Qualitative, quantitative and pharmacokinetic study on the absorbed components of Ardisia japonica (Thunb.) Blume in rat plasma based on Molecular Networking combined with Quadrupole Time of Flight LC/MS and Triple Quadrupole LC/MS.

Isolation and screening of different compounds from extracts always the key of natural drug research and the absorbed prototype components has been considered as potential active ingredients. Ultra-high-performance liquid chromatography combined with quadrupole time-of-flight mass spectrometry (Q-TOF-LC/MS) has been widely used in research of natural drug but we still need a more effective tool to compare and treat from a raw data. In this study, we offered a fast analytic plan basing on Molecular networking (MN) for investigators to qualitative, quantitative the absorbed prototype components and their metabolites. Take Ardisia japonica (Thunb.) Blume as an example. As a result, total of 8 absorbed prototype components in rat plasma are identified. Futhermore, pharmacokinetic study also successfully carried out on the eight absorbed prototype components in rat plasma. Our findings have provided important information on the investigation of A. japonica in vivo. More importantly, the MS network analysis pattern serves as an integral solution for qualitative and quantitative phytochemical compounds of natural drugs.