Alterations of cellular characteristics of a human ovarian teratocarcinoma cell line after in vitro treatment with retinoids.

Differentiation of the human teratocarcinoma derived cell line. PA-1, with retinoids was examined at concentrations (10(-6)-10(-8) M) that did not exhibit an antiproliferative effect during log-phase growth. Treatment with naturally occurring retinoic acid or certain synthetic retinoids (13-cis retinoic acid, Ro10-9359, and Ro13-7410), while not significantly altering the log-phase growth rate, decreased the saturation cell density and mitotic indices after confluence. Retinoid treatment also induced changes in cell morphology, which appear to be related to reorganization of microtubules and microfilaments. Following retinoid treatment, the expression of cell glycoproteins (of 162 kDa, 152 kDa, 143 kDa. and 51 kDa) was altered. Treated cells also exhibited decreased expression of alkaline phosphatase, as well as an increased capacity for intercellular communication as evidenced by gap-junctional transfer of the phosphorylated toxic intermediate of 6-thioguanine to HPRT- cells. Treatment with retinoic acid dramatically reduced the quantity of shed plasma membrane material and altered its composition.

[1]  Douglas D. Taylor,et al.  Characterization of plasma membrane shedding from murine melanoma cells , 1988, International journal of cancer.

[2]  J. Kalin,et al.  In vitro effects of retinoids on murine thymus-dependent and thymus-independent mitogenesis. , 1987, Cellular immunology.

[3]  S. Lippman,et al.  Retinoids as preventive and therapeutic anticancer agents (Part I). , 1987, Cancer treatment reports.

[4]  C. C. Marvel,et al.  Effect of retinoic acid and phorbol-12-myristate-13-acetate on glycosyltransferase activities in normal and transformed cells. , 1987, Cancer research.

[5]  J. Barrett,et al.  Differential response to retinoic acid of Syrian hamster embryo fibroblasts expressing v-src or v-Ha-ras oncogenes , 1986, Molecular and cellular biology.

[6]  M. Reiss,et al.  Modulation of cell shedding and glycosaminoglycan synthesis of human malignant keratinocytes by all-trans-retinoic acid and hydrocortisone in vitro. , 1986, The Journal of investigative dermatology.

[7]  S. Eccles.  Effects of retinoids on growth and dissemination of malignant tumours: immunological considerations. , 1985, Biochemical pharmacology.

[8]  T. J. Lindell,et al.  Inhibition by retinoic acid of murine retrovirus-induced cellular transformation and tumor formation. , 1985, Journal of the National Cancer Institute.

[9]  C. Cooper,et al.  An activated rasN gene: detected in late but not early passage human PA1 teratocarcinoma cells. , 1984, Science.

[10]  E. Rapaport,et al.  Retinoic acid alters subcellular compartmentalization of ATP pools in 3T3 cells but not in HeLa cells , 1984, Journal of cellular physiology.

[11]  J. Shaw,et al.  Microtubule disassembly and morphologic alterations induced by 1-chloro-2,4-dinitrobenzene, a substrate for glutathione S-transferase. , 1984, Cell biology international reports.

[12]  D. M. Darby,et al.  Cell surface characteristics of an allotransplantable TA3 ascites subline resulting from a process of immunoselection. , 1983, Cancer research.

[13]  D. Taylor,et al.  Isolation of plasma membrane fragments from cultured murine melanoma cells. , 1983, Biochemical and biophysical research communications.

[14]  E. Adamson,et al.  Epithelial layer formation in differentiating aggregates of F9 embryonal carcinoma cells , 1983, The Journal of cell biology.

[15]  P. Medawar,et al.  Anti-cancer action of retinoids. , 1981, Immunology.

[16]  P. Avner,et al.  Characterization of a human ovarian teratocarcinoma‐derived cell line , 1980, International journal of cancer.

[17]  J. Collins,et al.  Anti‐tumor potential of retinoic acid: Stimulation of immune mediated effectors , 1979, International journal of cancer.

[18]  S. Strickland,et al.  The induction of differentiation in teratocarcinoma stem cells by retinoic acid , 1978, Cell.

[19]  P. Avner,et al.  Isolation of a human teratoma cell line which expresses F9 antigen , 1977, Nature.

[20]  R. Lotan,et al.  Inhibitory effects of retinoic acid or retinyl acetate on the growth of untransformed, transformed, and tumor cells in vitro. , 1977, Journal of the National Cancer Institute.

[21]  Franclois Jacob.  Mouse Teratocarcinoma and Embryonic Antigens , 1977, Immunological reviews.

[22]  B. Giovanella,et al.  Heterotransplantation of human malignant tumors in "nude" thymusless mice. II. Malignant tumors induced by injection of cell cultures derived from human solid tumors. , 1974, Journal of the National Cancer Institute.

[23]  U. K. Laemmli,et al.  Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 , 1970, Nature.

[24]  Oliver H. Lowry,et al.  Protein measurement with the Folin phenol reagent. , 1951, The Journal of biological chemistry.

[25]  D. Taylor,et al.  Shedding of plasma membrane fragments. Neoplastic and developmental importance. , 1986, Developmental biology.

[26]  A. Jetten.  Modulation of cell growth by retinoids and their possible mechanisms of action. , 1984, Federation proceedings.

[27]  A. Bloch.  Induced cell differentiation in cancer therapy. , 1984, Cancer treatment reports.

[28]  W. Fishman,et al.  The placental isoenzymes of alkaline phosphatase in sera of normal pregnancy. , 1972, American journal of clinical pathology.