Sero-prevalence of Johne's disease in bovine breeding bulls using indigenous ELISA kit.

Indigenous ELISA kit developed using protoplasmic antigen from native Mycobacterium avium subsp. paratuberculosis ‘Bison Type’ strain of goat origin was adapted to screen bovine bulls. Serum samples were collected from 49 breeding bulls from dairy farms in Ludhiana (Punjab). The study showed moderate rate of sero-prevalence of Johne’s disease in dairy farm bulls (14.2%). Using S/P ratio, bulls were grouped as negative, suspected, low positive, moderately positive and strong positive for Johne’s disease in 12.2, 6.1, 8.1, 59.1 and 14.2 samples, respectively. The prevalence rate was high (73.4%) in Type II category of sero-reactors. Using ELISA kit, Type 1 sero-rectors had better correlation with fecal culture as compared to Type II sero-reactors, therefore, recommended for estimating sero-prevalence. As first step to control Johne’s disease, removal of strong positives sero-reactors may help to reduce shedder bulls from herds. Bulls in suspected, low positive and moderately positive categories may be vaccinated against Johne’s disease as second step of control measures.

[1]  P. Singh,et al.  Pathogenic 'Bison-type' Mycobacterium avium subspecies paratuberculosis genotype characterized from riverine buffalo (Bubalus bubalis) in North India. , 2008, Comparative immunology, microbiology and infectious diseases.

[2]  V. Gupta,et al.  Juvenile Capri-Paratuberculosis (JCP) in India: Incidence and characterization by six diagnostic tests , 2007 .

[3]  N. Singh.,et al.  Evaluation of an indigenous ELISA for diagnosis of Johne’s disease and its comparison with commercial kits , 2007, Indian Journal of Microbiology.

[4]  V. Gupta,et al.  Sero-prevalence of Johne's disease in prospective young bulls of Hariana breed by indigenous ELISA kit, using protoplasmic antigen from 'Bison type' genotype of Mycobacterium avium subspecies paratuberculosis of goat origin , 2007 .

[5]  Sachin Kumar,et al.  Sero-prevalence of paratuberculosis in young kids using ‘Bison type’, Mycobacterium avium subsp. paratuberculosis antigen in plate ELISA , 2007 .

[6]  B. N. Tripathi,et al.  Detection of Mycobacterium avium subsp. paratuberculosis in intestinal and lymph node tissues of water buffaloes (Bubalus bubalis) by PCR and bacterial culture. , 2005, Veterinary microbiology.

[7]  M. Salgado,et al.  Performance of a Johne's Disease Enzyme-Linked Immunosorbent Assay Adapted for Milk Samples from Goats , 2005, Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc.

[8]  C. Buergelt,et al.  Identification of Mycobacterium avium subspecies paratuberculosis by Polymerase Chain Reaction in Blood and Semen of a Bull with Clinical Paratuberculosis , 2004 .

[9]  M. Collins.  Interpretation of a Commercial Bovine Paratuberculosis Enzyme-Linked Immunosorbent Assay by Using Likelihood Ratios , 2002, Clinical and Vaccine Immunology.

[10]  P. Ahrens,et al.  Detection of Mycobacterium avium subsp. paratuberculosis in milk from clinically affected cows by PCR and culture. , 2000, Veterinary microbiology.

[11]  L. Alban,et al.  A cross-sectional study of paratuberculosis in 1155 Danish dairy cows. , 2000, Preventive veterinary medicine.

[12]  J. Sheridan,et al.  Causation of Crohn's disease by Mycobacterium avium subspecies paratuberculosis. , 2000, Canadian journal of gastroenterology = Journal canadien de gastroenterologie.

[13]  J. Stabel,et al.  Johne's disease: a hidden threat. , 1998, Journal of dairy science.

[14]  R. Streeter,et al.  Isolation of Mycobacterium paratuberculosis from colostrum and milk of subclinically infected cows. , 1995, American journal of veterinary research.

[15]  R. Sweeney,et al.  Diagnosis of paratuberculosis in dairy cattle, using enzyme-linked immunosorbent assay for detection of antibodies against Mycobacterium paratuberculosis in milk. , 1994, American journal of veterinary research.

[16]  W J Goodger,et al.  Herd prevalence and geographic distribution of, and risk factors for, bovine paratuberculosis in Wisconsin. , 1994, Journal of the American Veterinary Medical Association.

[17]  C. Wilks,et al.  Isolation of Mycobacterium paratuberculosis from the milk of a cow with Johne's disease , 1981, Veterinary Record.

[18]  R. C. Kulshreshtha,et al.  A study on the prevalence of tuberculosis and Johne's disease in cattle and buffaloes in Haryana State. , 1980 .