Butanol Production from Lignocellulosic-based Hexoses and Pentoses by Fermentation of Clostridium Acetobutylicum

The Acetone-Butanol-Ethanol (ABE) fermentation is receiving renewed interest as a way to upgrade renewable resources into valuable base chemicals and liquid fuels. Abundance and un-competitiveness with food sources are desired features of a potential substrate, and they are met by lignocellulosic biomass.This contribution is about the characterization of the ABE fermentation by C. acetobutylicum DSM 792 using sugars representative for hydrolysis products of lignocellulosic biomass: hexoses (glucose and mannose) and pentoses (arabinose and xylose).Batch fermentations of single sugars were carried out. The conversion process was characterized as a function of time in terms of biomass, acids and solvents concentrations as well as pH and total organic compounds. C. acetobutylicum was able to convert all investigated sugars. The conversion into solvents was strongly enhanced by the presence of CaCO3 in the fermentation medium.

[1]  Michael C. Flickinger,et al.  Encyclopedia of bioprocess technology : fermentation, biocatalysis, and bioseparation , 1999 .

[2]  Stefan Junne.  Stimulus response experiments for modelling product formation in Clostridium acetobutylicum fermentations , 2010 .

[3]  H. Blaschek,et al.  Effect of Acetate on Molecular and Physiological Aspects of Clostridium beijerinckii NCIMB 8052 Solvent Production and Strain Degeneration , 1999, Applied and Environmental Microbiology.

[4]  Thaddeus Chukwuemeka Ezeji,et al.  Production of acetone, butanol and ethanol by Clostridium beijerinckii BA101 and in situ recovery by gas stripping , 2003 .

[5]  L. Nielsen,et al.  Fermentative butanol production by clostridia , 2008, Biotechnology and bioengineering.

[6]  T. Ezeji,et al.  Butanol Production from Lignocellulosic Biomass , 2010 .

[7]  Kalyan Gayen,et al.  Developments in biobutanol production: New insights , 2011 .

[8]  T. Ezeji,et al.  Fermentation of dried distillers' grains and solubles (DDGS) hydrolysates to solvents and value-added products by solventogenic clostridia. , 2008, Bioresource technology.

[9]  Bioenergy II: An Assessment of the Kinetics of Butanol Production by Clostridium acetobutylicum , 2009 .

[10]  T. Ezeji,et al.  Stimulatory effects of calcium carbonate on butanol production by solventogenic Clostridium species. , 2011 .

[11]  D. Ross.  The acetone-butanol fermentation. , 1961, Progress in industrial microbiology.

[12]  D. T. Jones,et al.  Acetone-butanol fermentation revisited. , 1986, Microbiological reviews.

[13]  P. Dürre.  Biobutanol: An attractive biofuel , 2007, Biotechnology journal.

[14]  N. Qureshi,et al.  Economics of Butanol Fermentation using Hyper-Butanol Producing Clostridium Beijerinckii BA101 , 2000 .

[15]  Nasib Qureshi,et al.  Butanol production from wheat straw hydrolysate using Clostridium beijerinckii , 2007, Bioprocess and biosystems engineering.

[16]  Nathan D. Price,et al.  Achievements and perspectives to overcome the poor solvent resistance in acetone and butanol-producing microorganisms , 2010, Applied Microbiology and Biotechnology.

[17]  G. Gottschalk,et al.  Physiological Events in Clostridium acetobutylicum during the Shift from Acidogenesis to Solventogenesis in Continuous Culture and Presentation of a Model for Shift Induction , 1992, Applied and environmental microbiology.

[18]  Ron Cascone,et al.  Biobutanol : A Replacement for Bioethanol? , 2008 .

[19]  G. Raval,et al.  Regulation and butanol inhibition of D-xylose and D-glucose uptake in Clostridium acetobutylicum , 1985, Applied and environmental microbiology.