

[9]  D. S. Chahal.  Solid-State Fermentation with Trichoderma reesei for Cellulase Production , 1985, Applied and environmental microbiology.

[10]  Poonam Singh Nee Nigam,et al.  Processing of agricultural wastes in solid state fermentation for cellulolytic enzymes production , 1996 .

[11]  E. Galindo,et al.  Production of 6-pentyl-α-pyrone by Trichoderma harzianum cultured in unbaffled and baffled shake flasks , 2004 .

[12]  W. D. Murray,et al.  Bioconversion of forest products industry waste cellulosics to fuel ethanol: a review. , 1996 .

[13]  M. Penttilä,et al.  Production of Trichoderma reesei cellulases on glucose-containing media. , 1995, Applied and environmental microbiology.

[14]  R. M. Lafferty,et al.  The use of lignocellulosic wastes for production of cellulase by Trichoderma reesei , 1987, Applied Microbiology and Biotechnology.

[15]  C. Soccol,et al.  New developments in solid state fermentation: I-bioprocesses and products. , 2000 .

[16]  R. Plackett,et al.  THE DESIGN OF OPTIMUM MULTIFACTORIAL EXPERIMENTS , 1946 .

[17]  Michael Knauf,et al.  Lignocellulosic biomass processing: a perspective. , 2004 .

[18]  M. Gutarra,et al.  Lipase production by solid-state fermentation , 2005, Applied biochemistry and biotechnology.

[19]  R. Tengerdy.  CELLULASE PRODUCTION BY SOLID SUBSTRATE FERMENTATION , 1996 .

[20]  M. Raimbault,et al.  Water and water activity in the solid state fermentation of cassava starch by Aspergillus niger , 1988, Applied Microbiology and Biotechnology.

[21]  G. Box,et al.  On the Experimental Attainment of Optimum Conditions , 1951 .

[22]  R. Tengerdy,et al.  Mixed culture solid substrate fermentation of Trichoderma reesei with Aspergillus niger on sugar cane bagasse , 1999 .

[23]  G. Zacchi,et al.  Cellulase production by T. reesei , 1996 .