Experimental colonic inflammation and ulceration. Permeation of a water-soluble contrast medium as a measure of 'disease' activity.

The aim of this study was to investigate the permeation of an isosmolar water-soluble X-ray contrast medium (CM) from the unperforated colon after experimental induction of inflammation and ulceration. One hundred and sixty-five male rats were included. In 110 rats 0.25 ml of 40% ethanol + 15 mg or 30 mg trinitrobenzene (TNB) was instilled into the colon, 7-9 cm proximal to the anus, inducing different degrees of inflammation. Fifty-five rats served as controls and had 0.25 ml saline instilled with the same procedure. At 7, 14, 21, and 28 days 3 ml of the non-ionic CM iodixanol was applied as an enema, and subsequently all urine was collected for the next 4 h. High-performance liquid chromatography analyses of the urine showed a positive relation between the severity of inflammation/ulceration in the colonic wall as assessed by a macroscopic damage score and the amount of CM excreted in the urine. Water-soluble contrast media may have prospects of combining functional tests of intestinal membrane dysfunction with segmental exposure control.

[1]  F. Laerum,et al.  Intestinal follow-through examinations with iohexol and iopentol. Permeability alterations and efficacy in patients with small bowel obstruction. , 1991, Investigative radiology.

[2]  F. Laerum,et al.  Urinary excretion of iohexol after enteral administration in rats with radiation injury of the small intestine. , 1991, Scandinavian journal of gastroenterology.

[3]  G. Olaison,et al.  Abnormal intestinal permeability in Crohn's disease. A possible pathogenic factor. , 1990, Scandinavian journal of gastroenterology.

[4]  P. Gaulard,et al.  MONOCLONAL ANTIBODY (HML-1) REACTIVITY OF T-CELL LYMPHOMAS , 1989, The Lancet.

[5]  T. Peters,et al.  Intestinal permeability, non-steroidal anti-inflammatory drug enteropathy and inflammatory bowel disease: an overview. , 1989, Gut.

[6]  O. B. Schaffalitzky de Muckadell,et al.  Intestinal permeability of 51Cr-labelled ethylenediaminetetraacetic acid in patients with Crohn's disease and their healthy relatives. , 1989, Scandinavian journal of gastroenterology.

[7]  A. Axon,et al.  Cellobiose/mannitol sugar test--a sensitive tubeless test for coeliac disease: results on 1010 unselected patients. , 1989, Gut.

[8]  L. Aabakken.  51Cr-Ethylenediaminetetraacetic Acid Absorption Test: Methodologic Aspects , 1989 .

[9]  J. Wallace,et al.  Hapten-induced model of chronic inflammation and ulceration in the rat colon. , 1989, Gastroenterology.

[10]  D. Hollander,et al.  Crohn's disease--a permeability disorder of the tight junction? , 1988, Gut.

[11]  A. Stordahl.  Urinary excretion of enteral iohexol in rats with intestinal ischaemia. The influence of size of ischaemic area and duration of exposure to contrast medium. , 1988, Scandinavian journal of gastroenterology.

[12]  A. Stordahl.  Urinary excretion of iohexol administered enterally in rats with intestinal ischaemia. A transmural and transperitoneal route of transport. , 1988, Scandinavian journal of gastroenterology.

[13]  F. Laerum,et al.  Water-soluble contrast media compared with barium in enteric follow-through. Urinary excretion and radiographic efficacy in rats with intestinal ischemia. , 1988, Investigative radiology.

[14]  Y. Minaire,et al.  Assessment of the lactulose-mannitol test in Crohn's disease. , 1988, Gut.

[15]  R. Hunt,et al.  Changes in intestinal permeability and epithelial differentiation during inflammation in the rat. , 1988, Gut.

[16]  D. Jones,et al.  Increased intestinal permeability in patients with rheumatoid arthritis: a side-effect of oral nonsteroidal anti-inflammatory drug therapy? , 1987, British journal of rheumatology.

[17]  J. Rotter,et al.  Increased intestinal permeability in patients with Crohn's disease and their relatives. A possible etiologic factor. , 1986, Annals of internal medicine.

[18]  A. Levi,et al.  Effect of non-steroidal anti-inflammatory drugs and prostaglandins on the permeability of the human small intestine. , 1986, Gut.

[19]  C. O'Morain,et al.  Chromium 51-ethylenediaminetetraacetate test: a useful test in the assessment of inflammatory bowel disease. , 1986, The Journal of laboratory and clinical medicine.

[20]  T. Peters,et al.  A PERSISTENT DEFECT IN INTESTINAL PERMEABILITY IN COELIAC DISEASE DEMONSTRATED BY A 51Cr-LABELLED EDTA ABSORPTION TEST , 1983, The Lancet.

[21]  D. Macdonald,et al.  INTESTINAL PERMEABILITY IN PATIENTS WITH ECZEMA AND FOOD ALLERGY , 1981, The Lancet.

[22]  R. Pounder,et al.  ABNORMAL INTESTINAL PERMEABILITY TO SUGARS IN VILLOUS ATROPHY , 1979, The Lancet.

[23]  A. Hofmann,et al.  Measurements of intestinal permeability using low molecular weight polyethylene glycols (PEG 400). I. Chemical analysis and biological properties of PEG 400. , 1977, Gastroenterology.

[24]  A. Hofmann,et al.  Measurements of intestinal permeability using low molecular weight polyethylene glycols (PEG 400). II. Application to normal and abnormal permeability states in man and animals. , 1977, Gastroenterology.

[25]  T. Ma,et al.  PEG 400, a hydrophilic molecular probe for measuring intestinal permeability. , 1990, Gastroenterology.

[26]  K. Fälth‐magnusson,et al.  Intestinal permeability assessed with different-sized polyethylene glycols in children undergoing small-intestinal biopsy for suspected celiac disease. , 1989, Scandinavian journal of gastroenterology.

[27]  T. Jacobsen,et al.  Determination of iopentol and other non-ionic contrast agents in biologic fluids by high-performance liquid chromatography. , 1987, Acta radiologica. Supplementum.

[28]  G. Palace,et al.  High-performance liquid chromatographic determination of iohexol in plasma, urine and feces. , 1983, Journal of chromatography.

[29]  S. Ukabam,et al.  Abnormal small intestinal permeability to sugars in patients with Crohn's disease of the terminal ileum and colon. , 1983, Digestion.