A rapid microneutralization assay for the measurement of neutralizing antibody reactive with human cytomegalovirus.

We have developed a murine monoclonal antibody reactive with a major immediate early 72,000 dalton protein of human cytomegalovirus and utilized this reagent in a rapid virus titration and microneutralization assay. Because of the early expression of this virus encoded protein, both assays could be accomplished within 16 h following virus inoculation. In addition, both assays resulted in considerable savings of reagents because the assays were carried out in 96-well microtiter plates. These assays should prove useful in the preparation and study of neutralizing antibodies directed against human cytomegalovirus.

[1]  K. Scott,et al.  Rapid quantitation of cytomegalovirus and assay of neutralizing antibody by using monoclonal antibody to the major immediate-early viral protein , 1988, Journal of clinical microbiology.

[2]  W. Britt,et al.  Structural and immunological characterization of the intracellular forms of an abundant 68,000 Mr human cytomegalovirus protein. , 1987, The Journal of general virology.

[3]  G. Alpert,et al.  Rapid detection of human cytomegalovirus in the urine of humans. , 1985, The Journal of infectious diseases.

[4]  S. Plotkin,et al.  A rapid microneutralization assay for cytomegalovirus. , 1986, Journal of virological methods.

[5]  D. R. Thomsen,et al.  Organization and expression of the immediate early genes of human cytomegalovirus , 1983, Journal of virology.

[6]  J. Meyers,et al.  Evaluation of a direct fluorescein-conjugated monoclonal antibody for detection of cytomegalovirus in centrifugation culture , 1987, Journal of clinical microbiology.

[7]  W. Britt.  Neutralizing antibodies detect a disulfide-linked glycoprotein complex within the envelope of human cytomegalovirus. , 1984, Virology.

[8]  W. Britt,et al.  Synthesis and processing of the envelope gp55-116 complex of human cytomegalovirus , 1986, Journal of virology.

[9]  W. Britt,et al.  Identification of a 65 000 dalton virion envelope protein of human cytomegalovirus. , 1985, Virus research.

[10]  J. Waner,et al.  Analysis of antigenic diversity among human cytomegaloviruses by kinetic neutralization tests with high-titered rabbit antisera , 1978, Infection and immunity.