In vitro reconstruction of the Aspergillus (= Emericella) nidulans genome.

A physical map of the 31-megabase Aspergillus nidulans genome is reported, in which 94% of 5,134 cosmids are assigned to 49 contiguous segments. The physical map is the result of a two-way ordering process, in which clones and probes were ordered simultaneously on a binary DNA/DNA hybridization matrix. Compression by elimination of redundant clones resulted in a minimal map, which is a chromosome walk. Repetitive DNA is nonrandomly dispersed in the A. nidulans genome, reminiscent of heterochromatic banding patterns of higher eukaryotes. We hypothesize gene clusters may arise by horizontal transfer and spread by transposition to explain the nonrandom pattern of repeats along chromosomes.

[1]  T. Marr,et al.  A 13 kb resolution cosmid map of the 14 Mb fission yeast genome by nonrandom sequence-tagged site mapping , 1993, Cell.

[2]  A. Cuticchia,et al.  The use of simulated annealing in chromosome reconstruction experiments based on binary scoring. , 1992, Genetics.

[3]  E. Lander,et al.  Genomic mapping by anchoring random clones: a mathematical analysis. , 1991, Genomics.

[4]  W. Timberlake,et al.  Sexual origins of British Aspergillus nidulans isolates. , 1994, Proceedings of the National Academy of Sciences of the United States of America.

[5]  J. Shea,et al.  Simultaneous identification of bacterial virulence genes by negative selection. , 1995, Science.

[6]  W. Timberlake,et al.  The Aspergillus nidulans yA gene is regulated by abaA. , 1993, The EMBO journal.

[7]  W. Timberlake,et al.  Clustering of spore-specific genes in Aspergillus nidulans. , 1982, Proceedings of the National Academy of Sciences of the United States of America.

[8]  D. Hibbett,et al.  Phylogenetic evidence for horizontal transmission of group I introns in the nuclear ribosomal DNA of mushroom-forming fungi. , 1996, Molecular biology and evolution.

[9]  W. Timberlake,et al.  A large cluster of highly expressed genes is dispensable for growth and development in Aspergillus nidulans. , 1989, Genetics.

[10]  T G Marr,et al.  Genome mapping by nonrandom anchoring: a discrete theoretical analysis. , 1993, Proceedings of the National Academy of Sciences of the United States of America.

[11]  D. Hartl,et al.  Optimized strategies for sequence-tagged-site selection in genome mapping. , 1991, Proceedings of the National Academy of Sciences of the United States of America.

[12]  W. Timberlake,et al.  Genetic engineering of filamentous fungi. , 1989, Science.

[13]  W. Timberlake,et al.  The Aspergillus nidulans brlA regulatory locus consists of overlapping transcription units that are individually required for conidiophore development. , 1993, The EMBO journal.

[14]  J. Manners,et al.  Evidence for chromosome transfer between biotypes of Colletotrichum gloeosporioides , 1994 .

[15]  J. Arnold.  Cytonuclear Disequilibria in Hybrid Zones , 1993 .

[16]  B. Rutledge,et al.  DNA sequence, organization and regulation of the qa gene cluster of Neurospora crassa. , 1989, Journal of molecular biology.

[17]  P. Renault,et al.  Mosaic structure of large regions of the Lactococcus lactis subsp. cremoris chromosome. , 1994, Microbiology.

[18]  N. Morris.  Nucleosome structure in Aspergillus nidulans , 1976, Cell.

[19]  J. Griffith,et al.  On the consistency of a physical mapping method to reconstruct a chromosome in vitro. , 1996, Genetics.

[20]  R. Dean,et al.  Production of cell wall-degrading enzymes by Aspergillus nidulans: a model system for fungal pathogenesis of plants. , 1989, The Plant cell.

[21]  J R Roth,et al.  Selfish operons: horizontal transfer may drive the evolution of gene clusters. , 1996, Genetics.

[22]  J. Davies,et al.  Construction and pathogenicity of Aspergillus fumigatus mutants that do not produce the ribotoxin restrictocin , 1993, Molecular microbiology.

[23]  L Kruglyak,et al.  An STS-Based Map of the Human Genome , 1995, Science.

[24]  W. Timberlake.  Temporal and spatial controls of Aspergillus development. , 1991, Current opinion in genetics & development.

[25]  W. Timberlake,et al.  Structure and regulated expression of the SpoC1 gene cluster from Aspergillus nidulans. , 1984, Journal of molecular biology.

[26]  A. Cuticchia,et al.  Chromosome-specific recombinant DNA libraries from the fungus Aspergillus nidulans. , 1991, Nucleic acids research.

[27]  T. C. Nesbitt,et al.  Twenty-five coregulated transcripts define a sterigmatocystin gene cluster in Aspergillus nidulans. , 1996, Proceedings of the National Academy of Sciences of the United States of America.

[28]  A. Clutterbuck.  A mutational analysis of conidial development in Aspergillus nidulans. , 1969, Genetics.